Exosomes from LPS-preconditioned bone marrow MSCs accelerated peripheral nerve regeneration via M2 macrophage polarization: Involvement of TSG-6/NF-κB/NLRP3 signaling pathway

Li, Cheng; Li, Xiaoxiao; Shi, Z.; Wu, Panfeng; Fu, Jinfei; Tang, Juyu; Qing, Liming

doi:10.1016/j.expneurol.2022.114139

Cited by 40 publications

(35 citation statements)

References 65 publications

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“…Potentiodynamic polarization curve measurement is one of the most widely used measurements in electrochemical corrosion ( Gu M. et al, 2022 ; Li et al, 2022 ), which can provide information on the corrosion rate and corrosion mechanism of bio-alloy materials under a simulated physiological environment and evaluate the bio-suitability of Bio-HEAs by chemical corrosion conditions. In the corrosion reaction, corrosion potential (E corr ), corrosion current density (I corr ), and AC impedance are essential parameters for analyzing the corrosion resistance of the material, and the relevant parameters of some Bio-HEAs are listed as shown in Table 2 .…”

Section: Biocompatibilitymentioning

confidence: 99%

“…For the study of the corrosion resistance of Bio-HEAs, the solutions commonly used to simulate the physiological environment include saline (0.9% NaCl solution), Hank's solution (with a composition of 0.137 M of NaCl, 5.4 mM of KCl, 0.25 mM of Na 2 HPO 4 , 0.44 mM of KH 2 PO 4 , 1.3 mM of CaCl 2 , 1.0 mM of MgSO 4 , and 4.2 mM of NaHCO 3 , pH = 7.4), phosphate buffer saline (PBS) and fetal bovine serum (FBS). Potentiodynamic polarization curve measurement is one of the most widely used measurements in electrochemical corrosion (Gu M. et al, 2022;Li et al, 2022), which can provide information on the corrosion rate and corrosion mechanism of bio-alloy materials under a simulated physiological environment and evaluate the bio-suitability of Bio-HEAs by chemical corrosion conditions. In the corrosion reaction, corrosion potential (E corr ), corrosion current density (I corr ), and AC impedance are essential parameters for analyzing the corrosion resistance of the material, and the relevant parameters of some Bio-HEAs are listed as shown in Table 2.…”

Section: Corrosion Resistancementioning

confidence: 99%

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Bio-high entropy alloys: Progress, challenges, and opportunities

Feng

Tang

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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With the continuous progress and development in biomedicine, metallic biomedical materials have attracted significant attention from researchers. Due to the low compatibility of traditional metal implant materials with the human body, it is urgent to develop new biomaterials with excellent mechanical properties and appropriate biocompatibility to solve the adverse reactions caused by long-term implantation. High entropy alloys (HEAs) are nearly equimolar alloys of five or more elements, with huge compositional design space and excellent mechanical properties. In contrast, biological high-entropy alloys (Bio-HEAs) are expected to be a new bio-alloy for biomedicine due to their excellent biocompatibility and tunable mechanical properties. This review summarizes the composition system of Bio-HEAs in recent years, introduces their biocompatibility and mechanical properties of human bone adaptation, and finally puts forward the following suggestions for the development direction of Bio-HEAs: to improve the theory and simulation studies of Bio-HEAs composition design, to quantify the influence of composition, process, post-treatment on the performance of Bio-HEAs, to focus on the loss of Bio-HEAs under actual service conditions, and it is hoped that the clinical application of the new medical alloy Bio-HEAs can be realized as soon as possible.

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Section: Biocompatibilitymentioning

confidence: 99%

Section: Corrosion Resistancementioning

confidence: 99%

Bio-high entropy alloys: Progress, challenges, and opportunities

Feng

Tang

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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“…The immunomodulatory functions of TSG-6 have been widely investigated. Notably, myeloid immune cells produce high levels of TSG-6 in response to inflammatory stimuli [ 54 ], whereby TSG-6 has been suggested to act in an autocrine mode on macrophages to promote their transition from inflammatory to anti-inflammatory and immunoregulatory phenotypes [ 51 , 55 , 56 , 57 ], likely through suppression of Toll-like receptor (TLR)-4/NF-κB pathways [ 25 , 27 , 56 , 58 , 59 , 60 ]. Notably, TLR2-related pathways regulate microglia polarization, whereas NF-κB and p38 are implicated in the polarization shift of microglia/macrophages occurring after ischemic stroke [ 61 , 62 , 63 , 64 , 65 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Involvement of Tumour Necrosis Factor (TNF)-α-Stimulated Gene 6 (TSG-6) in Ischemic Brain Injury Caused by Middle Cerebral Artery Occlusion in Mouse

Santo

Russa

Greco³

et al. 2023

IJMS

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The identification of novel targets to modulate the immune response triggered by cerebral ischemia is crucial to promote the development of effective stroke therapeutics. Since tumour necrosis factor (TNF)-α-stimulated gene 6 (TSG-6), a hyaluronate (HA)-binding protein, is involved in the regulation of immune and stromal cell functions in acute neurodegeneration, we aimed to characterize its involvement in ischemic stroke. Transient middle cerebral artery occlusion (1 h MCAo, followed by 6 to 48 of reperfusion) in mice resulted in a significant elevation in cerebral TSG-6 protein levels, mainly localized in neurons and myeloid cells of the lesioned hemisphere. These myeloid cells were clearly infiltrating from the blood, strongly suggesting that brain ischemia also affects TSG-6 in the periphery. Accordingly, TSG-6 mRNA expression was elevated in peripheral blood mononuclear cells (PBMCs) from patients 48 h after ischemic stroke onset, and TSG-6 protein expression was higher in the plasma of mice subjected to 1 h MCAo followed by 48 h of reperfusion. Surprisingly, plasma TSG-6 levels were reduced in the acute phase (i.e., within 24 h of reperfusion) when compared to sham-operated mice, supporting the hypothesis of a detrimental role of TSG-6 in the early reperfusion stage. Accordingly, systemic acute administration of recombinant mouse TSG-6 increased brain levels of the M2 marker Ym1, providing a significant reduction in the brain infarct volume and general neurological deficits in mice subjected to transient MCAo. These findings suggest a pivotal role of TSG-6 in ischemic stroke pathobiology and underscore the clinical relevance of further investigating the mechanisms underlying its immunoregulatory role.

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“…In turn, TSG-6 acts in an autocrine mode on macrophages to promote their transition from proinflammatory toward anti-inflammatory phenotypes ( Figure 1 ) [ 107 , 133 , 135 ]. In particular, this immunomodulatory protein inhibits the association between TLR-4 and MyD88, thus suppressing the activation of NF-κB, and prevents the expression of pro-inflammatory mediators (e.g., IL-6, TNF-α, IL-1β and inducible nitric oxide synthase, iNOS, STAT1, and STAT3), while elevating the expression of anti-inflammatory proteins (e.g., CD206, IL-4, and IL-10) [ 96 , 98 , 107 , 129 , 136 , 137 ].…”

Section: Immunomodulatory Functions Of Tsg-6 In Vitromentioning

confidence: 99%

Tumor Necrosis Factor (TNF)-α-Stimulated Gene 6 (TSG-6): A Promising Immunomodulatory Target in Acute Neurodegenerative Diseases

Russa

Santo

Lizasoaín

et al. 2023

IJMS

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Tumor necrosis factor (TNF)-α-stimulated gene 6 (TSG-6), the first soluble chemokine-binding protein to be identified in mammals, inhibits chemotaxis and transendothelial migration of neutrophils and attenuates the inflammatory response of dendritic cells, macrophages, monocytes, and T cells. This immunoregulatory protein is a pivotal mediator of the therapeutic efficacy of mesenchymal stem/stromal cells (MSC) in diverse pathological conditions, including neuroinflammation. However, TSG-6 is also constitutively expressed in some tissues, such as the brain and spinal cord, and is generally upregulated in response to inflammation in monocytes/macrophages, dendritic cells, astrocytes, vascular smooth muscle cells and fibroblasts. Due to its ability to modulate sterile inflammation, TSG-6 exerts protective effects in diverse degenerative and inflammatory diseases, including brain disorders. Emerging evidence provides insights into the potential use of TSG-6 as a peripheral diagnostic and/or prognostic biomarker, especially in the context of ischemic stroke, whereby the pathobiological relevance of this protein has also been demonstrated in patients. Thus, in this review, we will discuss the most recent data on the involvement of TSG-6 in neurodegenerative diseases, particularly focusing on relevant anti-inflammatory and immunomodulatory functions. Furthermore, we will examine evidence suggesting novel therapeutic opportunities that can be afforded by modulating TSG-6-related pathways in neuropathological contexts and, most notably, in stroke.

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Exosomes from LPS-preconditioned bone marrow MSCs accelerated peripheral nerve regeneration via M2 macrophage polarization: Involvement of TSG-6/NF-κB/NLRP3 signaling pathway

Cited by 40 publications

References 65 publications

Bio-high entropy alloys: Progress, challenges, and opportunities

Bio-high entropy alloys: Progress, challenges, and opportunities

Characterization of the Involvement of Tumour Necrosis Factor (TNF)-α-Stimulated Gene 6 (TSG-6) in Ischemic Brain Injury Caused by Middle Cerebral Artery Occlusion in Mouse

Tumor Necrosis Factor (TNF)-α-Stimulated Gene 6 (TSG-6): A Promising Immunomodulatory Target in Acute Neurodegenerative Diseases

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