Exosomes Derived from Adipose Stem Cells Enhance Angiogenesis in Diabetic Wound Via miR-146a-5p/JAZF1 Axis

Abstract

Chronic trauma in diabetes is a leading cause of disability and mortality. Exosomes show promise in tissue regeneration. This study investigates the role of exosomes derived from adipose stem cells (ADSC-Exos) in angiogenesis. MiRNA-seq analysis revealed significant changes in 47 genes in human umbilical vein endothelial cells (HUVECs) treated with ADSC-Exos, with miR-146a-5p highly expressed. MiR-146a-5p mimics enhanced the pro-angiogenic effects of ADSC-Exos, while inhibitors had the opposite effect. JAZF1 was identified as a direct downstream target of miR-146a-5p through bioinformatics, qRT-PCR, and dual luciferase assay. Overexpress of JAZF1 resulted in decreased proliferation, migration, and angiogenic capacity of HUVECs, and reduced VEGFA expression. This study proposes that ADSC-Exos regulate angiogenesis partly via the miR-146a-5p/JAZF1 axis. Graphical Abstract

Adipose-Derived Stem Cell Exosomes Facilitate Diabetic Wound Healing: Mechanisms and Potential Applications

“…Angiogenesis: Che et al found that ADSC-exos upregulated miR-146a-5p in HUVECs, inhibited the expression of the downstream protein JAZF1, promoted the expression of VEGFA, and consequently facilitated angiogenesis in diabetic wounds. 105 Huang et al demonstrated that NFIC in ADSC-exos could promote miR-204-3p transcription to inhibit downstream gene HIPK2 in HUVECs, thus promoting angiogenesis. 104 Wang et al found higher circ-Astn1 expression in ADSCs than in EPCs.…”