Expansin gene TaEXPA2 positively regulates drought tolerance in transgenic wheat (Triticum aestivum L.)

Yang, Junjiao; Zhang, Guangqiang; An, Jie; Li, Qinxue; Chen, Yanhui; Zhao, Xiaoyu; Wu, Jiajie; Wang, Yong; Hao, Qunqun; Wang, Wenqiang; Wang, Wei

doi:10.1016/j.plantsci.2020.110596

Cited by 57 publications

(39 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCoEGs of GhTLP12A.1 comprised expansin and a Dof-type zinc finger in drought-stress response. Transgenic wheat expansin 2 ( TaEXPA2 ) positively regulates tolerance under drought stress (Yang et al, 2020 ), and Brassica rapa expansin-like B1 ( BrEXLB1 ) also associated with drought stress tolerance (Muthusamy et al, 2020 ), while the overexpressed DOF zinc finger family provides resistance under drought stress in P. trichocarpa (Wang H. et al, 2017 ). NCoEGs of GhTLP12A.1 contained UBA-like superfamily and dirigent protein under drought stress response ( Supplementary Table 7 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Evolutionary Analysis of Gossypium Tubby-Like Protein (TLP) Gene Family and Expression Analyses During Salt and Drought Stress

et al. 2021

View full text Add to dashboard Cite

Tubby-like proteins (TLPs) possess a highly conserved closed β barrel tubby domain at C-terminal and N-terminal F-box. The role of TLP gene family members has been widely discussed in numerous organisms; however, the detailed genome-wide study of this gene family in Gossypium species has not been reported till date. Here, we systematically identified 105 TLP gene family members in cotton (Gossypium arboreum, Gossypium raimondii, Gossypium hirsutum, and Gossypium barbadense) genomes and classified them into eight phylogenetic groups. Cotton TLP12 gene family members clustered into two groups, 4 and 8. They experienced higher evolutionary pressure in comparison to others, indicating the faster evolution in both diploid as well as in tetraploid cotton. Cotton TLP gene family members expanded mainly due to segmental duplication, while only one pair of tandem duplication was found in cotton TLPs paralogous gene pairs. Subsequent qRT-PCR validation of seven putative key candidate genes of GhTLPs indicated that GhTLP11A and GhTLP12A.1 genes were highly sensitive to salt and drought stress. The co-expression network, pathways, and cis-regulatory elements of GhTLP11A and GhTLP12A.1 genes confirmed their functional importance in salt and drought stress responses. This study proposes the significance of GhTLP11A and GhTLP12A.1 genes in exerting control over salt and drought stress responses in G. hirsutum and also provides a reference for future research, elaborating the biological roles of G. hirsutum TLPs in both stress responses.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Evolutionary Analysis of Gossypium Tubby-Like Protein (TLP) Gene Family and Expression Analyses During Salt and Drought Stress

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Moreover, expansin genes were found to be differentially expressed under different abiotic stresses (Wu et al, 2001;Bray, 2004;Harb et al, 2010;Marowa et al, 2016). The overexpression of TaEXPA2 in tobacco and wheat enhanced drought tolerance in the transgenic plants (Chen et al, 2016;Yang et al, 2020). Moreover, improved tolerance to salinity and drought was also shown in tobacco plants overexpressing the tobacco EXPA4 (Chen et al, 2018).…”

Section: A Beta-expansin Gene Was Uniquely and Highly Induced In Otismentioning

confidence: 97%

The Effect of Drought on Transcriptome and Hormonal Profiles in Barley Genotypes With Contrasting Drought Tolerance

et al. 2020

View full text Add to dashboard Cite

Like many cereal crops, barley is also negatively affected by drought stress. However, due to its simple genome as well as enhanced stress resilient nature compared to rice and wheat, barley has been considered as a model to decipher drought tolerance in cereals. In the present study, transcriptomic and hormonal profiles along with several biochemical features were compared between drought-tolerant (Otis) and drought-sensitive (Baronesse) barley genotypes subjected to drought to identify molecular and biochemical differences between the genotypes. The drought-induced decrease in the leaf relative water content, net photosynthesis, and biomass accumulation was relatively low in Otis compared to Baronesse. The hormonal profiles did not reveal significant differences for majority of the compounds other than the GA20 and the cis-zeatin-o-glucoside (c-ZOG), whose levels were greatly increased in Otis compared to Baronesse under drought. The major differences that emerged from the transcriptome analysis are; (1), the overall number of differentially expressed genes was relatively low in drought-tolerant Otis compared to drought-sensitive Baronesse; (2), a wax biosynthesis gene (CER1), and NAC transcription factors were specifically induced in Otis but not in Baronesse; (3), the degree of upregulation of betaine aldehyde dehydrogenase and a homeobox transcription factor (genes with proven roles in imparting drought tolerance), was greater in Otis compared to Baronesse; (4) the extent of downregulation of gene expression profiles for proteins of the reaction center photosystem II (PSII) (D1 and D2) was low in Otis compared to Baronesse; and, (5), alternative splicing (AS) was also found to differ between the genotypes under drought. Taken together, the overall transcriptional responses were low in drought-tolerant Otis but the genes that could confer drought tolerance were either specifically induced or greatly upregulated in the tolerant genotype and these differences could be important for drought tolerance in barley.

show abstract

“…Accumulation of ROS scavenging enzymes like superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) etc. has been reported in plants overexpressing expansin genes (18,25). The flowchart (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…A positive correlation exists between expression of expansin genes and drought tolerance in crops. A recent study showed that overexpression of TaEXPA2 in wheat helped the plants to cope up with drought stress by induced lateral root formation as well as by increased antioxidant activity (25). In another study, transgenic tobacco overexpressing TaEXPA2 improved drought tolerance in the plants (18).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, characterization and expression analysis of the expansin gene family under drought stress in tea (Camellia sinensis L.)

et al. 2021

View full text Add to dashboard Cite

During several developmental processes, expansins contribute to cell enlargement by promoting cell wall loosening. To explore the biological roles of expansins during drought stress response and to characterize different expansins in tea, we performed a detailed analysis of the expansin gene family covering phylogeny, gene structure, profiling of gene expression and co-expression network analysis. We identified a total of 40 expansin genes in the tea genome belonging to 3 subfamilies, out of which 29 tea expansins belong to EXPA, 9 to EXLA and 2 to EXPB subfamilies. A minimum of 3 and a maximum of 13 exons are present in the gene structure of expansins. Presence of drought stress responsive cis-acting elements in the upstream of promoter regions of 40% of the identified expansins shows that the putative expansins may have been involved in tea plant’s response to drought stress. At least 15 out of the 40 expansin genes are found to be differentially expressed in response to drought in each of the drought stress related public datasets analysed in-silico. TEA022767 belonging to EXPA subfamily is seen to be upregulated during drought stress, as revealed from the analysis of all three publicly available bio-projects. Co-expression network analysis shows that TEA022767 and TEA032954 form a connecting link between two expression correlation groups that further signifies their role in drought stress response in tea. This study helps to interpret and to understand the biological roles of diverse expansin genes in tea plants under drought stress conditions.

show abstract

Expansin gene TaEXPA2 positively regulates drought tolerance in transgenic wheat (Triticum aestivum L.)

Cited by 57 publications

References 74 publications

Genome-Wide Identification and Evolutionary Analysis of Gossypium Tubby-Like Protein (TLP) Gene Family and Expression Analyses During Salt and Drought Stress

Genome-Wide Identification and Evolutionary Analysis of Gossypium Tubby-Like Protein (TLP) Gene Family and Expression Analyses During Salt and Drought Stress

The Effect of Drought on Transcriptome and Hormonal Profiles in Barley Genotypes With Contrasting Drought Tolerance

Genome-wide identification, characterization and expression analysis of the expansin gene family under drought stress in tea (Camellia sinensis L.)

Contact Info

Product

Resources

About