Expanding the Current Knowledge About the Role of Interleukin-10 to Major Concerning Bacteria

Peñaloza, Hernán F.; Noguera, Loreani P.; Riedel, Claudia A.; Bueno, Susan M.

doi:10.3389/fmicb.2018.02047

Cited by 28 publications

(22 citation statements)

References 55 publications

(95 reference statements)

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“…Cecal tonsil IL-10 mRNA content was two-fold lower in the 500μg CNP-vaccinated birds, while cecal tonsil IL-10 mRNA content was 3.3-fold higher (P<0.05) in the 1000μg CNP treatment groups, compared to that in the control group. IL-10 cytokine reduces host tissue damage in response to inflammation caused by bacterial infections [45] and hence 1000μg CNP vaccine dose was chosen for Experiment II.…”

Section: Plos Onementioning

confidence: 99%

Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

et al. 2020

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Two experiments were conducted to evaluate the immune response of broilers vaccinated with Salmonella chitosan-nanoparticle (CNP) vaccine and challenged with Salmonella. The Salmonella CNP vaccine was synthesized with Salmonella enterica outer membrane proteins (OMPs) and flagellin proteins. In Experiment I, birds were orally gavaged with PBS or 500, 1000, or 2000μg of CNP vaccine 1 and 7d-of-age. At 14d-ofage, birds were orally challenged with 1 X 10 5 CFU/bird of live S. Enteritidis (SE). Macrophage-nitrite production 11d-post-challenge was higher (P<0.05) in the 500μg group when compared to the control. At d14 (8h-post-challenge), broilers vaccinated with 1000μg CNP had higher (P<0.05) serum anti-OMPs IgG and IgA and cloacal anti-OMP IgA amounts. At 11d-post-challenge, birds vaccinated with 1000μg CNP vaccine had greater (P<0.05) bile anti-OMP and anti-flagellin IgA amounts. At 11d-post-challenge, birds administered 1000μg CNP vaccine has increased (P<0.05) IL-1β and IL-10 mRNA in cecal tonsils. In Experiment II, birds were orally gavaged with PBS or 1000μg CNP or a live commercial vaccine at 1 and 7d-of-age. At 14d-of-age, birds were orally challenged with 1 X 10 5 CFU/bird of live SE or S. Heidelberg (SH). Birds vaccinated with CNP showed higher (P<0.05) serum anti-OMPs IgG amounts at 8h-post-challenge. At 4d-post-SH challenge, birds vaccinated with CNP had higher (P<0.05) bile anti-flagellin IgA amounts. CNP decreased (P<0.05) anti-OMPs IgG levels in serum at 2d-post-SE challenge and 4d-post-SH or SE challenge. Salmonella Enteritidis loads in cecal content at 2d-post-challenge was decreased (P<0.05) by 65.9% in birds vaccinated with CNP, when compared to the control. Chitosan-nanovaccine had no adverse effects on bird's production performance. In conclusion, 1000μg CNP vaccine can induce a specific immune response against Salmonella and has the potential to mitigate SE cecal colonization in broiler birds.

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Section: Plos Onementioning

confidence: 99%

Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

et al. 2020

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“…IL-10 is produced following bacterial invasion or in stimulated tissues. IL-10 has been shown to contribute to bacterial clearance, yet minimize host damage from infection (10). Interestingly, germline mutations causing loss of function in the IL-10 receptor, as well as deficiency of the critical T reg transcription factor FOXP3, cause early-onset enterocolitis (32).…”

Section: Suppression Of the Immune Responsementioning

confidence: 99%

T Cells in Preterm Infants and the Influence of Milk Diet

et al. 2020

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Preterm infants born before 32 weeks gestational age (GA) have high rates of late onset sepsis (LOS) and necrotizing enterocolitis (NEC) despite recent improvements in infection control and nutrition. Breast milk has a clear protective effect against both these outcomes likely due to multiple mechanisms which are not fully understood but may involve effects on both the infant's immune system and the developing gut microbiota. Congregating at the interface between the mucosal barrier and the microbiota, innate and adaptive T lymphocytes (T cells) participate in this interaction but few studies have explored their development after preterm delivery. We conducted a literature review of T cell development that focuses on fetal development, postnatal maturation and the influence of milk diet. The majority of circulating T cells in the preterm infant display a naïve phenotype but are still able to initiate functional responses similar to those seen in term infants. T cells from preterm infants display a skew toward a T-helper 2(T h 2) phenotype and have an increased population of regulatory cells (T reg s). There are significant gaps in knowledge in this area, particularly in regards to innate-like T cells, but work is emerging: transcriptomics and mass cytometry are currently being used to map out T cell development, whilst microbiomic approaches may help improve understanding of events at mucosal surfaces. A rapid rise in organoid models will allow robust exploration of host-microbe interactions and may support the development of interventions that modulate T-cell responses for improved infant health.

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“…Increased blood levels of IL-6 and IL-10 have been clinically related to the high rate of mortality in patients with severe sepsis (44)(45)(46). Also, systemic levels of IL-10 appear to facilitate bacterial persistence and dissemination within the host during infections caused by intracellular bacteria or by pathogens that modulate the inflammatory responses (47,48). Indeed, increased levels of IL-10 have been linked to the progression of L. monocytogenes infections (48).…”

Section: Discussionmentioning

confidence: 99%

The TRPM2 Ion Channel Regulates Inflammatory Functions of Neutrophils During Listeria monocytogenes Infection

et al. 2020

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During infection, phagocytic cells pursue homeostasis in the host via multiple mechanisms that control microbial invasion. Neutrophils respond to infection by exerting a variety of cellular processes, including chemotaxis, activation, phagocytosis, degranulation and the generation of reactive oxygen species (ROS). Calcium (Ca 2+ ) signaling and the activation of specific Ca 2+ channels are required for most antimicrobial effector functions of neutrophils. The transient receptor potential melastatin-2 (TRPM2) cation channel has been proposed to play important roles in modulating Ca 2+ mobilization and oxidative stress in neutrophils. In the present study, we use a mouse model of Listeria monocytogenes infection to define the role of TRPM2 in the regulation of neutrophils' functions during infection. We show that the susceptibility of Trpm2 −/− mice to L. monocytogenes infection is characterized by increased migration rates of neutrophils and monocytes to the liver and spleen in the first 24 h. During the acute phase of L. monocytogenes infection, Trpm2 −/− mice developed septic shock, characterized by increased serum levels of TNF-α, IL-6, and IL-10. Furthermore, in vivo depletion of neutrophils demonstrated a critical role of these immune cells in regulating acute inflammation in Trpm2 −/− infected mice. Gene expression and inflammatory cytokine analyses of infected tissues further confirmed the hyperinflammatory profile of Trpm2 −/− neutrophils. Finally, the increased inflammatory properties of Trpm2 −/− neutrophils correlated with the dysregulated cytoplasmic concentration of Ca 2+ and potentiated membrane depolarization, in response to L. monocytogenes. In conclusion, our findings suggest that the TRPM2 channel plays critical functional roles in regulating the inflammatory properties of neutrophils and preventing tissue damage during Listeria infection.

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Expanding the Current Knowledge About the Role of Interleukin-10 to Major Concerning Bacteria

Cited by 28 publications

References 55 publications

Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

T Cells in Preterm Infants and the Influence of Milk Diet

The TRPM2 Ion Channel Regulates Inflammatory Functions of Neutrophils During Listeria monocytogenes Infection

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