Expansion of CD3+CD56+ lymphocytes correlates with induction of cytotoxicity by interleukin-2 gene transfer in human breast tumor cultures

Euhus, David M.; Kimura, Lucille H.; Arnold, Bruce

doi:10.1007/bf02305558

Cited by 8 publications

(4 citation statements)

References 20 publications

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“…Also, persons with a lower CD4/CD8 ratio were found to have higher numbers of these CD3+CD56+lymphocytes in their blood. These natural killer-like T-lymphocytes play an important role in the immunologic reaction against tumours (Lee et al 1996, Schmidt-Wolf et al 1997, Euhus et al 1997). CD3+CD56+lymphocytes were found in elevated numbers in the peripheral blood of patients suffering from ovarian cancer (Schroder et al 1997) or from colorectal cancer (Takii et al 1994) and in at least a subset of patients with monoclonal gammopathies (Famularo et al 1992).…”

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confidence: 99%

Immunologic and other biological parameters as a function of smoking status and of residence in areas differing in terms of air pollution

Larebeke

Husson

Coen

et al. 2003

International Journal of Environmental Health Research

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In the industrial region of La Louvière (Belgium), healthy persons, presenting at a consultation of preventive medicine, showed differences as a function of smoking and of residence in one of four areas differing in sources of emissions, population density and green zones. Smokers had significantly (P/=2.25, significantly so for the Power Station-Landfill area. A non-significant but consistent trend towards higher lymphocyte and CD8 lys counts and higher complement C3c serum levels further suggested an association between residence in a more polluted area and immunologic features. The number of CD3+CD56+ lys microl(-1), reported to be increased in cancer patients, showed a negative correlation with the CD4/CD8 ratio (r(2)=0.132, P<0.0001).

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mentioning

confidence: 99%

Immunologic and other biological parameters as a function of smoking status and of residence in areas differing in terms of air pollution

Larebeke

Husson

Coen

et al. 2003

International Journal of Environmental Health Research

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“…Other studies have also reported that NKT-cell proliferation is IL2-dependent [ 108 , 109 ]. For example, breast cancer cells expressing IL2 were shown to cause the expansion of NKT-cells in vitro [ 108 ]. Furthermore, IL2-secreting fibroblasts were shown to increase the NKT-cell counts in patients with peripheral neuroectodermal tumor and, subsequently tumor cell death [ 109 ].…”

Section: Discussionmentioning

confidence: 98%

“…NKT-cells are cytotoxic against tumors, where killing capacity is independent of the T-cell receptor activation [47]. Other studies have also reported that NKT-cell proliferation is IL2-dependent [108,109]. For example, breast cancer cells expressing IL2 were shown to cause the expansion of NKT-cells in vitro [108].…”

Section: Discussionmentioning

confidence: 99%

Human Mesenchymal Stem Cells Overexpressing Interleukin 2 Can Suppress Proliferation of Neuroblastoma Cells in Co-Culture and Activate Mononuclear Cells In Vitro

et al. 2020

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High-dose recombinant interleukin 2 (IL2) therapy has been shown to be successful in renal cell carcinoma and metastatic melanoma. However, systemic administration of high doses of IL2 can be toxic, causing capillary leakage syndrome and stimulating pro-tumor immune response. One of the strategies to reduce the systemic toxicity of IL2 is the use of mesenchymal stem cells (MSCs) as a vehicle for the targeted delivery of IL2. Human adipose tissue-derived MSCs were transduced with lentivirus encoding IL2 (hADSCs-IL2) or blue fluorescent protein (BFP) (hADSCs-BFP). The proliferation, immunophenotype, cytokine profile and ultrastructure of hADSCs-IL2 and hADSCs-BFP were determined. The effect of hADSCs on activation of peripheral blood mononuclear cells (PBMCs) and proliferation and viability of SH-SY5Y neuroblastoma cells after co-culture with native hADSCs, hADSCs-BFP or hADSCs-IL2 on plastic and Matrigel was evaluated. Ultrastructure and cytokine production by hADSCs-IL2 showed modest changes in comparison with hADSCs and hADSCs-BFP. Conditioned medium from hADSC-IL2 affected tumor cell proliferation, increasing the proliferation of SH-SY5Y cells and also increasing the number of late-activated T-cells, natural killer (NK) cells, NKT-cells and activated T-killers. Conversely, hADSC-IL2 co-culture led to a decrease in SH-SY5Y proliferation on plastic and Matrigel. These data show that hADSCs-IL2 can reduce SH-SY5Y proliferation and activate PBMCs in vitro. However, IL2-mediated therapeutic effects of hADSCs could be offset by the increased expression of pro-oncogenes, as well as the natural ability of hADSCs to promote the progression of some tumors.

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“…This cytokine also enhances the cytotoxic effect of γδ T-cells, increasing CD69 and degranulation marker CD107a expression and IFNγ secretion (Ribot et al, 2014). Furthermore, IL2 can also promote the expansion of NKT-cells in cancer patients (Euhus et al, 1997;Engel et al, 1998). IL2-based therapy has several significant drawbacks, particularly the stimulation of Tregs, which are associated with the suppression of the antitumor immune response (Boyman et al, 2006).…”

Section: Interleukinmentioning

confidence: 99%

Molecular Aspects and Future Perspectives of Cytokine-Based Anti-cancer Immunotherapy

Chulpanova

Kitaeva

Green

et al. 2020

Front. Cell Dev. Biol.

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Cytokine-based immunotherapy is a promising field in the cancer treatment, since cytokines, as proteins of the immune system, are able to modulate the host immune response toward cancer cell, as well as directly induce tumor cell death. Since a low dose monotherapy with some cytokines has no significant therapeutic results and a high dose treatment leads to a number of side effects caused by the pleiotropic effect of cytokines, the problem of understanding the influence of cytokines on the immune cells involved in the pro-and anti-tumor immune response remains a pressing one. Immune system cells carry CD makers on their surface which can be used to identify various populations of cells of the immune system that play different roles in pro-and anti-tumor immune responses. This review discusses the functions and specific CD markers of various immune cell populations which are reported to participate in the regulation of the immune response against the tumor. The results of research studies and clinical trials investigating the effect of cytokine therapy on the regulation of immune cell populations and their surface markers are also discussed. Current trends in the development of cancer immunotherapy, as well as the role of cytokines in combination with other therapeutic agents, are also discussed.

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Expansion of CD3+CD56+ lymphocytes correlates with induction of cytotoxicity by interleukin-2 gene transfer in human breast tumor cultures

Abstract: IL-2 gene expression by human breast cancer causes expansion of CD3+CD56+ cytotoxic-lymphocytes. This phenotype is consistent with that of a non-major histocompatibility complex (MHC)-restricted cytokine induced killer cell population previously described.

Cited by 8 publications

References 20 publications

Immunologic and other biological parameters as a function of smoking status and of residence in areas differing in terms of air pollution

Immunologic and other biological parameters as a function of smoking status and of residence in areas differing in terms of air pollution

Human Mesenchymal Stem Cells Overexpressing Interleukin 2 Can Suppress Proliferation of Neuroblastoma Cells in Co-Culture and Activate Mononuclear Cells In Vitro

Molecular Aspects and Future Perspectives of Cytokine-Based Anti-cancer Immunotherapy

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