We propose a method to construct G 2 -instantons over a compact twisted connected sum G 2 -manifold, applying a gluing result of Sá Earp and Walpuski to instantons over a pair of 7-manifolds with a tubular end. In our example, the moduli spaces of the ingredient instantons are non-trivial, and their images in the moduli space over the asymptotic cross-section K3 surface intersect transversely. Such a pair of asymptotically stable holomorphic bundles is obtained using a twisted version of the Hartshorne-Serre construction, which can be adapted to produce other examples. Moreover, their deformation theory and asymptotic behaviour are explicitly understood, results which may be of independent interest.