Exosome‐mediated transfer of miR‐1260b promotes cell invasion through Wnt/β–catenin signaling pathway in lung adenocarcinoma

Xia, Yang; Wei, Ke; Hu, Liu‐Qing; Zhou, Cun‐Rong; Lu, Zhi‐Bin; Zhan, Gen‐Sheng; Pan, Xinliang; Pan, Chunfeng; Wang, Jun; Wen, Wei; Xu, Jing; He, Zhicheng; Huang, Chenjun; Liang, Changhong

doi:10.1002/jcp.29578

Cited by 46 publications

(30 citation statements)

References 37 publications

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“…In a previous study, miR-301a was found to induce EMT in prostate cancer cells through inhibition of p63, which caused the release of ZEB1/2 in a miRNA-mediated inhibition and induction EMT [40]. Exosomal miR-1260b reportedly activates the Wnt/β-catenin signaling pathway in lung adenocarcinoma cells [41]. miR-1260b was suggested to directly suppress suppressor of cytokine singling 6 (SOCS6) expression and activate KIT proto-oncogene (KIT) signaling in non-small-cell lung cancer (NSCLC) [42].…”

Section: β-Catenin Signaling Pathwaymentioning

confidence: 89%

The Emerging Roles of Exosomes as EMT Regulators in Cancer

Kim

Lee

Shin

et al. 2020

Cells

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Epithelial–mesenchymal transition (EMT) causes epithelial cells to lose their polarity and adhesion property, and endows them with migratory and invasive properties to enable them to become mesenchymal stem cells. EMT occurs throughout embryonic development, during wound healing, and in various pathological processes, including tumor progression. Considerable research in the last few decades has revealed that EMT is invariably related to tumor aggressiveness and metastasis. Apart from the interactions between numerous intracellular signaling pathways known to regulate EMT, extracellular modulators in the tumor microenvironment also influence tumor cells to undergo EMT, with extracellular vesicles (EVs) receiving increasing attention as EMT inducers. EVs comprise exosomes and microvesicles that carry proteins, nucleic acids, lipids, and other small molecules to stimulate EMT in cells. Among EVs, exosomes have been investigated in many studies, and their role has been found to be significant with respect to regulating intercellular communications. In this review, we summarize recent studies on exosomes and their cargoes that induce cancer-associated EMT. Furthermore, we describe the possible applications of exosomes as promising therapeutic strategies.

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Section: β-Catenin Signaling Pathwaymentioning

confidence: 89%

The Emerging Roles of Exosomes as EMT Regulators in Cancer

Kim

Lee

Shin

et al. 2020

Cells

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“…Recently, several studies have reported the predictive potential of EV-miRNAs from plasma/serum for early detection in different cancers ( 19 – 21 ). For instance, EV-miR-1260b in plasma was upregulated in lung adenocarcinoma and promoted cancer cell invasion by regulating Wnt/β-catenin signaling pathway ( 22 ). Low EV-miR-548c-5p in serum was associated with poor prognosis in CRC ( 23 ).…”

Section: Introductionmentioning

confidence: 99%

Liquid Biopsy of Extracellular Vesicle-Derived miR-193a-5p in Colorectal Cancer and Discovery of Its Tumor-Suppressor Functions

et al. 2020

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Previously, abnormal extracellular vesicle (EV) sorting of miR-193a was identified in colorectal cancer (CRC) progression. Although a reduced level of miR-193a-5p in plasma/serum has been reported in many different types of cancer, the EV-derived miR-193a-5p level in CRC and its potential application as a minimally invasive biomarker are still unknown. Here, we evaluated the circulating EV-derived miR-193a-5p expression levels in a cohort of 101 participants by real-time quantitative polymerase chain reaction (RT-qPCR). We found that plasma EV-miR-193a-5p decreased significantly in CRC patients as compared with precancerous colorectal adenoma (CA) and non-cancerous control (NC) individuals. The circulating EV-miR-193a-5p showed an area under the receiver operating characteristic curve (AUC) of 0.740 in distinguishing CRC from CA and an AUC of 0.759 in distinguishing CRC from NC. Furthermore, the suppression on CRC cells of miR-193a-5p was verified by transwell, MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium), EdU, RT-qPCR, and western blotting. Bioinformatic analysis predicted 32 genes, which were the most likely miR-193a-5p targeted and mainly focused on tumor progression. Among them, we revealed that miR-193a-5p could inhibit CRC migration and invasion via targeting tumor-associated genes like CUT-like homeobox 1 (CUX1) and intersectin 1 (ITSN1). In conclusion, miR-193a-5p could suppress CRC development, and decreased plasma EV-miR-193a-5p could be a promising biomarker for human CRC detection.

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“…Xia et al [ 75 ] have demonstrated the uptake of EVs and the delivery of functional miRNAs in different cell lines. The exosomal-miR-1260b was found to be crucial for the activation of the Wnt/β-catenin signalling and the invasivness of lung adenocarcinoma cells.…”

Section: Extracellular Vesicles and The Wnt Pathwaymentioning

confidence: 99%

The Wnt Signalling Pathway: A Tailored Target in Cancer

Koni

Pinnarò

Brizzi

2020

IJMS

114

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Cancer is one of the greatest public health challenges. According to the World Health Organization (WHO), 9.6 million cancer deaths have been reported in 2018. The most common cancers include lung, breast, colorectal, prostate, skin (non-melanoma) and stomach cancer. The unbalance of physiological signalling pathways due to the acquisition of mutations in tumour cells is considered the most common cancer driver. The Wingless-related integration site (Wnt)/β-catenin pathway is crucial for tissue development and homeostasis in all animal species and its dysregulation is one of the most relevant events linked to cancer development and dissemination. The canonical and the non-canonical Wnt/β-catenin pathways are known to control both physiological and pathological processes, including cancer. Herein, the impact of the Wnt/β-catenin cascade in driving cancers from different origin has been examined. Finally, based on the impact of Extracellular Vesicles (EVs) on tumour growth, invasion and chemoresistance, and their role as tumour diagnostic and prognostic tools, an overview of the current knowledge linking EVs to the Wnt/β-catenin pathway is also discussed.

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Exosome‐mediated transfer of miR‐1260b promotes cell invasion through Wnt/β–catenin signaling pathway in lung adenocarcinoma

Cited by 46 publications

References 37 publications

The Emerging Roles of Exosomes as EMT Regulators in Cancer

The Emerging Roles of Exosomes as EMT Regulators in Cancer

Liquid Biopsy of Extracellular Vesicle-Derived miR-193a-5p in Colorectal Cancer and Discovery of Its Tumor-Suppressor Functions

The Wnt Signalling Pathway: A Tailored Target in Cancer

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