Expanding Francisella models: Pairing up the soil amoeba Dictyostelium with aquatic Francisella

Brenz, Yannick; Winther‐Larsen, Hanne C.; Hagedorn, Monica

doi:10.1016/j.ijmm.2017.08.001

Cited by 7 publications

(6 citation statements)

References 131 publications

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“…Over the past decades, D. discoideum has emerged as a powerful model system to investigate interaction between intracellular pathogens and their host (reviewed in [1–4]). In particular, D. discoideum is commonly used as host model to study the infection routes of L. pneumophila and M. marinum , a model for M. tuberculosis .…”

Section: Discussionmentioning

confidence: 99%

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Investigation of the host transcriptional response to intracellular bacterial infection using Dictyostelium discoideum as a host model

et al. 2019

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BackgroundDuring infection by intracellular pathogens, a highly complex interplay occurs between the infected cell trying to degrade the invader and the pathogen which actively manipulates the host cell to enable survival and proliferation. Many intracellular pathogens pose important threats to human health and major efforts have been undertaken to better understand the host-pathogen interactions that eventually determine the outcome of the infection. Over the last decades, the unicellular eukaryote Dictyostelium discoideum has become an established infection model, serving as a surrogate macrophage that can be infected with a wide range of intracellular pathogens. In this study, we use high-throughput RNA-sequencing to analyze the transcriptional response of D. discoideum when infected with Mycobacterium marinum and Legionella pneumophila. The results were compared to available data from human macrophages.ResultsThe majority of the transcriptional regulation triggered by the two pathogens was found to be unique for each bacterial challenge. Hallmark transcriptional signatures were identified for each infection, e.g. induction of endosomal sorting complexes required for transport (ESCRT) and autophagy genes in response to M. marinum and inhibition of genes associated with the translation machinery and energy metabolism in response to L. pneumophila. However, a common response to the pathogenic bacteria was also identified, which was not induced by non-pathogenic food bacteria. Finally, comparison with available data sets of regulation in human monocyte derived macrophages shows that the elicited response in D. discoideum is in many aspects similar to what has been observed in human immune cells in response to Mycobacterium tuberculosis and L. pneumophila.ConclusionsOur study presents high-throughput characterization of D. discoideum transcriptional response to intracellular pathogens using RNA-seq. We demonstrate that the transcriptional response is in essence distinct to each pathogen and that in many cases, the corresponding regulation is recapitulated in human macrophages after infection by mycobacteria and L. pneumophila. This indicates that host-pathogen interactions are evolutionary conserved, derived from the early interactions between free-living phagocytic cells and bacteria. Taken together, our results strengthen the use of D. discoideum as a general infection model.

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Section: Discussionmentioning

confidence: 99%

“…For these reasons, D. discoideum has over the past decades emerged as a valuable model system to study the basic interactions between a host cell and a wide range of intracellular pathogens, e.g. Legionella pneumophila , different mycobacterial species, and Francisella noatunensis (reviewed in [2–4]).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the host transcriptional response to intracellular bacterial infection using Dictyostelium discoideum as a host model

et al. 2019

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“…While infection models against F . noatunensis have been conducted recently, no conclusive models have been available yet 104,105 . Disease challenge experiments against francisellosis using formalin killed F .…”

Section: Diseases In Farmed Atlantic Codmentioning

confidence: 99%

“…While infection models against F. noatunensis have been conducted recently, no conclusive models have been available yet. 104,105 Disease challenge experiments against francisellosis using formalin killed F. noatunensis subsp. orientalis (Fno) for immunization in tilapia (Oreochromis niloticus) showed improved survival and lower loads of bacteria in blood and internal organs.…”

Section: Bacterial Diseasesmentioning

confidence: 99%

Development of cod farming in Norway: Past and current biological and market status and future prospects and directions

Puvanendran

Mortensen

Johansen

et al. 2021

Reviews in Aquaculture

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Atlantic cod is a historically abundant species in the North Atlantic region and has contributed to the prosperity of many nations. But a decline in stocks in the last century has prompted to initiate commercial farming of cod in captive conditions. Several approaches have been employed ranging from stock enhancement, capture‐based aquaculture and intensive cod farming. However, except for the enhancement efforts which were carried out for almost a century, efforts on other methods were intermittent coinciding with lower quotas. Intensive farming was attempted in Norway, Scotland, Ireland, Canada, Iceland and Faroe Islands in the 2000s. But it was carried out hastily to cash in the demand for cod in the market even though there were many biological knowledge gaps that are required for a successful aquaculture venture. The reasons for the failure of commercial farming in Norway during the 2000s were not only because of limited knowledge of the biology of cod but also the economic meltdown in Europe in 2008. Cod farming came to a halt; however, the Norwegian National Cod Breeding Program (NCBP) initiated in 2003 continued to operate and produced a fifth generation of a domesticated cod in 2019. Efforts to fill the gaps and the selective breeding for better growth and disease resistance within NCBP have improved the quality of the juveniles produced. We will discuss the past efforts and reasons for failure in farming of cod, how the current situation looks and the future direction in terms of cod biology, political atmosphere and market.

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“…However, due to limited tools and antigens to study intracellular lifestyle in free-living amoeba, such as A. castellanii and H. vermiformis, D. discoideum has been used as a model organism to study phagocytosis, cell motility, and virulence factors for many bacterial pathogens, such as Pseudomonas, Legionella, Mycobacterium, Salmonella, and Klebsiella [18][19][20][21][22][23]. Dictyostelium has been established as a model organism for studying the life cycle of the fish pathogen, F. noatunensis, but it has not been established for studying the strains of Francisella that cause the disease in humans [24][25][26]. It has been shown that Salmonella requires O-antigen and the type VI secretion system (T6SS) for the survival within amoebae [22].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum

Kelava

Marečić

Fućak

et al. 2020

BioMed Research International

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The amoeba Dictyostelium discoideum has been used as a model organism to study host-pathogen interaction in many intracellular bacteria. Francisella tularensis is a Gram-negative, highly infectious bacterium that causes the zoonotic disease tularemia. The bacterium is able to replicate in different phagocytic and nonphagocytic cells including mammalian, amoebae, and arthropod cells. The aim of this study was to determine the optimal temperature and infection dose in the interaction of Francisella novicida with D. discoideum in order to establish a model of Francisella infection in the social amoeba. The amoeba cells were infected with a different multiplicity of infection (5, 10, and 100) and incubated at different temperatures (22, 25, 27, 30, and 37°C). The number of intracellular bacteria within D. discoideum, as well as cytotoxicity, was determined at 2, 4, 24, 48, and 72 hours after infection. Our results showed that the optimal temperature for Francisella intracellular replication within amoeba is 30°C with the MOI of 10. We can conclude that this MOI and temperature induced the optimal growth of bacteria in Dictyostelium with low cytotoxicity.

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Expanding Francisella models: Pairing up the soil amoeba Dictyostelium with aquatic Francisella

Cited by 7 publications

References 131 publications

Investigation of the host transcriptional response to intracellular bacterial infection using Dictyostelium discoideum as a host model

Investigation of the host transcriptional response to intracellular bacterial infection using Dictyostelium discoideum as a host model

Development of cod farming in Norway: Past and current biological and market status and future prospects and directions

Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum

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