Exosomes Decrease In Vitro Infectivity of HIV-1 Preparations: Implication for CD4+T Lymphocyte Depletion In Vivo

Subra, Caroline; Burelout, Chantal; Sophie, Proulx; Simard, Sébastien; Gilbert, Caroline

doi:10.5772/19846

Cited by 3 publications

(2 citation statements)

References 154 publications

(121 reference statements)

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“…We demonstrated in our previous studies that the exosome release from iMDDCs is increased following HIV-1 cell pulsing and that these exosomes have a deleterious effect on neighboring CD4TL (Subra et al, 2011a(Subra et al, , 2011b. However, the role of DCIR in exosome release from HIV-1-pulsed-dendritic cells remains unknown.…”

Section: Impact Of the Dcir-directed Inhibitor On Exosome Release By mentioning

confidence: 89%

Exosome release following activation of the dendritic cell immunoreceptor: A potential role in HIV-1 pathogenesis

et al. 2015

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Exosomes are extracellular vesicles (EVs) that play a role in intercellular communication. Stimulation of dendritic cells by the HIV-1 virus triggers their release. HIV-1 binds to dendritic cells via dendritic cell immunoreceptor (DCIR). This study shows that inhibiting the binding to DCIR significantly decreases exosome release by HIV-1-pulsed dendritic cells. In addition, exosome release from Raji-CD4 expressing DCIR cells stimulated by anti-DCIR or HIV-1 is decreased when the immunoreceptor tyrosine-based inhibition motif (ITIM) signaling motif of DCIR is mutated. Unlike the EVs released from Raji-CD4-DCIR cells after antibody stimulation, those released from HIV-1-infected cells contain the pro-apoptotic protein DAP-3. Furthermore, EVs from HIV-1 pulsed dendritic cells increase spontaneous apoptosis in uninfected CD4 T lymphocytes while they decrease it in neutrophils. This study describes for the first time that DCIR plays a role in the release of exosomes strengthening the importance of this receptor and EVs/exosomes in HIV-1 pathogenesis.

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Section: Impact Of the Dcir-directed Inhibitor On Exosome Release By mentioning

confidence: 89%

Exosome release following activation of the dendritic cell immunoreceptor: A potential role in HIV-1 pathogenesis

et al. 2015

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“…This difference was only significant in HIV-infected patients regardless of staining (CD45, CD8, and CD4). Several studies have shown that HIV infection induces exosome release by immune cells [ 8 , 9 , 81 , 82 ], and this could explain why CD45+, CD8+, or CD4+ EVs correlated with EV number in PLWH. Counts of EV subsets were correlated with CD8 T-cell count and CD4/CD8 ratio, and both parameters are used for clinical HIV infection management.…”

Section: Discussionmentioning

confidence: 99%

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV

Bazié

Boucher

Vitry

et al. 2021

PAI

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Background: Extracellular vesicles (EVs) are intercellular messengers with epigenetic potential since they can shuttle microRNA (miRNA). EVs and miRNA play a role in human immunodeficiency virus (HIV) infection immunopathogenesis. Chronic immune activation and systemic inflammation during HIV infection despite effective antiretroviral therapy (ART) are associated with non-acquired immunodeficiency syndrome (AIDS) comorbidities in people living with HIV (PLWH). Analysis of plasma EVs and their miRNA content may be useful as immune activation or inflammatory biomarkers in PLWH receiving ART. In this study, we hypothesized that the number, size, and miRNA of large and small EVs could reflect immune activation associated with an elevated CD8 T-cell count or a low CD4/CD8 ratio in PLWH.Methods: Plasma EVs subtype purified from PLWH and uninfected controls were sized using dynamic light scattering and quantified using flow cytometry and acetylcholine esterase (AChE) activity. Expression of mature miRNAs miR-92, miR-155, miR-223 was measured by quantitative reverse-transcriptase polymerase chain reaction in EVs and leucocytes.Results: HIV infection induces increased production of small EVs in plasma. EV subtypes were differentially enriched in miR-92, miR-155, and miR-223. Positive correlations between CD8 T-cell count and large EVs abundance and small EVs AChE activity were observed. CD4/CD8 ratio was negatively correlated with small EV AChE activity, and miRNA-155 level per small EV was negatively correlated with CD8 T-cell count.Conclusions: These findings suggest that quantifying large or small EVs and profiling miRNA content per EV might provide new functional biomarkers of immune activation and inflammation.

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Role of EVs as promotors for activation of leukemia-derived dendritic cell (DCleu)-mediated antileukemic immune response against AML-blasts

Lin,

Görgens,

Mussack

et al. 2023

Preprint

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Myeloid leukemia blasts can be converted into dendritic cells of leukemic origin (DCleu), which effectively activate and enhance immune-cells against leukemic blasts. EVs modulate a plethora of physiological and pathological activities. EVs secreted by dendritic cells (DCs) can activate T lymphocytes, displaying potential as promoters of adaptive immune responses. DC/DCleu generation of healthy donors’ (n=9) and AML patients’ (n=9) whole blood (WB) were treated with Kit M (GM-CSF and PGE1) (vs. control), T-cell enriched mixed lymphocyte culture (MLC) with treated vs un-treated WB and antileukemic functional assays were quantified via flow cytometry. Qualitative and quantitative characterization EVs from DC/MLC culture supernatants (DCS/MLCS) in healthy and AML samples were measured. Kit M significantly increased frequencies of (mature) DC/DCleu compared to control without induction of blast proliferation. Kit M increased significantly activated (leukemia-specific) cells of the adaptive and innate immune system after T cell-enriched MLC compared to control. EVs were qualitatively and quantitatively different in DCS/MLCS with Kit M treated vs untreated from healthy vs AML samples by TEM, fNTA and MBFCM. These EVs findings and correlations with clinical parameters contribute to understand the functional role of EVs in DCS/MLCS from healthy and AML samples, with respect to develop new EV biomarkers.

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Exosomes Decrease In Vitro Infectivity of HIV-1 Preparations: Implication for CD4+T Lymphocyte Depletion In Vivo

Cited by 3 publications

References 154 publications

Exosome release following activation of the dendritic cell immunoreceptor: A potential role in HIV-1 pathogenesis

Exosome release following activation of the dendritic cell immunoreceptor: A potential role in HIV-1 pathogenesis

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV

Role of EVs as promotors for activation of leukemia-derived dendritic cell (DCleu)-mediated antileukemic immune response against AML-blasts

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