Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway

Li, Jiali; Zhao, Bohao; Dai, Yingying; Zhang, Xiyu; Chen, Yang; Wu, Xinsheng

doi:10.1155/2022/9042345

Cited by 11 publications

(9 citation statements)

References 50 publications

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“… 13 This result corroborated findings of previous in vivo studies demonstrating hair growth effects including the acceleration of the telogen‐to‐anagen transition in mice, potentially through the upregulation of fibroblast growth factor and β‐catenin pathways 7,13,20 . DPCs may function as a paracrine regulator of hair follicle stem cells (HFSCs) and ORSCs 7,13–15 . Interestingly, another in vitro study showed that exosomes isolated from human ORSCs exhibited similar effects vice versa in DPCs (Table 1).…”

Section: Resultssupporting

confidence: 87%

“…7,13,20 DPCs may function as a paracrine regulator of hair follicle stem cells (HFSCs) and ORSCs. 7,[13][14][15] Interestingly, another in vitro study showed that exosomes isolated from human ORSCs exhibited similar effects vice versa in DPCs (Table 1). 17 Other potential sources of exosomes include myeloid-derived suppressor cells and amniotic fluid stem cells (Table 1).…”

Section: Preclinical Evidencementioning

confidence: 97%

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Systematic review of exosome treatment in hair restoration: Preliminary evidence, safety, and future directions

Gupta

Wang

Rapaport³

2023

J of Cosmetic Dermatology

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BackgroundExosomes are small extracellular vesicles with potential roles in modulating the hair growth cycle and are an emerging therapy for patients with alopecia. In recent years, researchers have made significant progress in deciphering the network of cellular interactions and signaling pathways mediated by the transfer of exosomes. This has opened the door to a wide range of potential therapeutic applications with an increasing focus on its application in precision medicine.AimTo evaluate current published evidence, both preclinical and clinical, on the use of exosomes for hair restoration.MethodsIn January 2023, a systematic search was conducted using PubMed, Embase, and the Cochrane Library. Records were identified, screened, and assessed for eligibility as per the PRISMA guideline.ResultsWe identified 16 studies (15 preclinical and 1 clinical) showing varying degrees of efficacy using exosomes derived from sources including adipose‐derived stem cells (ADSCs) and dermal papilla cells (DPCs). Applications of exosomes isolated from ADSCs (ADSC‐Exo) and DPCs have shown early promising results in preclinical studies corroborated by results obtained from different model systems. Topical ADSC‐Exo has been tried successfully in 39 androgenetic alopecia patients demonstrating significant increases in hair density and thickness. No significant adverse reactions associated with exosome treatment have been reported thus far.ConclusionsAlthough current clinical evidence supporting the use of exosome treatment is limited, there is a growing body of evidence suggesting its therapeutic potential. Further studies are warranted to define its mechanism of action, optimize its delivery and efficacy, and to address important safety concerns.

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Section: Resultssupporting

confidence: 87%

Section: Preclinical Evidencementioning

confidence: 97%

Systematic review of exosome treatment in hair restoration: Preliminary evidence, safety, and future directions

Gupta

Wang

Rapaport³

2023

J of Cosmetic Dermatology

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“…DPCs and HFSCs were isolated as previously described [ 53 ]. DPCs were cultured in Mesenchymal Stem Cell Medium (ScienCell, SanDiego, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Dermal PapillaCell-Derived Exosomes Regulate Hair Follicle Stem Cell Proliferation via LEF1

Zhao

Shan

et al. 2023

IJMS

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Hair follicle (HF) growth and development are controlled by various cell types, including hair follicle stem cells (HFSCs) and dermal papilla cells (DPCs). Exosomes are nanostructures that participate in many biological processes. Accumulating evidence indicates that DPC-derived exosomes (DPC-Exos) mediate HFSC proliferation and differentiation during the cyclical growth of hair follicles. In this study, we found that DPC-Exos increase ki67 expression and CCK8 cell viability readouts in HFSCs but reduce annexin staining of apoptotic cells. RNA sequencing of DPC-Exos-treated HFSCs identified 3702 significantly differentially expressed genes (DEGs), including BMP4, LEF1, IGF1R, TGFβ3, TGFα, and KRT17. These DEGs were enriched in HF growth- and development-related pathways. We further verified the function of LEF1 and showed that overexpression of LEF1 increased the expression of HF development-related genes and proteins, enhanced HFSC proliferation, and reduced HFSC apoptosis, while knockdown of LEF1 reversed these effects. DPC-Exos could also rescue the siRNA-LEF1 effect in HFSCs. In conclusion, this study demonstrates that DPC-Exos mediated cell-to-cell communication can regulate HFSCs proliferation by stimulating LEF1 and provide novel insights into HF growth and development regulatory mechanisms.

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“…These studies provide the basis for the future use of artificial HFSCs and their exosomes for wound treatment. Additionally, dermal papilla cells-derived exosomes have been shown to promote hair follicle growth 80 - 82 . Although these studies did not clearly state that this exosome promotes wound repair, it can be speculated that it may also have a cooperative function in promoting skin cell regeneration.…”

Section: Introductionmentioning

confidence: 99%

Exosomes and Their Bioengineering Strategies in the Cutaneous Wound Healing and Related Complications: Current Knowledge and Future Perspectives

Yang¹,

Waheed²,

Shekh³

et al. 2023

Int. J. Biol. Sci.

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Exosomes, as therapeutically relevant cell-secreted extracellular vesicles, have attracted enormous interest because they participate in intercellular communication and facilitate wound healing. Stem cell-derived exosomes exhibit similar biological effects to source cells with the exception of low immunogenicity and no tumorigenicity, as well as superior efficacy in promoting wound healing. Exosomes accelerate wound healing by promoting angiogenesis and cell proliferation, as well as balancing inflammatory responses. Particularly, when exosomes are genetically modified or used in combination with materials, they can exhibit better comprehensive therapeutic properties, such as enriching active ingredients, targeted delivery, and physiological barrier to penetration, which are not available in traditional single products. Besides, exosomes have also been considered for diagnostic and therapeutic uses related to wounds, such as repairing complex wounds, enhancing graft success, treating related complications, and serving as diagnostic biomarkers. However, their clinical applications still face challenges, as reliable commercial products are not yet available. This review will focus on recent research advances that describe the characteristics and isolation of exosomes, introduce the sources of exosomes suitable for wound repair and related complications, illustrate the value of engineered exosomes and their development directions in the future, and provide evidence for the potential therapeutic application of exosomes in wound healing, as well as discuss potential risks, challenges, and solutions for future applications.

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Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway

Cited by 11 publications

References 50 publications

Systematic review of exosome treatment in hair restoration: Preliminary evidence, safety, and future directions

Systematic review of exosome treatment in hair restoration: Preliminary evidence, safety, and future directions

Dermal PapillaCell-Derived Exosomes Regulate Hair Follicle Stem Cell Proliferation via LEF1

Exosomes and Their Bioengineering Strategies in the Cutaneous Wound Healing and Related Complications: Current Knowledge and Future Perspectives

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