2004
DOI: 10.1021/bi048364h
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Expanding the Functional Repertoire of CTD Kinase I and RNA Polymerase II:  Novel PhosphoCTD-Associating Proteins in the Yeast Proteome

Abstract: CTD kinase I (CTDK-I) of Saccharomyces cerevisiae is required for normal phosphorylation of the C-terminal repeat domain (CTD) on elongating RNA polymerase II. To elucidate cellular roles played by this kinase and the hyperphosphorylated CTD (phosphoCTD) it generates, we systematically searched yeast extracts for proteins that bound to the phosphoCTD made by CTDK-I in vitro. Initially, using a combination of far-western blotting and phosphoCTD affinity chromatography, we discovered a set of novel phosphoCTD-as… Show more

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Cited by 111 publications
(131 citation statements)
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References 115 publications
(225 reference statements)
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“…A GST-fusion protein carrying residues 1,884-2,061 of hSet2͞HYPB, containing a WW domain and a putative SRI domain, was expressed in and purified from bacterial cells (see Supporting Text, which is published as supporting information on the PNAS web site, for detailed procedures); far-Western blotting (performed as described in ref. 7) confirmed that this fusion protein binds the PCTD. Unexpectedly, thrombin cleavage released not only the intact hSet2 fragment but also a smaller piece that retained the ability to bind the PCTD (Fig.…”
Section: Dna Constructs Purification Of Recombinant Proteins and Inmentioning
confidence: 53%
See 1 more Smart Citation
“…A GST-fusion protein carrying residues 1,884-2,061 of hSet2͞HYPB, containing a WW domain and a putative SRI domain, was expressed in and purified from bacterial cells (see Supporting Text, which is published as supporting information on the PNAS web site, for detailed procedures); far-Western blotting (performed as described in ref. 7) confirmed that this fusion protein binds the PCTD. Unexpectedly, thrombin cleavage released not only the intact hSet2 fragment but also a smaller piece that retained the ability to bind the PCTD (Fig.…”
Section: Dna Constructs Purification Of Recombinant Proteins and Inmentioning
confidence: 53%
“…Attendant with changes in patterns of CTD phosphorylation, the ensemble of CTD-bound proteins changes as RNA polymerase II progresses through the transcription cycle (5). Although knowledge of the number and types of PCTD-associating proteins (PCAPs) has expanded rapidly (7,8), information about the molecular nature of PCAP-PCTD interactions remains quite limited; detailed binding properties and͞or 3D structures are known for only a few PCTD-interacting domains (PCIDs) (9)(10)(11)(12)(13)(14).…”
mentioning
confidence: 99%
“…A few of the other proteins identified in the biochemical search through the yeast proteome have already been shown to bind directly to the PCTD; these include Ess1, Prp40, Ssd1, and Hrr25 (Morris et al 1999;Morris and Greenleaf 2000;Phatnani et al 2004). Interestingly, the binding domains of three of these proteins bind best to Ser2,5P repeats, whereas the binding domain of Ssd1 binds equally well to Ser2,5P and Ser2P repeats ; the functional significance of these specificities have yet to be explored in vivo.…”
Section: Not Just Rna Processing Anymore: Many More Pcaps and Functionsmentioning
confidence: 99%
“…Finally, we point out that the order in which these interactions occur is not known; for instance, PCAPs that also bind RNA (e.g., Prp40, Cbf5) could bind the PCTD either before or after binding their cognate RNA. In addition, PCAP binding to the PCTD may be stabilized by interactions with other components of the megacomplex (e.g., Phatnani et al 2004, and its Supplementary Tables 1, 2).…”
mentioning
confidence: 99%
“…The pattern of phosphorylation on each heptad repeat and across the entire CTD is dynamic during transcription, resulting in transient and functionally distinct phospho-epitopes (3). These transient marks signal the spatio-temporal recruitment of over 100 different proteins with diverse roles (4).…”
mentioning
confidence: 99%