Expanding Possibilities of Rolling Circle Amplification as a Biosensing Platform

DNAzymes: Selected for Applications

“…More importantly, RCA occurs isothermally, and does not require special equipment. Many excellent strategies have been reported to take full advantage of this attractive feature for the detection of a variety of targets—including DNA, RNA, DNA methylation, small molecules, proteins, and cells—which is the subject of several recent reviews . Here we will discuss a few examples where RCA is used to amplify the action of a DNAzyme.…”

“…A particular DNA‐amplification technique known as “rolling circle amplification” (RCA) has attracted great attention in the field of biosensing because of its capability of generating large amounts of DNA amplicons relatively quickly, at a constant temperature. RCA is now widely used to achieve sensitive detection of a broad selection of targets . In the final section, we will discuss representative examples of coupling activities of various DNAzymes to RCA.…”

“…RCA is now widely used to achieve sensitive detection of a broad selection of targets. [31][32][33] In the final section, we will discuss representative examples of coupling activities of various DNAzymes to RCA.…”

A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification

“…Among these methods, RCA has become increasingly popular for the quantitative detection of nucleic acids and indirect detection of other targets. [9][10][11][12][13][14][15] RCA is an isothermal enzymatic process, where a short DNA primer is amplied to form a long ssDNA in the presence of a circular DNA template and special DNA polymerases. Unlike PCR, RCA can be conducted at a constant temperature (room temperature to 37 C) in solution.…”

“…The power, simplicity, specicity and versatility of RCA technique have made it an attractive tool for biomedical research. 14,15 In the RCA-based biosensing platform, the target quanti-cation is achieved through the quantication of RCA product. The most common way to analyze RCA product is gel electrophoresis separation and radioactive-band measurements, which suffers from high labor intensity and inherent safety problems (e.g., health hazard and waste disposal problems).…”