Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity

Yan, Litao; Wu, Xing

doi:10.1007/s10565-019-09504-5

Cited by 145 publications

(170 citation statements)

References 30 publications

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“…222 Yan et al revealed that exosomes from umbilical MSCs (U-MSC-Exos) had potent chondroprotective effects, including stimulating chondrocyte proliferation and migration, increasing matrix synthesis and inhibiting chondrocyte apoptosis. 223 Intra-articular injection of U-MSC-Exos could significantly promote the repair of cartilage defects in vivo. 223 Moreover, their data also showed that the U-MSC-Exos produced from 3D culture exerted a stronger effect on cartilage repair than those from conventional 2D culture.…”

Section: Ultrafiltrationmentioning

confidence: 99%

“…223 Intra-articular injection of U-MSC-Exos could significantly promote the repair of cartilage defects in vivo. 223 Moreover, their data also showed that the U-MSC-Exos produced from 3D culture exerted a stronger effect on cartilage repair than those from conventional 2D culture. 223 In addition, the exosomes of stem cells from human exfoliated deciduous teeth (SHEDs) have been revealed to inhibit inflammatory reactions and maintain anabolism homeostasis in temporomandibular joint chondrocytes via miR-100-5p-mediated mTOR inhibition.…”

Section: Ultrafiltrationmentioning

confidence: 99%

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Exosomes: roles and therapeutic potential in osteoarthritis

Ni¹,

et al. 2020

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Exosomes participate in many physiological and pathological processes by regulating cell-cell communication, which are involved in numerous diseases, including osteoarthritis (OA). Exosomes are detectable in the human articular cavity and were observed to change with OA progression. Several joint cells, including chondrocytes, synovial fibroblasts, osteoblasts, and tenocytes, can produce and secrete exosomes that influence the biological effects of targeted cells. In addition, exosomes from stem cells can protect the OA joint from damage by promoting cartilage repair, inhibiting synovitis, and mediating subchondral bone remodeling. This review summarizes the roles and therapeutic potential of exosomes in OA and discusses the perspectives and challenges related to exosome-based treatment for OA patients in the future.

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Section: Ultrafiltrationmentioning

confidence: 99%

Section: Ultrafiltrationmentioning

confidence: 99%

Exosomes: roles and therapeutic potential in osteoarthritis

Ni¹,

et al. 2020

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“…This suggests that MSCs may not directly differentiate into chondrocytes to participate in tissue repair in vivo, but promote cartilage regeneration through other mechanisms. In recent years, more and more researchers believed that exosomes secreted by MSCs played an important role in cartilage repair and regeneration (Marote et al, 2016;Yan and Wu, 2019;Jin et al, 2020;Liu et al, 2020). Exosomes are generally considered as communication vectors between cells, and carry a large number of complex nucleic acids (mRNA and miRNA lncRNA), proteins and lipids that can regulate and restore extracellular matrix (ECM) homeostasis (Colombo et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

The Use of Peripheral Blood-Derived Stem Cells for Cartilage Repair and Regeneration In Vivo: A Review

et al. 2020

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Background: Peripheral blood (PB) is a potential source of chondrogenic progenitor cells that can be used for cartilage repair and regeneration. However, the cell types, isolation and implantation methods, seeding dosage, ultimate therapeutic effect, and in vivo safety remain unclear. Methods: PubMed, Embase, and the Web of Science databases were systematically searched for relevant reports published from January 1990 to December 2019. Original articles that used PB as a source of stem cells to repair cartilage in vivo were selected for analysis. Results: A total of 18 studies were included. Eight human studies used autologous nonculture-expanded PB-derived stem cells (PBSCs) as seed cells with the blood cell separation isolation method, and 10 animal studies used autologous, allogenic or xenogeneic culture-expanded PB-derived mesenchymal stem cells (PB-MSCs), or nonculture-expanded PBSCs as seed cells. Four human and three animal studies surgically implanted cells, while the remaining studies implanted cells by single or repeated intra-articular injections. 121 of 130 patients (in 8 human clinical studies), and 230 of 278 animals (in 6 veterinary clinical studies) using PBSCs for cartilage repair achieved significant clinical improvement. All reviewed articles indicated that using PB as a source of seed cells enhances cartilage repair in vivo without serious adverse events. Conclusion: Autologous nonculture-expanded PBSCs are currently the most commonly used cells among all stem cell types derived from PB. Allogeneic, autologous, and xenogeneic PB-MSCs are more widely used in animal studies and are potential seed cell types for future applications. Improving the mobilization and purification technology, and shortening the culture cycle of culture-expanded PB-MSCs will obviously promote the researchers' interest. The use of PBSCs for cartilage repair and regeneration in vivo are safe. PBSCs considerably warrant further investigations due to their superiority and safety in clinical settings and positive effects despite limited evidence in humans.

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“…In addition to enriching EVs with specific miRNA as summarised above, other strategies have been used to augment the potency of MSC-EVs in cartilage repair. For instance, three-dimensional culture of UCMSCs in a hollow-fibre bioreactor resulted in a higher yield of EVs and superior therapeutic efficacy in a rabbit cartilage defect model, compared to MSC-EVs from conventional 2D cultures [106]. To ensure that MSC-EVs are retained at the site cartilage injury, human iPSC-EVs have been incorporated with in situ hydrogel glue.…”

Section: Cartilage Regenerationmentioning

confidence: 99%

Mesenchymal Stem Cell Derived Extracellular Vesicles for Tissue Engineering and Regenerative Medicine Applications

Tsiapalis

O’Driscoll

2020

Cells

212

164

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Mesenchymal stem cells (MSCs) are being extensively investigated for their potential in tissue engineering and regenerative medicine. However, recent evidence suggests that the beneficial effects of MSCs may be manifest by their released extracellular vesicles (EVs); typically not requiring the administration of MSCs. This evidence, predominantly from pre-clinical in vitro and in vivo studies, suggests that MSC-EVs may exhibit substantial therapeutic properties in many pathophysiological conditions, potentially restoring an extensive range of damaged or diseased tissues and organs. These benefits of MSC EVs are apparently found, regardless of the anatomical or body fluid origin of the MSCs (and include e.g., bone marrow, adipose tissue, umbilical cord, urine, etc). Furthermore, early indications suggest that the favourable effects of MSC-EVs could be further enhanced by modifying the way in which the donor MSCs are cultured (for example, in hypoxic compared to normoxic conditions, in 3D compared to 2D culture formats) and/or if the EVs are subsequently bio-engineered (for example, loaded with specific cargo). So far, few human clinical trials of MSC-EVs have been conducted and questions remain unanswered on whether the heterogeneous population of EVs is beneficial or some specific sub-populations, how best we can culture and scale-up MSC-EV production and isolation for clinical utility, and in what format they should be administered. However, as reviewed here, there is now substantial evidence supporting the use of MSC-EVs in tissue engineering and regenerative medicine and further research to establish how best to exploit this approach for societal and economic benefit is warranted.

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Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity

Cited by 145 publications

References 30 publications

Exosomes: roles and therapeutic potential in osteoarthritis

Exosomes: roles and therapeutic potential in osteoarthritis

The Use of Peripheral Blood-Derived Stem Cells for Cartilage Repair and Regeneration In Vivo: A Review

Mesenchymal Stem Cell Derived Extracellular Vesicles for Tissue Engineering and Regenerative Medicine Applications

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