Exosomes: New Tuberculosis Biomarkers – Prospects From the Bench to the Clinic

Kruh-Garcia, Nicole A.; Schorey, Jeffrey S.; Dobos, Karen M.

doi:10.5772/30720

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…Exosome biomarkers are used as potential diagnostic biomarkers, especially in infectious diseases. Analysis of sera from patients with active TB identified high levels of exosome concentration, compared with the control sera (over 30 times higher) (Kruh-Garcia, Schorey, & Dobos, 2012). This characteristic may be associated with the progression of TB infection and act as a diagnostic tool.…”

Section: Exosomal Proteins For Vaccination and Diagnosis Of Mycobacmentioning

confidence: 99%

Exosomes in tuberculosis: Still terra incognita?

Hadifar

Fateh

Yousefi

et al. 2018

Journal Cellular Physiology

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Today, diagnosis, vaccination, and treatment of tuberculosis (TB) remain major clinical challenges. Therefore, an introduction of new diagnostic measures and biomarkers is necessary to improve infection control. The ideal biomarker for TB infection can be defined as a host or pathogen‐derived biomolecule, which is potent for identifying infection and determining its clinical stage. Exosomes, defined as cell‐derived nanovesicles released into biological fluids, are involved in cell–cell communication and immune modulation. These vesicles have emerged as a new platform for improving the clinical diagnosis and prognosis of different infectious diseases and cancers. The role of these nanovehicles, as alternative biomarkers for the improvement of TB diagnosis and treatment, has been demonstrated in a significant body of literature. In this review, we summarized recent progress in the clinical application of exosome‐based biomarkers in TB infection.

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Section: Exosomal Proteins For Vaccination and Diagnosis Of Mycobacmentioning

confidence: 99%

Exosomes in tuberculosis: Still terra incognita?

Hadifar

Fateh

Yousefi

et al. 2018

Journal Cellular Physiology

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“…Although the potential mechanism by which mycobacterial components are localized from multivesicular bodies (MVBs) into exosomes is not known, the isolation and enrichment of exosomes can lead to the identification of potential biomarkers for TB diagnosis. A study identified more than 250 potential biomarkers from exosomes derived and purified from Mtb -infected macrophages [ 59 ]. Moreover, biomarkers have been found in biofluids isolated from Mtb -infected animal models [ 60 ].…”

Section: Classification Of Biomarkersmentioning

confidence: 99%

Exploring the Potential of Exosomes as Biomarkers in Tuberculosis and Other Diseases

Arya,

Jit,

Kumar

et al. 2024

IJMS

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Tuberculosis (TB) is a major cause of morbidity and mortality and remains an important public health issue in developing countries worldwide. The existing methods and techniques available for the diagnosis of TB are based on combinations of laboratory (chemical and biological), radiological, and clinical tests. These methods are sophisticated and laborious and have limitations in terms of sensitivity, specificity, and accuracy. Clinical settings need improved diagnostic biomarkers to accurately detect biological changes due to pathogen invasion and pharmacological responses. Exosomes are membrane-bound vesicles and mediators of intercellular signaling processes that play a significant role in the pathogenesis of various diseases, such as tuberculosis, and can act as promising biomarkers for the monitoring of TB infection. Compared to conventional biomarkers, exosome-derived biomarkers are advantageous because they are easier to detect in different biofluids, are more sensitive and specific, and may be useful in tracking patients’ reactions to therapy. This review provides insights into the types of biomarkers, methods of exosome isolation, and roles of the cargo (proteins) present in exosomes isolated from patients through omics studies, such as proteomics. These findings will aid in developing new prognostic and diagnostic biomarkers and could lead to the identification of new therapeutic targets in the clinical setting.

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“…Considering their stability in body fluids [59], and their capacity of carrying various microbial components [128,129] acting on target cells (macrophages) or tissues, exosomes provide a great deal of information about the physiological and pathological status of the originating cell via accessing their molecular contents. Exosomal proteins and miRNAs could be a novel therapeutic and diagnostic targets for pulmonary diseases [128]. Analyzing the exosomal proteins and circulating miRNAs profiles could provide information for the lung diseases pathogenesis.…”

Section: Exosome and Other Lung Diseasesmentioning

confidence: 99%

Composition and Clinical Significance of Exosomes in Tuberculosis: A Systematic Literature Review

Biadglegne

König

Rodloff

et al. 2021

JCM

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Tuberculosis (TB) remains a major health issue worldwide. In order to contain TB infections, improved vaccines as well as accurate and reliable diagnostic tools are desirable. Exosomes are employed for the diagnosis of various diseases. At present, research on exosomes in TB is still at the preliminary stage. Recent studies have described isolation and characterization of Mycobacterium tuberculosis (Mtb) derived exosomes in vivo and in vitro. Mtb-derived exosomes (Mtbexo) may be critical for TB pathogenesis by delivering mycobacterial-derived components to the recipient cells. Proteomic and transcriptomic analysis of Mtbexo have revealed a variety of proteins and miRNA, which are utilized by the TB bacteria for pathogenesis. Exosomes have been isolated in body fluids, are amenable for fast detection, and could contribute as diagnostic or prognostic biomarker to disease control. Extraction of exosomes from biological fluids is essential for the exosome research and requires careful standardization for TB. In this review, we summarized the different studies on Mtbexo molecules, including protein and miRNA and the methods used to detect exosomes in biological fluids and cell culture supernatants. Thus, the detection of Mtbexo molecules in biological fluids may have a potential to expedite the diagnosis of TB infection. Moreover, the analysis of Mtbexo may generate new aspects in vaccine development.

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Exosomes: New Tuberculosis Biomarkers – Prospects From the Bench to the Clinic

Cited by 5 publications

References 43 publications

Exosomes in tuberculosis: Still terra incognita?

Exosomes in tuberculosis: Still terra incognita?

Exploring the Potential of Exosomes as Biomarkers in Tuberculosis and Other Diseases

Composition and Clinical Significance of Exosomes in Tuberculosis: A Systematic Literature Review

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