Exosome trapping and enrichment using a sound wave activated nano-sieve (SWANS)

Habibi, Ruhollah; He, Vincent; Ghavamian, Sara; Marco, Alex de; Lee, Tzong Hsien; Aguilar, Marie‐Isabel; Zhu, Dandan; Lim, Rebecca; Neild, Adrian

doi:10.1039/d0lc00623h

Cited by 37 publications

(38 citation statements)

References 51 publications

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“…More recently, a SAW-based approach has also demonstrated the trapping of nanoparticles by means of scattered sound interaction with particles in a packed bed . Later, this system also reported EV isolation from cell culture supernatant at a throughput of 100 nL/min, indicating potential for significant scalability …”

Section: Introductionmentioning

confidence: 99%

“…24 Later, this system also reported EV isolation from cell culture supernatant at a throughput of 100 nL/min, indicating potential for significant scalability. 25 In this paper, we present a novel, scaled-up acoustic trapping device with significantly increased throughput and capacity as compared to previously reported systems. The new device comprises a piezoelectric transducer and a glass capillary with a cross-sectional area 20 times larger than the capillary in the acoustic trapping system in the aforementioned studies.…”

Section: ■ Introductionmentioning

confidence: 99%

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Multinodal Acoustic Trapping Enables High Capacity and High Throughput Enrichment of Extracellular Vesicles and Microparticles in miRNA and MS Proteomics Studies

et al. 2021

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We report a new design of an acoustophoretic trapping device with significantly increased capacity and throughput, compared to current commercial acoustic trapping systems. Acoustic trapping enables nanoparticle and extracellular vesicle (EV) enrichment without ultracentrifugation. Current commercial acoustic trapping technology uses an acoustic single-node resonance and typically operates at flow rates <50 μL/min, which limits the processing of the larger samples. Here, we use a larger capillary that supports an acoustic multinode resonance, which increased the seed particle capacity 40 times and throughput 25–40 times compared to single-node systems. The resulting increase in capacity and throughput was demonstrated by isolation of nanogram amounts of microRNA from acoustically trapped urinary EVs within 10 min. Additionally, the improved trapping performance enabled isolation of extracellular vesicles for downstream mass spectrometry analysis. This was demonstrated by the differential protein abundance profiling of urine samples (1–3 mL), derived from the non-trapped versus trapped urine samples.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Multinodal Acoustic Trapping Enables High Capacity and High Throughput Enrichment of Extracellular Vesicles and Microparticles in miRNA and MS Proteomics Studies

et al. 2021

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“…Assisted trapping on a packed microcolumn was reported by Habibi et al. [39]. Their sound wave‐activated nano‐sieve consisted of 7 to 15 μm polymer seed particles packed into the PDMS microchannel, being held in the channel by a row of micropillars.…”

Section: Applicationsmentioning

confidence: 99%

Acoustofluidic platforms for particle manipulation

2021

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There is an increasing interest in acoustics for microfluidic applications. This field, commonly known as acoustofluidics involves the interaction of ultrasonic standing waves with fluids and dispersed microparticles. The combination of microfluidics and the so-called acoustic standing waves (ASWs) led to the development of integrated systems for contactless on-chip cell and particle manipulation where it is possible to move and spatially localize these particles based on the different acoustophysical properties. While it was initially suggested that the acoustic forces could be harmful to the cells and could impact cell viability, proliferation, or function via phenotypic or even genotypic changes, further studies disproved such claims. This review is summarizing some interesting applications of acoustofluidics in the manipulations of biomaterials, such as cells or subcellular vesicles, in works published mainly within the last 5 years.

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“…Microfluidic-based nano-/submicroparticle enrichment methods have rapidly progressed and attracted great attentions due to the advantages of less sample need, easy operation, and high efficiency. Based on the external force fields, acoustofluidic [4,[12][13][14][15][16][17], electrophoresis [18,19], dielectrophoresis [20], magnetophoresis [21], thermophoresis [22][23][24], and optical tweezers [25] have been utilized for the enrichment of the nano-/submicroparticle with high efficiency and precision. For instance, driven by the acoustic streaming induced drag force and the elastic lift force, 800 nm particles can be efficiently concentrated and patterned near a micropillar using a an ultralow frequency [16].…”

Section: Introductionmentioning

confidence: 99%

Efficient microfluidic enrichment of nano‐/submicroparticle in viscoelastic fluid

et al. 2021

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The enrichment and focusing of the nano‐/submicroparticle (e.g., 150–1000 nm microvesicle shed from the plasma membrane) in the viscoelastic fluid has great potentials in the biomedical and clinical applications such as the disease diagnosis and the prognostic test for liquid biopsy. However, due to the small size and the resulting weak hydrodynamic force, the efficient manipulation of the nano‐/submicroparticle by the passive viscoelastic microfluidic technology remains a major challenge. For instance, a typically long channel length is often required to achieve the focusing or the separation of the nano‐/submicroparticle, which makes it difficult to be integrated in small chip area. In this work, a microchannel with gradually contracted cross‐section and high aspect ratio (the ratio of the height to the average width of channel) is utilized to enhance the hydrodynamic force and change the force direction, eventually leading to the efficient enrichment of nano‐/submicroparticles (500 and 860 nm) in a short channel length (2 cm). The influence of the flow rate, the particle size, the solid concentration, and the channel geometry on the enrichment of the nano‐/submicroparticles are investigated. With simple structure, small footprint, easy operation, and good performance, the present device would be a promising platform for various lab‐chip microvesicle‐related biomedical research and disease diagnosis.

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Exosome trapping and enrichment using a sound wave activated nano-sieve (SWANS)

Abstract: Exosomes, a form of extracellular vesicle, are an important precursor in regenerative medicine. Microfluidic methods exist to capture these sub-micrometer sized objects from small quantities of sample, ideal for multiple...

Cited by 37 publications

References 51 publications

Multinodal Acoustic Trapping Enables High Capacity and High Throughput Enrichment of Extracellular Vesicles and Microparticles in miRNA and MS Proteomics Studies

Multinodal Acoustic Trapping Enables High Capacity and High Throughput Enrichment of Extracellular Vesicles and Microparticles in miRNA and MS Proteomics Studies

Acoustofluidic platforms for particle manipulation

Efficient microfluidic enrichment of nano‐/submicroparticle in viscoelastic fluid

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