Exosomes in the phloem and xylem of woody plants

Чухчин, Д. Г.; Bolotova, Ksenia; Sinelnikov, Igor; Churilov, D.G.; Новожилов, Е. В.

doi:10.1007/s00425-019-03315-y

Cited by 32 publications

(23 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Woody plants were subjected to cryomechanical destruction and freeze‐drying (Chukhchin et al., 2020). Split blocks of stem and branch were observed using SEM and AFM.…”

Section: Sources Of Plant Evsmentioning

confidence: 99%

In situ and ex situ imaging of plant extracellular vesicles as nanovectors for cross‐domain communication

Kim

2021

Journal of Phytopathology

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are nanometric, lipid membrane‐bound vesicles released outside the cell. Plants have not been traditionally regarded to have EVs due to the presence of a cell wall. However, since the mid‐1960s, EVs have been reported in various plant species. They are often shown as vesicles in multivesicular bodies (MVBs) that later fuse with the plasma membrane (PM). Plant EVs have been commonly visualized using electron microscopy. Using membrane‐spanning domains, plant EVs can be fluorescence‐detected. They were observed near the infection sites in host tissues attacked by pathogens or at the interfaces with symbiotic fungi. Plant EVs could be taken up by bacteria and fungi. Proteins, lipids and nucleic acids, including small ribonucleic acids (RNAs), were found in plant EVs. Plant EVs have been isolated from the symplast and apoplast of various organs and purified after density gradient ultracentrifugation. Improved protocols and technologies for EV visualization provide a basis for the future applications of EVs in plant pathology.

show abstract

“…Woody plants were subjected to cryomechanical destruction and freeze‐drying (Chukhchin et al., 2020). Split blocks of stem and branch were observed using SEM and AFM.…”

Section: Sources Of Plant Evsmentioning

confidence: 99%

In situ and ex situ imaging of plant extracellular vesicles as nanovectors for cross‐domain communication

Kim

2021

Journal of Phytopathology

View full text Add to dashboard Cite

show abstract

“…In fact, these nanovesicles have evolved in plants as a way to communicate between both plant cells and other plants or animals [ 12 , 16 ]. Nanovesicles were found both in the xylem and phloem of woody plants, supporting their role in the storage and transport of endoglucanases [ 19 ], essential for plant growth [ 20 ]. Further studies have supported the role of PDNVs in the modulation of first-line innate responses when plants are attacked by pathogens [ 11 , 16 , 17 , 18 ], by also transferring non-coding RNAs to pathogens [ 12 , 16 ] and in regulating gene expression over long distances [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Nanovesicles from Organic Agriculture-Derived Fruits and Vegetables: Characterization and Functional Antioxidant Content

Logozzi

Raimo

Mizzoni

et al. 2021

IJMS

View full text Add to dashboard Cite

Dietary consumption of fruits and vegetables is related to a risk reduction in a series of leading human diseases, probably due to the plants’ antioxidant content. Plant-derived nanovesicles (PDNVs) have been recently receiving great attention regarding their natural ability to deliver several active biomolecules and antioxidants. To investigate the presence of active antioxidants in fruits, we preliminarily analyzed the differences between nanovesicles from either organic or conventional agriculture-derived fruits, at equal volumes, showing a higher yield of nanovesicles with a smaller size from organic agriculture-derived fruits as compared to conventional ones. PDNVs from organic agriculture also showed a higher antioxidant level compared to nanovesicles from conventional agriculture. Using the PDNVs from fruit mixes, we found comparable levels of Total Antioxidant Capacity, Ascorbic Acid, Catalase, Glutathione and Superoxide Dismutase 1. Finally, we exposed the nanovesicle mixes to either chemical or physical lytic treatments, with no evidence of effects on the number, size and antioxidant capacity of the treated nanovesicles, thus showing a marked resistance of PDNVs to external stimuli and a high capability to preserve their content. Our study provides for the first time a series of data supporting the use of plant-derived nanovesicles in human beings’ daily supplementation, for both prevention and treatment of human diseases.

show abstract

“…The fusion of multivesicular bodies (MVBs) with a plasma membrane results in the release of small vesicles into the extracellular spaces of fungi and higher plants [ 10 , 11 , 12 ]. Extracellular vesicles (EVs) visualized on the internal layers of the cell walls of woody plants proved to be exosomes based on their diameter (65–145 nm) [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Bordered Pit Formation in Cell Walls of Spruce Tracheids

Чухчин

Vashukova

Новожилов

2021

Plants

Self Cite

View full text Add to dashboard Cite

The process of pit formation in plants still has various questions unaddressed and unknown, which opens up many interesting and new research opportunities. The aim of this work was elucidation of the mechanism for the formation of bordered pits of the spruce (Picea abies (L.) Karst.) tracheid with exosomes participation and mechanical deformation of the cell wall. Sample sections were prepared from spruce stem samples after cryomechanical destruction with liquid nitrogen. The study methods included scanning electron microscopy and enzymatic treatment. Enzymatic treatment of the elements of the bordered pit made it possible to clarify the localization of cellulose and pectin. SEM images of intermediate stages of bordered pit formation in the radial and tangential directions were obtained. An asynchronous mechanism of formation of bordered-pit pairs in tracheids is proposed. The formation of the pit pair begins from the side of the initiator cell and is associated with enzymatic hydrolysis of the secondary cell wall and subsequent mechanical deformation of the primary cell walls. Enzymatic hydrolysis of the S1 layer of the secondary cell wall is carried out by exosome-delivered endoglucanases.

show abstract

Exosomes in the phloem and xylem of woody plants

Cited by 32 publications

References 71 publications

In situ and ex situ imaging of plant extracellular vesicles as nanovectors for cross‐domain communication

In situ and ex situ imaging of plant extracellular vesicles as nanovectors for cross‐domain communication

Nanovesicles from Organic Agriculture-Derived Fruits and Vegetables: Characterization and Functional Antioxidant Content

Bordered Pit Formation in Cell Walls of Spruce Tracheids

Contact Info

Product

Resources

About