Expanding the Junction: New Insights into Non-Occluding Roles for Septate Junction Proteins during Development

Rice, Clinton; De, Oindrila; Alhadyian, Haifa; Hall, Sonia; Ward, Robert E.

doi:10.3390/jdb9010011

Cited by 19 publications

(17 citation statements)

References 110 publications

(245 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4C), and could not be investigated further due to cell death and tissue malformation from 3D ATS onwards. Given that Scrib complex is enriched around and functions as co-factor of SJs (Harden et al, 2016; Rice et al, 2021), we wondered whether core-components of SJs, Scrib complex and α-Cat mutually interact to sustain intercellular alignment of ABP. To corroborate this, we examined how Scrib distribution is affected by depleting α-Cat and SJs components.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Intercellular alignment of apical-basal polarity coordinates tissue homeostasis and growth

Gui

Huang

Myllymki

et al. 2021

Preprint

View full text Add to dashboard Cite

Maintaining apicobasal polarity (ABP) is crucial for epithelial integrity and homeostasis during tissue development. Although recent studies have greatly advanced our understanding of intracellular mechanisms underlying ABP establishment, it remains largely unknown how the ABP is regulated at the tissue level. Here, we address intercellular mechanisms coordinating ABP using the Drosophila wing imaginal disc. By studying Scribble, a key ABP determinant, we show that ABP is regulated through intercellular alignment, which takes place either progressively or regressively in a context-dependent manner. Cells expressing wild type scribble progressively restore ABP in scribble hypomorphic mutant cells. In contrast, cells with conditional scribble knockdown instigate the regressive loss of polarity in abutting wild type cells. Our data reveal that genetic and physical interactions between Scribble, Septate junction complex and α-Catenin appear to be key for sustaining intercellular network of ABP. Taken together, our findings indicate that the intercellular relay of the status of ABP contributes to the robustness of polarity across the tissue.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The copyright holder for this preprint this version posted October 12, 2021. ; https://doi.org/10. 1101/2021 reflected in the fact that many molecules identified as ABP determinants serve as tumor suppressors (Bilder, 2004;Hariharan, 2015).…”

Section: Introductionmentioning

confidence: 99%

Intercellular alignment of apical-basal polarity coordinates tissue homeostasis and growth

Gui

Huang

Myllymki

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…A detailed study of ultrastructural differentiation of pleated SJs in arthropod epithelia has been performed in the common fruit fly, Drosophila melanogaster by Tepass and Hartenstein (1994) . The molecular composition of SJs in the fruit fly has been characterised in several studies ( Izumi and Furuse 2014 ; Hall and Ward 2016 ; Rice et al 2021 ). The biogenesis of SJs in the hindgut epithelium of P. scaber was characterised by Bogataj et al 2019 .…”

Section: Discussionmentioning

confidence: 99%

Formation and remodelling of septate junctions in the epidermis of isopod Porcellio scaber during development

Kunčič¹,

Mrak²,

Žnidaršič³

2022

View full text Add to dashboard Cite

Septate junctions (SJs) perform an occluding function in invertebrate epithelia and consist of parallel septa extending across the intercellular space between neighbouring cells. In addition, they are required for several morphogenetic processes in arthropods. The biogenesis of SJs during development is inadequately studied and it was characterised in detail only for various epithelia of Drosophila melanogaster. This paper provides a detailed analysis of the ultrastructural differentiation of SJs in the epidermis of the terrestrial isopod Porcellio scaber during embryonic and postembryonic development. In this study, mid-stage embryo S13 was the earliest stage in which single septa were observed basally to the adherens junction (AJ). Differentiation of SJs during further development includes gradual elongation of septa arrays and formation of continuous arrays of septa. The enlargement of SJs in the epidermis is most pronounced at the transition from embryonic to postembryonic development and after the release of mancae from the marsupium. SJs of postmarsupial mancae are similar to those of adults, but are not yet as extensive. Comparison of the differentiation of SJs in the epidermis and hindgut of P. scaber, reveals a similar sequence of events. In addition, remodelling of SJs was observed in the epidermis of late marsupial mancae, the stage of cuticle renewal. Common features of SJs’ biogenesis in P. scaber and D. melanogaster ectodermal epithelia are indicated.

show abstract

“…We went on to show that loss-of-function mutations in Mcr resulted in embryonic lethality, characterized by defects in the processes of dorsal closure, head involution, and tracheal and salivary gland organogenesis ( Hall et al, 2014 ). Interestingly, Mcr localizes to the septate junction (SJ) and serves as a core component required for the organization and function of the junction ( Hall et al, 2014 ), raising the possibility that SJs or specific SJ proteins may have a role in morphogenesis (reviewed in Rice et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…The SJ consists of a large group of interdependent proteins that provide an occluding barrier to the invertebrate epithelium, similar to the function of tight junctions in vertebrates ( Lord and DiBona, 1976 ; Ward et al, 1998 ; Genova and Fehon, 2003 ). In Drosophila , more than 30 proteins have been identified as required for the establishment or maintenance of SJs ( Rice et al, 2021 ). The “core” SJ complex includes proteins from the claudin family (e.g., Megatrachea, Sinuous, Kune-Kune) ( Behr et al, 2003 ; Wu et al, 2004 ; Nelson et al, 2010 ), cell-adhesion molecules (e.g., Neurexin-IV, Neuroglian, Gliotactin, etc.)…”

Section: Introductionmentioning

confidence: 99%

Septate junction proteins are required for cell shape changes, actomyosin reorganization and cell adhesion during dorsal closure in Drosophila

Rice

Zulueta-Coarasa

et al. 2022

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Septate junctions (SJs) serve as occluding barriers in invertebrate epithelia. In Drosophila, at least 30 genes are required for the formation or maintenance of SJs. Interestingly, loss-of-function mutations in core SJ components are embryonic lethal, with defects in developmental events such as head involution and dorsal closure (DC) that occur prior to the formation of a mature SJ, indicating a role for these proteins in mid-embryogenesis independent of their occluding function. To understand this novel function in development, we examined loss-of-function mutations in three core SJ proteins during the process of DC. DC occurs during mid-embryogenesis to seal a dorsal gap in the epidermis following germ band retraction. Closure is driven by contraction of the extraembryonic amnioserosa cells that temporarily cover the dorsal surface and by cell shape changes (elongation) of lateral epidermal cells that bring the contralateral sheets together at the dorsal midline. Using live imaging and examination of fixed tissues, we show that early events in DC occur normally in SJ mutant embryos, but during later closure, coracle, Macroglobulin complement-related and Neurexin-IV mutant embryos exhibit slower rates of closure and display aberrant cells shapes in the dorsolateral epidermis, including dorsoventral length and apical surface area. SJ mutant embryos also show mild defects in actomyosin structures along the leading edge, but laser cutting experiments suggest similar tension and viscoelastic properties in SJ mutant versus wild type epidermis. In a high percentage of SJ mutant embryos, the epidermis tears free from the amnioserosa near the end of DC and live imaging and immunostaining reveal reduced levels of E-cadherin, suggesting that defective adhesion may be responsible for these tears. Supporting this notion, reducing E-cadherin by half significantly enhances the penetrance of DC defects in coracle mutant embryos.

show abstract

Expanding the Junction: New Insights into Non-Occluding Roles for Septate Junction Proteins during Development

Cited by 19 publications

References 110 publications

Intercellular alignment of apical-basal polarity coordinates tissue homeostasis and growth

Intercellular alignment of apical-basal polarity coordinates tissue homeostasis and growth

Formation and remodelling of septate junctions in the epidermis of isopod Porcellio scaber during development

Septate junction proteins are required for cell shape changes, actomyosin reorganization and cell adhesion during dorsal closure in Drosophila

Contact Info

Product

Resources

About