Exosomes from bone marrow-derived mesenchymal stem cells facilitate corneal wound healing via regulating the p44/42 MAPK pathway

Zhou, Junguo; Ding, Yuanyuan; Zhang, Yongqiang; Zheng, Dehui; Yan, Li-Feng; Guo, Mengxiang; Mao, Yani; Yang, Lihong

doi:10.1007/s00417-022-05956-4

Cited by 9 publications

(6 citation statements)

References 38 publications

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“…More investigations described indispensable circRNA roles to regulate biological processes [ 25 , 26 ]. Previous studies have confirmed that BMSC-Exos promoted the proliferation and migration of human corneal epithelial cells via activating the p44/42 MAPK pathway in vitro and also inhibited alkali burn-induced inflammation, fibrosis, and vascularization in corneal tissues in vivo [ 27 ]. The results showed that BMSC-derived exosomes or BMSC-exos promoted proliferation and migration and suppressed apoptosis in HaCaT cells damaged by H 2 O 2 via the miR-93-3p/APAF1 axis [ 28 ].…”

Section: Discussionmentioning

confidence: 92%

Exosomes derived from BMSCs enhance diabetic wound healing through circ-Snhg11 delivery

Tang,

Chen,

Zhang

et al. 2024

Diabetol Metab Syndr

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Background Exosomes (Exos) generated from bone mesenchymal stem cells (BMSCs) are elucidated to enhance cutaneous wound healing in mice models of diabetes mellitus (DM). While underlying mechanisms remain unknown. Methods Next-generation sequencing (NGS) was used to examine changes in circRNA expression levels following Exo treatment. Luciferase assays were used to determine the interactions between RNAs. Immunofluorescence staining was used to examine reactive oxygen species (ROS) in endothelial progenitor cells (EPCs) cultured in high glucose (HG) conditions. Therapeutic effects regarding Exos were also examined by immunofluorescence. Results We found that Exo treatment enhanced cutaneous wound healing significantly. NGS indicated that circ-Snhg11 was involved in Exo-mediated tissue repairing. Downregulation of circ-Snhg11 decreased Exo-mediated therapy responses during wound healing in diabetic mouse. Our luciferase reporter data confirmed that SLC7A11 and miR-144-3p were circ-Snhg11 downstream targets. miR-144-3p overexpression or SLC7A11 knockdown altered the protective effects of circ-Snhg11 upon EPCs exposed to HG conditions. Upregulation of circ-Snhg11 incremented therapy effects of Exo treatment during wound healing in DM mice through enhanced angiogenesis along with a reduction in GPX4-mediated ferroptosis. Conclusions circ-Snhg11 in BMSC-Exos enhanced SLC7A11/GPX4-mediated anti-ferroptosis signals via miR-144-3p sponging resulting in enhanced diabetic wound healing and improved angiopoiesis.

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Section: Discussionmentioning

confidence: 92%

Exosomes derived from BMSCs enhance diabetic wound healing through circ-Snhg11 delivery

Tang,

Chen,

Zhang

et al. 2024

Diabetol Metab Syndr

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“…Exosomes from bone marrow-derived MSCs promote wound healing via MAPK signaling in human corneal epithelial cells [ 122 ]. U0126, which is an inhibitor of MAPK, prevents exosomes from enhancing the proliferation and migration of human corneal epithelial cells [ 122 ]. Thus, MAPK inhibitors could suppress cancer cell proliferation by exerting a functional block on exosomes.…”

Section: Inhibitors Targeting Exosomesmentioning

confidence: 99%

Exosomes: Diagnostic and Therapeutic Implications in Cancer

Shim

Jeoung

2023

Pharmaceutics

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Exosomes are a subset of extracellular vesicles produced by all cells, and they are present in various body fluids. Exosomes play crucial roles in tumor initiation/progression, immune suppression, immune surveillance, metabolic reprogramming, angiogenesis, and the polarization of macrophages. In this work, we summarize the mechanisms of exosome biogenesis and secretion. Since exosomes may be increased in the cancer cells and body fluids of cancer patients, exosomes and exosomal contents can be used as cancer diagnostic and prognostic markers. Exosomes contain proteins, lipids, and nucleic acids. These exosomal contents can be transferred into recipient cells. Therefore, this work details the roles of exosomes and exosomal contents in intercellular communications. Since exosomes mediate cellular interactions, exosomes can be targeted for developing anticancer therapy. This review summarizes current studies on the effects of exosomal inhibitors on cancer initiation and progression. Since exosomal contents can be transferred, exosomes can be modified to deliver molecular cargo such as anticancer drugs, small interfering RNAs (siRNAs), and micro RNAs (miRNAs). Thus, we also summarize recent advances in developing exosomes as drug delivery platforms. Exosomes display low toxicity, biodegradability, and efficient tissue targeting, which make them reliable delivery vehicles. We discuss the applications and challenges of exosomes as delivery vehicles in tumors, along with the clinical values of exosomes. In this review, we aim to highlight the biogenesis, functions, and diagnostic and therapeutic implications of exosomes in cancer.

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“…Current tissue engineering research emphasizes characterizing different cell sources, such as bone marrow-derived mesenchymal SC (MSC) [13] or deceased donor limbal SC [14], as well as different types of scaffolds, either of xenogeneic origin [13] or synthetic biomaterials [15]. Native cell manipulation research focuses on gene therapy, such as SUV39H1, an epigenetic modifier that demonstrated a role in epithelial cell proliferation and wound healing [16]; along with posttranscriptional techniques, such as bone marrow-derived MSC exosomes which may inhibit inflammation, fibrosis, and vascularization [17]. Other studies concentrate on new delivery systems since viral vectors can cause toxicity [18].…”

Section: Epitheliummentioning

confidence: 99%

“…The overall graft failure rate was 28.6% with PED being the main cause, while no difference was found between groups and no rejections were reported. Interestingly, Prabhasawat et al ; along with posttranscriptional techniques, such as bone marrow-derived MSC exosomes which may inhibit inflammation, fibrosis, and vascularization [17]. Other studies concentrate on new delivery systems since viral vectors can cause toxicity [18].…”

Section: Epitheliummentioning

confidence: 99%

Recent advances in cell-based regenerative therapies for corneal disease

Kaufman

Jun

2023

Current Opinion in Ophthalmology

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Purpose of reviewWith limited access of more than half the world's population to corneal transplantation, regenerative medicine may represent a promising alternative. This review explores the main advancements achieved in cell-based therapies for corneal epithelium, stroma, and endothelium during 2021--2022. Recent findingsMultiple surgical techniques have been developed for epithelial limbal stem cell replacement. Recent studies aimed to gain greater understanding and characterization of these techniques. Though no clear superiority could be demonstrated, simple limbal epithelial transplantation seems to have the most clinical and cost effectiveness. For stromal disease, autologous adipose-derived stem cells have shown favorable results. For endothelial dysfunction, the validity of intracameral cultivated allogeneic endothelial cell injection and Descemetorrhexis without endothelial keratoplasty, as well as the benefits of adjunctive rho-associated kinase inhibitors, were emphasized. SummaryA plethora of innovative cell-based regenerative therapies for corneal diseases have been developed in past years. While recent literature solidifies our knowledge, most studies are still in preliminary or preclinical stages. Though showing great promise, these approaches will require larger studies with betterdefined endpoints to establish their benefits over currently available treatments.

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Exosomes from bone marrow-derived mesenchymal stem cells facilitate corneal wound healing via regulating the p44/42 MAPK pathway

Cited by 9 publications

References 38 publications

Exosomes derived from BMSCs enhance diabetic wound healing through circ-Snhg11 delivery

Exosomes derived from BMSCs enhance diabetic wound healing through circ-Snhg11 delivery

Exosomes: Diagnostic and Therapeutic Implications in Cancer

Recent advances in cell-based regenerative therapies for corneal disease

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