Exosomes from Hair Follicle Epidermal Neural Crest Stem Cells Promote Acellular Nerve Allografts to Bridge Rat Facial Nerve Defects

Pan, Yao; Tang, Li; Dong, Siwei; Xu, Mengjie; Li, Qiong; Zhu, Guochen

doi:10.1089/scd.2022.0245

Cited by 7 publications

(4 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that ANAs could provide a good growth environment for stem cells or Schwann cells in vivo [22,38,39]. Additionally, studies have con rmed that chemokines secreted from the nerve stumps can not only recruit endogenous cells to assist nerve ber regeneration, but also affect the migration characteristics of the implanted cells [40][41][42][43].…”

Section: Discussionmentioning

confidence: 99%

“…According to our previous report, cells were identi ed by immuno uorescent cytochemical staining of Anti-SOX10 antibody (1:100, Abcam, Cambridge, UK) and Anti-Nestin antibody (1:100, Abcam, Cambridge, UK), respectively [22]. Subsequently, Cy3 Goat Anti-Rabbit IgG (H + L) (1:100, Servicebio, Hubei, China) and Cy3 Goat Anti-Mouse IgG (H + L) (1:100, Servicebio, Hubei, China) were incubated at 37℃ for 1 hour without light.…”

Section: Isolation Culture and Identi Cation Of Epi-ncscsmentioning

confidence: 99%

“…Since 2013, hair follicle epidermal neural crest stem cells (EPI-NCSCs) have been regarded as a sort of ideal seed cells due to their wide distribution, easy access, and biological properties of expressing a variety of neurotrophic factors, extracellular proteases, and vascular factors in central or peripheral nerve injury [20][21][22]. Similar to other stem cells, knowledge of the survival rate of the transplanted EPI-NCSCs and the e ciency of EPI-NCSCs differentiation into functional neuronal cells in vivo are the most important key-points related to research on neuro-regenerative medicine, and thus accelerate the clinical application of stem cell therapy [23].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Application of second near infrared fluorescence imaging to trace CelTrac1000-labeled hair follicle epidermal neural crest stem cells in repairing rat facial nerve defects

Lv,

Zhu,

Zhang

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background Tissue engineering based on stem cells has achieved satisfactory results in repairing facial nerve defects. However, the in vivo process of the transplanted cells has not been fully clear until now, although it is critical to understand the process and the underlying mechanism of regeneration for better therapeutic outcomes. Recently, second near-infrared window (NIR-II) fluorescence imaging has emerged as a rapidly evolving bio-imaging technique capable of visualizing and quantifying biological processes at the cellular level of living organisms. Methods Firstly, rat hair follicle epidermal neural crest stem cells (EPI-NCSCs) were isolated, cultured and identified by expression of SOX10 and Nestin, and then labeled with CelTrac1000. Rat acellular nerve allografts (ANAs) were prepared by chemical extraction. Secondly, 30 adult male rats were randomly and equally assigned into three groups: ANA + cells group, ANA group, and autograft group. The buccal branch of the facial nerve on right side was exposed and a 10-mm-long gap was bridged by ANA laden with CelTrac1000-labeled EPI-NCSCs, ANA laden with CelTrac1000 dye, and autologous nerve, respectively. Thirdly, CelTrac1000-labeled EPI-NCSCs were detected by NIR-II optical imaging system to visualize the behavior of the transplanted cells in vivo postoperatively. Finally, vibrissa movement, compound muscle action potentials (CMAPs) of vibrissal muscle, facial motoneurons retrotraced by Fluorogold, morphology and histology of the regenerated nerves in three groups were analyzed after surgery, respectively. Results Through 14 weeks of dynamic observation, we found that EPI-NCSCs successfully survived in the ANAs in vivo. Meanwhile, the region of the NIR-II fluorescence signals was gradually limited to be consistent with the route of the regenerative segment of the facial nerve. Furthermore, the degree of the vibrissa movement, the recovery value of the onset latency and amplitude of CMAPs, the number of Fluorogold-labeled cells, the CD31 positive area/total area, the mean gray value of S100 and β-tubulin III, the number and the diameter of the myelinated nerve fibers in the ANA group were lower than the other two groups (P < 0.05), and the other two groups had similar values (P > 0.05). Additionally, the thickness of the myelin sheaths was the thinnest in the ANA group, and the thickest in the autograft group (P< 0.05). Conclusions The migration map of local CelTrac1000-labeled EPI-NCSCs was successfully monitored by the NIR-II fluorescence imaging system when EPI-NCSCs within the ANAs were applied to treat rat facial nerve defects. Additionally, EPI-NCSCs promoted the ANAs to repair facial nerve defects in a small animal model.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Isolation Culture and Identi Cation Of Epi-ncscsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Application of second near infrared fluorescence imaging to trace CelTrac1000-labeled hair follicle epidermal neural crest stem cells in repairing rat facial nerve defects

Lv,

Zhu,

Zhang

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Currently, acellular nerve allografts are known to support nerve regeneration. In exosome therapy isolated from hair follicle epidermal neural crest stem cells combined with acellular nerve allografts to close facial nerve defects, it has been shown that each rat has an improvement in behavioural changes and electrophysiological tests and has the potential to replace autograft therapy in the clinic by facilitating the healing of this damage to the facial nerve [ 119 ]. Moreover, decellularising nerve allografts with adipose-derived mesenchymal stem cells (ADSC) and exosomes isolated from these cells has been suggested to accelerate neuronal regeneration [ 120 ].…”

Section: Stem Cell-based Evs and Nerve Regenerationmentioning

confidence: 99%

Applications of Stem Cell-Derived Extracellular Vesicles in Nerve Regeneration

Yavuz,

Mutlu,

Ahmed

et al. 2024

IJMS

View full text Add to dashboard Cite

Extracellular vesicles (EVs), including exosomes, microvesicles, and other lipid vesicles derived from cells, play a pivotal role in intercellular communication by transferring information between cells. EVs secreted by progenitor and stem cells have been associated with the therapeutic effects observed in cell-based therapies, and they also contribute to tissue regeneration following injury, such as in orthopaedic surgery cases. This review explores the involvement of EVs in nerve regeneration, their potential as drug carriers, and their significance in stem cell research and cell-free therapies. It underscores the importance of bioengineers comprehending and manipulating EV activity to optimize the efficacy of tissue engineering and regenerative therapies.

show abstract

Epidermal Neural Crest Stem Cell Conditioned Medium Enhances Spinal Cord Injury Recovery via PI3K/AKT-Mediated Neuronal Apoptosis Suppression

Ma,

Liu,

Liu

et al. 2024

Neurochem Res

View full text Add to dashboard Cite

This study aimed to assess the impact of conditioned medium from epidermal neural crest stem cells (EPI-NCSCs-CM) on functional recovery following spinal cord injury (SCI), while also exploring the involvement of the PI3K-AKT signaling pathway in regulating neuronal apoptosis. EPI-NCSCs were isolated from 10-day-old Sprague-Dawley rats and cultured for 48 h to obtain EPI-NCSC-CM. SHSY-5Y cells were subjected with H2O2 treatment to induce apoptosis. Cell viability and survival rates were evaluated using the CCK-8 assay and calcein-AM/PI staining. SCI contusion model was established in adult Sprague-Dawley rats to assess functional recovery, utilizing the Basso, Beattie and Bresnahan (BBB) scoring system, inclined test, and footprint observation. Neurological restoration after SCI was analyzed through electrophysiological recordings. Histological analysis included hematoxylin and eosin (H&E) staining and Nissl staining to evaluate tissue organization. Apoptosis and oxidative stress levels were assessed using TUNEL staining and ROS detection methods. Additionally, western blotting was performed to examine the expression of apoptotic markers and proteins related to the PI3K/AKT signaling pathway. EPI-NCSC-CM significantly facilitated functional and histological recovery in SCI rats by inhibiting neuronal apoptosis through modulation of the PI3K/AKT pathway. Administration of EPI-NCSCs-CM alleviated H2O2-induced neurotoxicity in SHSY-5Y cells in vitro. The use of LY294002, a PI3K inhibitor, underscored the crucial role of the PI3K/AKT signaling pathway in regulating neuronal apoptosis. This study contributes to the ongoing exploration of molecular pathways involved in spinal cord injury (SCI) repair, focusing on the therapeutic potential of EPI-NCSC-CM. The research findings indicate that EPI-NCSC-CM exerts a neuroprotective effect by suppressing neuronal apoptosis through activation of the PI3K/AKT pathway in SCI rats. These results highlight the promising role of EPI-NCSC-CM as a potential treatment strategy for SCI, emphasizing the significance of the PI3K/AKT pathway in mediating its beneficial effects. Graphical Abstract

show abstract

Exosomes from Hair Follicle Epidermal Neural Crest Stem Cells Promote Acellular Nerve Allografts to Bridge Rat Facial Nerve Defects

Cited by 7 publications

References 48 publications

Application of second near infrared fluorescence imaging to trace CelTrac1000-labeled hair follicle epidermal neural crest stem cells in repairing rat facial nerve defects

Application of second near infrared fluorescence imaging to trace CelTrac1000-labeled hair follicle epidermal neural crest stem cells in repairing rat facial nerve defects

Applications of Stem Cell-Derived Extracellular Vesicles in Nerve Regeneration

Epidermal Neural Crest Stem Cell Conditioned Medium Enhances Spinal Cord Injury Recovery via PI3K/AKT-Mediated Neuronal Apoptosis Suppression

Contact Info

Product

Resources

About