Expanding the Morphogenetic Repertoire: Perspectives from the Drosophila Egg

Bilder, David; Haigo, Saori L.

doi:10.1016/j.devcel.2011.12.003

Cited by 75 publications

(74 citation statements)

References 82 publications

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“…7D,E). This reduction in egg length without a significant change in width is similar to other mutants that have incomplete 'dumping' of nurse cell cytoplasm into the oocyte during late oogenesis (Bilder and Haigo, 2012;Cooley et al, 1992). An examination of late stages of oogenesis indicated that the (simw 501 ); OreR females did indeed have a very high fraction of egg chambers with incomplete nurse cell dumping (Fig.…”

Section: Resultssupporting

confidence: 60%

“…8). The incomplete transfer of nurse cell cytoplasm and short egg phenotype resembles that of other 'dumpless' mutants, and suggests that mito-nuclear incompatibility also impairs germline functions (Bilder and Haigo, 2012;Frydman and Spradling, 2001;Mahajan-Miklos and Cooley, 1994). Rapid transfer of nurse cell cytoplasm depends on actin polymerization and contraction, and is sensitive to ATP/ADP ratio, perhaps explaining why it is sensitive to mitochondrial dysfunction (Huelsmann et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we investigate the impact of mito-nuclear incompatibility on Drosophila oogenesis and female fertility. Each Drosophila ovary is composed of ∼16-20 ovarioles, which contain an array of progressively more mature egg chambers (Bilder and Haigo, 2012;Lin and Spradling, 1993) (Fig. 1A,B).…”

Section: A275vmentioning

confidence: 99%

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Incompatibility between mitochondrial and nuclear genomes during oogenesis results in ovarian failure and embryonic lethality

2017

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Mitochondrial dysfunction can cause female infertility. An important unresolved issue is the extent to which incompatibility between mitochondrial and nuclear genomes contributes to female infertility. It has previously been shown that a mitochondrial haplotype from D. simulans (simw 501) is incompatible with a nuclear genome from the D. melanogaster strain Oregon-R (OreR), resulting in impaired development, which was enhanced at higher temperature. This mitonuclear incompatibility is between alleles of the nuclear-encoded mitochondrial tyrosyl-tRNA synthetase (Aatm) and the mitochondrialencoded tyrosyl-tRNA that it aminoacylates. Here, we show that this mito-nuclear incompatibility causes a severe temperature-sensitive female infertility. The OreR nuclear genome contributed to death of ovarian germline stem cells and reduced egg production, which was further enhanced by the incompatibility with simw 501 mitochondria. Mito-nuclear incompatibility also resulted in aberrant egg morphology and a maternal-effect on embryonic chromosome segregation and survival, which was completely dependent on the temperature and mito-nuclear genotype of the mother. Our findings show that maternal mito-nuclear incompatibility during Drosophila oogenesis has severe consequences for egg production and embryonic survival, with important broader relevance to human female infertility and mitochondrial replacement therapy.

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Section: Resultssupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

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Incompatibility between mitochondrial and nuclear genomes during oogenesis results in ovarian failure and embryonic lethality

2017

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“…Similar to the germline, Myo7A shows a clear association with F-actin structures in columnar follicle cells. On the basal side, where bundles of parallel actin filaments span the cell floor similar to stress fibers (reviewed by Bilder and Haigo, 2012), Myo7A appeared to be particularly concentrated at their lateral attachment points, but was also found in a dotted pattern along the actin fibers (Fig. 2D).…”

Section: Myo7a Associates With Parallel Bundles Of Actin Filaments Dumentioning

confidence: 99%

Myosin VIIA regulates microvillus morphogenesis and interacts with cadherin Cad99C in Drosophila oogenesis

et al. 2014

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BSTRACTMicrovilli and related actin-based protrusions permit multiple interactions between cells and their environment. How the shape, length and arrangement of microvilli are determined remains largely unclear. To address this issue and explore the cooperation of the two main components of a microvillus, the central F-actin bundle and the enveloping plasma membrane, we investigated the expression and function of Myosin VIIA (Myo7A), which is encoded by crinkled (ck), and its interaction with cadherin Cad99C in the microvilli of the Drosophila follicular epithelium. Myo7A is present in the microvilli and terminal web of follicle cells, and associates with several other F-actin-rich structures in the ovary. Loss of Myo7A caused brush border defects and a reduction in the amount of the microvillus regulator Cad99C. We show that Myo7A and Cad99C form a molecular complex and that the cytoplasmic tail of Cad99C recruits Myo7A to microvilli. Our data indicate that Myo7A regulates the structure and spacing of microvilli, and interacts with Cad99C in vivo. A comparison of the mutant phenotypes suggests that Myo7A and Cad99C have co-dependent and independent functions in microvilli.

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“…In particular, there has been extensive research on egg development, because oogenesis provides a tractable way to investigate many different developmental processes, including tissue patterning, cell polarity changes, cell cycle switching, and translational regulation 1,2,3,4 . One important morphogenic event during oogenesis is the specification, acquisition of motility, and migration of a set of cells called border cells (reviewed in 5 ).…”

Section: Introductionmentioning

confidence: 99%

Upright Imaging of <em>Drosophila</em> Egg Chambers

Manning

Starz‐Gaiano

2015

JoVE

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Drosophila melanogaster oogenesis provides an ideal context for studying varied developmental processes since the ovary is relatively simple in architecture, is well-characterized, and is amenable to genetic analysis. Each egg chamber consists of germ-line cells surrounded by a single epithelial layer of somatic follicle cells. Subsets of follicle cells undergo differentiation during specific stages to become several different cell types. Standard techniques primarily allow for a lateral view of egg chambers, and therefore a limited view of follicle cell organization and identity. The upright imaging protocol describes a mounting technique that enables a novel, vertical view of egg chambers with a standard confocal microscope. Samples are first mounted between two layers of glycerin jelly in a lateral (horizontal) position on a glass microscope slide. The jelly with encased egg chambers is then cut into blocks, transferred to a coverslip, and flipped to position egg chambers upright. Mounted egg chambers can be imaged on either an upright or an inverted confocal microscope. This technique enables the study of follicle cell specification, organization, molecular markers, and egg development with new detail and from a new perspective. Video LinkThe video component of this article can be found at

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Expanding the Morphogenetic Repertoire: Perspectives from the Drosophila Egg

Cited by 75 publications

References 82 publications

Incompatibility between mitochondrial and nuclear genomes during oogenesis results in ovarian failure and embryonic lethality

Incompatibility between mitochondrial and nuclear genomes during oogenesis results in ovarian failure and embryonic lethality

Myosin VIIA regulates microvillus morphogenesis and interacts with cadherin Cad99C in Drosophila oogenesis

Upright Imaging of <em>Drosophila</em> Egg Chambers

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