Expanded Genetic Map of Gibberella moniliformis ( Fusarium verticillioides )

Abstract

Gibberella moniliformis (Fusarium verticillioides) is primarily a pathogen of maize, but it can also cause disease in other crop species. This pathogenicity, as well as the contamination of food-and feedstuffs with the fumonisin mycotoxins, results in economically significant losses to both farmers and food processors. The dissection of important biological characters in this fungus has been hampered by the lack of a uniformly dense genetic map. The existing restriction fragment length polymorphism-based map contains significant gaps, making it difficult to routinely locate biologically important genes, such as those involved in pathogenicity or mycotoxin production, with precision. We utilized amplified fragment length polymorphisms (AFLPs) to saturate the existing genetic map and added 486 AFLP markers to the ϳ150 markers on the existing map. The resulting map has an average marker interval of 3.9 map units and averages ϳ21 kb/map unit. The additional markers expanded the map from 1,452 to 2,188 map units distributed across 12 chromosomes. The maximum distance between adjacent markers is 29 map units. We identified AFLP markers less than 1 map unit from the mating type (MAT) locus and 2.5 map units from the spore killer (SK) locus; eight AFLP markers map within 8.5 units of the FUM1 (fumonisin biosynthetic) locus. The increased saturation of this map will facilitate further development of G. moniliformis as a model system for the genetic and population genetic studies of related, but less genetically tractable, plant pathogenic fungi.Gibberella moniliformis Wineland [anamorph Fusarium verticillioides (Sacc.) Nirenberg] is genetically the most intensively studied species in Fusarium section Liseola. A number of morphological and physiological mutants have been characterized in addition to anonymous restriction fragment length polymorphism (RFLP) markers (3,23,25,37,38,47

Complete genetic linkage maps from an interspecific cross between Fusarium circinatum and Fusarium subglutinans

“…It has been shown previously that the addition of markers expands the length of linkage groups (Jurgenson et al, 2002b), which could be due to possible scoring error (Hackett and Broadfoot, 2003). A lower threshold was used in constructing the parental framework maps so that all markers present in the F 1 map could also be placed in the parental maps.…”

“…Genetic maps have been published for other Fusarium species ( [Xu and Leslie, 1996], [Jurgenson et al, 2002a], [Jurgenson et al, 2002b] and [Gale et al, 2005]). The genetic map of F. verticillioides had a total length of 1452 cM and a physical distance per unit of recombination of 32 kb/cM (Xu and Leslie, 1996).…”

“…The genetic map of F. verticillioides had a total length of 1452 cM and a physical distance per unit of recombination of 32 kb/cM (Xu and Leslie, 1996). Addition of AFLP markers to the existing RFLP map increased the map length to 2188 cM and the physical distance per unit of recombination decreased to 21 kb/cM (Jurgenson et al, 2002b). The genetic map of F. graminearum had a map length of 1286 cM (Jurgenson et al, 2002a).…”

“…Genetic linkage maps have also been used to study other fungi including Fusarium verticillioides ([Xu and Leslie, 1996] and [Jurgenson et al, 2002b]) and Fusarium graminearum ([Jurgenson et al, 2002a] and [Gale et al, 2005]). In general, AFLP markers are preferred when generating linkage maps.…”

Utilização de marcador molecular SCAR na identificação de Fusarium subglutinans, agente causal da malformação da mangueira

“…Entre as técnicas moleculares utilizadas para discriminação de Fusarium spp. destacam-se: Polimorfismo de DNA amplificado ao acaso (RAPD) (Duteau & Leslie, 1991;Ouellet & Seifert, 1993;Voigt et al, 1995;Viljoen et al, 1997), Polimorfismo no comprimento de fragmentos de restrição (RFLP) (Correll et al, 1992;Nicholson et al, 1993), espaços intergênicos transcritos (ITS 1 e ITS 2) (O'Donnell & Cigelnik, 1997;Waalwijk et al, 1996) e dados de seqüências gênicas conservadas (Steenkamp et al, 2000a), Polimorfismo no comprimento de fragmentos amplificados (AFLP) (Jurgenson et al, 2002) e Reação de Polimerase em Cadeia (PCR) (Hue et al, 1999;Steenkamp et al, 2000b). Steenpkamp et al (1999) combinaram as técnicas de PCR-RFLP para a diferenciação dos fungos de F. subglutinans associados a vários hospedeiros (cana-de-açúcar, pinheiro, mangueira e milho).…”