Expanding the genetic editing tool kit: ZFNs, TALENs, and CRISPR-Cas9

Gupta, Rajat; Musunuru, Kiran

doi:10.1172/jci72992

Cited by 402 publications

(311 citation statements)

References 95 publications

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“…Homologous recombination of exogenously supplied sequences can result in genetic modiications (knock-ins). CRISPR technology has important advantages over TALENS and ZFNs including; ease of use [164] target site selection [165] and overall eiciency, although of-target efects remains an important issue of concern [166]. CRISPR/Cas9 system may be used to alter the expression of resistance genes for constitutive expression against plant-parasitic nematodes.…”

Section: Host Resistancementioning

confidence: 99%

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

Bernard¹,

Egnin²,

Bonsi³

2017

Nematology - Concepts, Diagnosis and Control

114

101

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Plant-parasitic nematodes are costly burdens of crop production. Ubiquitous in nature, phytoparasitic nematodes are associated with nearly every important agricultural crop and represent a signiicant constraint on global food security. Root-knot nematodes (Meloidogyne spp.) cyst nematodes (Heterodera and Globodera spp.) and lesion nematodes (Pratylenchus spp.) rank at the top of list of the most economically and scientiically important species due to their intricate relationship with the host plants, wide host range, and the level of damage ensued by infection. Limitations on the use of chemical pesticides have brought increasing interest in studies on alternative methods of nematode control. Among these strategies of nonchemical nematode management is the identiication and implementation of host resistance. In addition, nematode genes involved in parasitism represent key targets for the development of control through gene silencing methods such as RNA interference. Recently, transcriptome proiling analyses has been used to distinguish nematode resistant and susceptible genotypes and identify the speciic molecular components and pathways triggered during the plant immune response to nematode invasion. This summary highlights the importance of plant-parasitic nematodes in agriculture and the molecular events involved in plant-nematode interactions.

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Section: Host Resistancementioning

confidence: 99%

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

Bernard¹,

Egnin²,

Bonsi³

2017

Nematology - Concepts, Diagnosis and Control

114

101

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“…The effect can act on the whole organism, in target tissues or cell types permanently, or under specific conditions. In recent years, the gene manipulation tools have been extended by gene editing, a protocol that targets single nucleotides or short DNA sequences and allows subtle or extreme, deleterious or advantageous modifications of DNA to study such effects on genes, proteins, or regulatory elements (Gupta and Musunuru, 2014). , 2012).…”

Section: Tools For Functional Tests Of Genesmentioning

confidence: 99%

“…This permits targeted analyses of functional domains of a gene product as well as the regulation of a gene (Esvelt et al, 2013;Gupta and Musunuru, 2014). Effective protocols that exist for gene editing use guide RNA in combination with the enzyme recombinase (CRISPR/Cas9), zinc finger nuclease, or zinc finger protein in combination with a nuclease (TALEN) (Gupta and Musunuru, 2014). Although these methods can be applied easily to livestock species, the mouse still has the unequal advantage of a short generation interval, which shortens the time for testing the effects of the introduced mutation several fold.…”

Section: Gene Editingmentioning

confidence: 99%

Invited review: Genetic and genomic mouse models for livestock research

2018

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Abstract. Knowledge about the function and functioning of single or multiple interacting genes is of the utmost significance for understanding the organism as a whole and for accurate livestock improvement through genomic selection. This includes, but is not limited to, understanding the ontogenetic and environmentally driven regulation of gene action contributing to simple and complex traits. Genetically modified mice, in which the functions of single genes are annotated; mice with reduced genetic complexity; and simplified structured populations are tools to gain fundamental knowledge of inheritance patterns and whole system genetics and genomics. In this review, we briefly describe existing mouse resources and discuss their value for fundamental and applied research in livestock.

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“…In the case of erythroid disorders, previous studies have identified GATA1 binding site mutations located in noncoding regions of a number of genes implicated in human erythroid disorders: ALAS2 in X-linked sideroblastic anemia (25,26), PKLR in pyruvate kinase deficiency (27), and UROS in congenital erythropoietic porphyria (28). These noncoding variants provide a unique opportunity to examine the necessity of these elements using modern genome editing tools, such as CRISPR/Cas9 (29). It is likely that such genome editing approaches coupled with human genetic analysis of wholegenome sequencing data, will help to identify additional noncoding variants implicated in human disease.…”

Section: Insight From Rare Disorders Of Erythropoiesismentioning

confidence: 99%

Society for Pediatric Research 2015 Young Investigator Award: genetics of human hematopoiesis—what patients can teach us about blood cell production

Wakabayashi

Sankaran

2015

Pediatr Res

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Blood cell production or hematopoiesis is one of the most well-understood paradigms of cell differentiation in the body. The majority of work on hematopoiesis comes from studies that have primarily been conducted in mice, zebrafish, or other valuable model systems. However, it is clear that such model organisms may not consistently and faithfully mimic what is observed in humans with blood disorders. Moreover, there is significant divergence between species that is increasingly being appreciated at the genomic level. As a result, there is an opportunity to use observations in humans to provide a refined view of hematopoiesis. Here, we discuss vignettes from our work that illustrate how insight from human genetics can improve our understanding of blood cell production and identify promising therapeutic approaches for blood disorders.

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Expanding the genetic editing tool kit: ZFNs, TALENs, and CRISPR-Cas9

Cited by 402 publications

References 95 publications

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

Invited review: Genetic and genomic mouse models for livestock research

Society for Pediatric Research 2015 Young Investigator Award: genetics of human hematopoiesis—what patients can teach us about blood cell production

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