Expansin genes expression in growing ovaries and grains of sunflower are tissue-specific and associate with final grain weight

Castillo, Francisca M.; Canales, Javier; Claude, Alejandro; Calderini, Daniel F.

doi:10.1186/s12870-018-1535-7

Cited by 16 publications

(9 citation statements)

References 84 publications

(97 reference statements)

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“… Lizana et al (2010) observed that TaExpA6 accumulates in the early grain development period, implying their role in determination of grain size in wheat. Castillo et al (2018) have shown that expansins are involved in the extension of grain tissue, and their expression is associated with the grain size of sunflower.…”

Section: Discussionmentioning

confidence: 99%

A proteomic analysis of grain yield-related traits in wheat

et al. 2020

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Grain yield, which is mainly contributed by tillering capacity as well as kernel number and weight, is the most important trait to plant breeders and agronomists. Label-free quantitative proteomics was used to analyze yield-contributing organs in wheat. These were leaf sample, tiller initiation, spike initiation, ovary, and three successive kernel development stages at five, ten, and fifteen days after anthesis. We identified 3,182 proteins across all samples. The largest number was obtained for spike initiation (1673), while the smallest was kernel sample at fifteen days after anthesis (709). Of the 3,182 proteins, 296 of them were common to all seven organs. Organ-specific proteins ranged from 148 in ovary to 561 in spike initiation. When relative protein abundances were compared to that of leaf sample, 347 and 519 proteins were identified as differentially abundant in tiller initiation and spike initiation, respectively. When compared with ovary, 81, 35, and 96 proteins were identified as differentially abundant in kernels sampled at five, ten, and fifteen days after anthesis, respectively. Our study indicated that two argonaute proteins were solely expressed in spike initiation. Of the four expansin proteins detected, three of them were mainly expressed during the first 10 days of kernel development after anthesis. We also detected cell wall invertases and sucrose and starch synthases mainly during the kernel development period. The manipulation of these proteins could lead to increases in tillers, kernels per spike, or final grain weight, and is worth exploring in future studies.

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Section: Discussionmentioning

confidence: 99%

A proteomic analysis of grain yield-related traits in wheat

et al. 2020

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“…These results are consistent with the identification of more expansins at the cellularization step, and the presence in both the endosperm and outer layers. Moreover, the role of expansins in grain is reinforced by a recent study suggesting that expansin genes could be specifically involved in grain enlargement and may control seed growth in sunflower at the early phases of development [63]. As in wheat, several DUF538 were identified in the developing grain of B. distachyon [25].…”

Section: Discussionmentioning

confidence: 92%

Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development

Cherkaoui

Lollier

Geairon

et al. 2019

IJMS

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The cell wall is an important compartment in grain cells that fulfills both structural and functional roles. It has a dynamic structure that is constantly modified during development and in response to biotic and abiotic stresses. Non-structural cell wall proteins (CWPs) are key players in the remodeling of the cell wall during events that punctuate the plant life. Here, a subcellular and quantitative proteomic approach was carried out to identify CWPs possibly involved in changes in cell wall metabolism at two key stages of wheat grain development: the end of the cellularization step and the beginning of storage accumulation. Endosperm and outer layers of wheat grain were analyzed separately as they have different origins (maternal and seed) and functions in grains. Altogether, 734 proteins with predicted signal peptides were identified (CWPs). Functional annotation of CWPs pointed out a large number of proteins potentially involved in cell wall polysaccharide remodeling. In the grain outer layers, numerous proteins involved in cutin formation or lignin polymerization were found, while an unexpected abundance of proteins annotated as plant invertase/pectin methyl esterase inhibitors were identified in the endosperm. In addition, numerous CWPs were accumulating in the endosperm at the grain filling stage, thus revealing strong metabolic activities in the cell wall during endosperm cell differentiation, while protein accumulation was more intense at the earlier stage of development in outer layers. Altogether, our work gives important information on cell wall metabolism during early grain development in both parts of the grain, namely the endosperm and outer layers. The wheat cell wall proteome is the largest cell wall proteome of a monocot species found so far.

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“…More studies on the expansin gene function had been carried out in several laboratories via gene expression (Castillo et al, 2018) and gene transformation experiment (Zenoni et al, 2011). The results really confirmed the important role of expansin genes in plant growth and development and environmental adaptation process from different perspectives.…”

Section: Discussionmentioning

confidence: 69%

Identification and Evaluation of the Expansin Genes in <i>Salix purpurea</i> Genome

Yang¹,

Liu²,

Lan³

et al. 2020

tgmb

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Expansin is an indispensable cell wall protein, involved in plant growth and resistance to stress. Based on the sequencing genome data, a total of 34 expansin genes were identified on 14 chromosomes in Salix purpurea, which were grouped into 4 subfamilies. The analysis of the physicochemical properties showed that most expansin proteins were in weakly alkaline, had a higher aliphatic index and more stable protein structure. The gene sequence was more conservative among the members in the same subfamily with the amino acid identity between 34.2% and 99.4%, while that was between 15.0% and 39.8% among the members in the different subfamilies. Also, the same subfamily genes had similar exon-intron structures, but the different subfamily genes showed that much differently. The codon usage analysis revealed that there are 5 high-frequency codons in Salix purpurea expansin genes. The codon usage bias was much affected by natural selection. The synteny analysis showed that the expansin genes divided in corresponce to whole-genome duplication events in willow. The results here would help us to understand well the role and status of expansin genes in the growth and development of Salix purpurea. It will be helpful for further study on the expansins functions.

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Expansin genes expression in growing ovaries and grains of sunflower are tissue-specific and associate with final grain weight

Cited by 16 publications

References 84 publications

A proteomic analysis of grain yield-related traits in wheat

A proteomic analysis of grain yield-related traits in wheat

Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development

Identification and Evaluation of the Expansin Genes in <i>Salix purpurea</i> Genome

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