Exosome secretion and red cell maturation: Exploring molecular components involved in the docking and fusion of multivesicular bodies in K562 cells

Fader, Claudio Marcelo; Savina, Ariel; Sánchez, Diego; Colombo, María Isabel

doi:10.1016/j.bcmd.2005.07.002

Cited by 36 publications

(32 citation statements)

References 41 publications

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“…Rab5's role in autophagic vesicle fusion to the early endosome compartment, if any, is not particularly clear. On the other hand, Rab11's role in the autophagic process during erythrocyte maturation has been well documented by Fader et al [45,71,72], using the erythroleukemia cell line K562 as a model. In these cells, Rab11 is found to label MVB membranes.…”

Section: Endocytic Rabs and The Maturation Of Autophagic Vesiclesmentioning

confidence: 99%

Involvement of members of the Rab family and related small GTPases in autophagosome formation and maturation

Chua

Gan

Tang

2011

Cell. Mol. Life Sci.

113

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Macroautophagy, the process by which cytosolic components and organelles are engulfed and degraded by a double-membrane structure, could be viewed as a specialized, multistep membrane transport process. As such, it intersects with the exocytic and endocytic membrane trafficking pathways. A number of Rab GTPases which regulate secretory and endocytic membrane traffic have been shown to play either critical or accessory roles in autophagy. The biogenesis of the pre-autophagosomal isolation membrane (or phagophore) is dependent on the functionality of Rab1. A non-canonical, Atg5/Atg7-independent mode of autophagosome generation from the trans-Golgi or endosome requires Rab9. Other Rabs, such as Rab5, Rab24, Rab33, and Rab7 have all been shown to be required, or involved at various stages of autophagosomal genesis and maturation. Another small GTPase, RalB, was very recently demonstrated to induce isolation membrane formation and maturation via its engagement of the exocyst complex, a known Rab effector. We summarize here what is now known about the involvement of Rabs in autophagy, and discuss plausible mechanisms with future perspectives.

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Section: Endocytic Rabs and The Maturation Of Autophagic Vesiclesmentioning

confidence: 99%

Involvement of members of the Rab family and related small GTPases in autophagosome formation and maturation

Chua

Gan

Tang

2011

Cell. Mol. Life Sci.

113

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“…Exosome secretion was first described in hematopoïetic cell types, such as reticulocytes [9,10], B and T lymphocytes [11,12], platelets [13], mast cells [14], and dendritic cells [4]. Other cell types also produce exosomes: tumor cells [15], intestinal epithelial cells [16], astrocytes and neurons [17].…”

Section: Exosome Characterization Exosome Genesismentioning

confidence: 99%

“…Therefore, the "intraluminal vesicles" become "exosomes". The pioneering studies in reticulocytes (which lack lysosomes) demonstrated that MVBs and their contents, by fusing with the cell surface, eliminate obsolete proteins that do not follow an intracellular degradation pathway [9,10].…”

Section: Exosome Characterization Exosome Genesismentioning

confidence: 99%

Prospects for exosomes in immunotherapy of cancer

Mignot

Roux

Théry

et al. 2006

Journal of Cellular and Molecular Medicine

163

130

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Exosomes are nanometer sized membrane vesicles invaginating from multivesicular bodies and secreted from epithelial and hematopoietic cells. They were first described “ in vitro ” but vesicles with the hallmarks of exosomes are present in vivo in germinal centers and biological fluids. Their protein and lipid composition are unique and could account for their expanding functions such as eradication of obsolete proteins, antigen presentation or “Trojan horses” for viruses or prions. Exosome secretion could be a regulated process participating in the transfer of molecules inbetween immune cells. Despite numerous questions pertaining to their biological relevance, the potential of dendritic cell derived-exosomes as cell-free cancer vaccines is currently being assessed. This review will summarize the composition and formation of exosomes, preclinical data, Phase I trials and optimization protocols for improving their immunogenicity in tumor bearing patients.

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“…Exosome secretion was described to originate from haematopoietic cells such as reticulocytes [7][8][9], B lymphocytes and T cells [7,[10][11][12][13][14][15], platelets [7,10,[12][13][14][15][16], mast cells [7,10,[12][13][14][15]17], dendritic cells [7,10,[12][13][14] and macrophages [18,19]. Exosomes are also produced by the cells of non-haematopoietic origin like epithelial cells (intestinal epithelial cells) [7,12,[20][21][22][23][24][25][26][27], Schwann cells [28], astrocytes [7,29], neurons [7,29], melanocytes [12], mesothelioma cells [12], adipocytes …”

Section: Introductionmentioning

confidence: 99%

Exosomes – Structure, Biogenesis and Biological Role in Non‐Small‐Cell Lung Cancer

et al. 2014

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Many different cells produce and release membraneous microvesicles (MV) or exosomes into their microenvironment. Exosomes represent a specific subtype of secreted derived vesicles which are defined as homogenous vesicles of 30-100 nm lined by a lipid bilayer, which contain a specific set of proteins, lipids, and nucleic acids. There are clear evidences that they serve as important biological signals messengers and carriers in physiological as well as in pathological processes. Those derived from tumours (tumour-derived exosomes, TDexosomes) function as protumourigenic factors that can mediate intercellular communication in the tumour microenvironment and also contribute to cancer progression. The main functions of exosomes in the cancer microenvironment include the following: promotion of primary cancer growth, stimulation of angiogenesis, activation of stromal fibroblasts, sculpting the cancer ECM, generation of a premetastatic niche and suppression of host immune response. Exosomes have recently emerged as potentially promising diagnostic and prognostic biomarkers in cancer and other diseases. This article is a summary of information about the structure and origin of exosomes and also indicates the importance of exosomes and microRNAs in lung cancer. The role of exosomes in NSCLC is little known, and its explanation requires thorough research.

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Exosome secretion and red cell maturation: Exploring molecular components involved in the docking and fusion of multivesicular bodies in K562 cells

Cited by 36 publications

References 41 publications

Involvement of members of the Rab family and related small GTPases in autophagosome formation and maturation

Involvement of members of the Rab family and related small GTPases in autophagosome formation and maturation

Prospects for exosomes in immunotherapy of cancer

Exosomes – Structure, Biogenesis and Biological Role in Non‐Small‐Cell Lung Cancer

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