Exosomes derived from bone marrow mesenchymal stem cells promote osteosarcoma development by activating oncogenic autophagy

Huang, Yao; Liu, Wei; He, Bing; Wang, Lei; Zhang, Fucheng; Shu, Hao; Sun, Luning

doi:10.1016/j.jbo.2020.100280

Cited by 42 publications

(39 citation statements)

References 32 publications

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“…OS is one of the most common malignant bone tumours in adolescents (22). Several lncRNAs have been reported to be involved in the regulation of OS.…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 in vitro

Wang

Liang³

2021

Exp Ther Med

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The aim of the present study was to clarify the effect of long non-coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) on the proliferation, migration and invasion of osteosarcoma (OS) cells and to explore the potential underlying mechanisms. The expression levels of SNHG1, microRNA (miR)-424-5p and fibroblast growth factor 2 (FGF2) in OS tissues and cells were detected using reverse transcription-quantitative polymerase chain reaction. OS cell proliferation, migration and invasion were analysed by MTT, wound healing and Transwell invasion assays, respectively. The targeting relationships between SNHG1 and miR-424-5p, as well as between miR-424-5p and FGF2, were confirmed using RNA-binding protein immunoprecipitation and/or dual-luciferase reporter gene assays. The results demonstrated that the expression levels of SNHG1 and FGF2 were upregulated, whereas the expression of miR-424-5p was downregulated in OS tissues and cells. The silencing of SNHG1 significantly inhibited the proliferation, migration and invasion of OS cells. Additionally, FGF2 was shown to be a target of miR-424-5p, which in turn, was a target of SNHG1. miR-424-5p silencing and FGF2 overexpression both reversed the suppressive effects of SNHG1 knockdown on the proliferation, migration and invasion of OS cells. Thus, the silencing of SNHG1 may inhibit the proliferation, migration and invasion of OS cells by regulating the miR-424-5p/FGF2 axis.

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“…OS is one of the most common malignant bone tumours in adolescents (22). Several lncRNAs have been reported to be involved in the regulation of OS.…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 in vitro

Wang

Liang³

2021

Exp Ther Med

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“…Although MSC‐Exos can alleviate various diseases, they may also promote tumorigenesis. Huang et al 85 illustrated that human BMSC‐Exos promoted tumorigenesis and metastasis by inducing carcinogenic autophagy in osteosarcoma.…”

Section: Exosomes Derived From Mscs Mitigate Various Diseases By Regumentioning

confidence: 99%

Crosstalk between exosomes and autophagy: A review of molecular mechanisms and therapies

Xing

Tan

Miao

et al. 2021

J Cellular Molecular Medi

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Exosomes are extracellular vesicles that primarily exist in bodily fluids such as blood. Autophagy is an intracellular degradation process, which, along with exosomes, can significantly influence human health and has therefore attracted considerable attention in recent years. Exosomes have been shown to regulate the intracellular autophagic process, which, in turn, affects the circulating exosomes. However, crosstalk between exosomal and autophagic pathways is highly complex, depends primarily on the environment, and varies greatly in different diseases. In addition, studies have demonstrated that exosomes, from specific cell, can mitigate several diseases by regulating autophagy, which can also affect the excessive release of some harmful exosomes. This phenomenon lays a theoretical foundation for the improvement of many diseases. Herein, we review the mechanisms and clinical significance of the association and regulation of exosomes and autophagy, in order to provide a new perspective for the prevention and treatment of associated diseases.

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“…Although the importance of autophagy and exosomes in tumor progression is well-documented ( Yu et al, 2007 ; Clayton et al, 2008 ; Skog et al, 2008 ; Hong et al, 2009 ; Svensson et al, 2013 ; Keulers et al, 2016 ; Ahmadi and Rezaie, 2020 ; Amaravadi et al, 2020 ), in recent years the understanding of their connection and interplay in cancer has aroused a lot of interest ( Table 1 ; Kulshreshtha et al, 2007 ; Bellot et al, 2009 ; Chiavarina et al, 2010 ; Mazure and Pouysségur, 2010 ; White et al, 2010 ; Aga et al, 2014 ; Dutta et al, 2014 ; Li et al, 2016 ; Zhang et al, 2018 , 2019 ; Jin et al, 2019 ; Meng et al, 2019 ; Shao et al, 2019 ; Wang et al, 2019 , 2020 ; Yeon et al, 2019 ; Yuwen et al, 2019 ; Zeng et al, 2019 ; Zhou et al, 2019 ; Dai et al, 2020 ; Han et al, 2020 ; Huang et al, 2020 ; Kulkarni et al, 2020 ; Kumar and Deep, 2020 ; Liu et al, 2020 ; Sung et al, 2020 ; Wu X. et al, 2020 ; Yao et al, 2020 ).…”

Section: Autophagy and Exosomes Relationship In Cancermentioning

confidence: 99%

“…Recently, exosomes carrying miR-1910-3p secreted by breast cancer cells have been found to promote tumor development inducing proliferation, migration and autophagy in recipient mammary epithelial cells and breast cancer cells ( Table 1 ; Wang et al, 2020 ). Several recent studies have shown that specific exosomal miRNAs regulate autophagy-dependent therapy resistance in recipient cells ( Kaminskyy et al, 2012 ; Zhou et al, 2012 ; Dutta et al, 2014 ; Zhang et al, 2018 ; Deng et al, 2019 ; Huang et al, 2020 ; Kang et al, 2020 ). In breast cancer, exosomal miR-567 down-regulates ATG5 and consequently autophagy, reversing trastuzumab resistance ( Dutta et al, 2014 ).…”

Section: Autophagy and Exosomes Relationship In Cancermentioning

confidence: 99%

“…Other authors have begun to investigate the importance of exosomes-autophagy interplay between normal and neoplastic cells in supporting carcinogenesis. For example, mesenchymal stem cells (MSCs)-derived exosomes have been identified to modulate autophagy in pathological conditions such as during ischemia or spinal cord injury ( Baixauli et al, 2014 ; Tian et al, 2019 ) and, more recently, in cancer ( Huang et al, 2020 ). Huang et al (2020) indeed, found that MSCs-derived exosomes promote osteosarcoma development and invasion by inducing autophagy.…”

Section: Autophagy and Exosomes Relationship In Cancermentioning

confidence: 99%

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Autophagy and Exosomes Relationship in Cancer: Friends or Foes?

Colletti

Ceglie

Giannatale

et al. 2021

Front. Cell Dev. Biol.

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Autophagy is an intracellular degradation process involved in the removal of proteins and damaged organelles by the formation of a double-membrane vesicle named autophagosome and degraded through fusion with lysosomes. An intricate relationship between autophagy and the endosomal and exosomal pathways can occur at different stages with important implications for normal physiology and human diseases. Recent researches have revealed that extracellular vesicles (EVs), such as exosomes, could have a cytoprotective role by inducing intracellular autophagy; on the other hand, autophagy plays a crucial role in the biogenesis and degradation of exosomes. Although the importance of these processes in cancer is well established, their interplay in tumor is only beginning to be documented. In some tumor contexts (1) autophagy and exosome-mediated release are coordinately activated, sharing the molecular machinery and regulatory mechanisms; (2) cancer cell-released exosomes impact on autophagy in recipient cells through mechanisms yet to be determined; (3) exosome-autophagy relationship could affect drug resistance and tumor microenvironment (TME). In this review, we survey emerging discoveries relevant to the exosomes and autophagy crosstalk in the context of cancer initiation, progression and recurrence. Consequently, we discuss clinical implications by targeting autophagy-exosomal pathway interaction and how this could lay a basis for the purpose of novel cancer therapeutics.

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Exosomes derived from bone marrow mesenchymal stem cells promote osteosarcoma development by activating oncogenic autophagy

Cited by 42 publications

References 32 publications

Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 in vitro

Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 in vitro

Crosstalk between exosomes and autophagy: A review of molecular mechanisms and therapies

Autophagy and Exosomes Relationship in Cancer: Friends or Foes?

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