Exosome-mediated transfer of miR-10b promotes cell invasion in breast cancer

Singh, Ramesh; Pochampally, Radhika; Watabe, Kounosuke; Lü, Zhaohui; Mo, Yin‐Yuan

doi:10.1186/1476-4598-13-256

Cited by 341 publications

(264 citation statements)

References 38 publications

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“…miR-10b was identified as an important component promoting invasion of breast cancer cells (40). Transfer of MDA-MB 231 overexpressed miR-10b in exosomes to immortalized mammary epithelial cells (HMLE) induced invasion in this cell line.…”

Section: Exosomes Derived From Cancer Cells As Mediators Of Migrationmentioning

confidence: 99%

The Multiple Roles of Exosomes in Metastasis

Weidle

Birzele

Kollmorgen

et al. 2017

CGP

167

133

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Abstract. Exosomes are important contributors to cellMetastases are responsible for the death of a large majority of cancer patients despite considerable progress in surgical techniques, radiotherapy, chemotherapy and targeted therapies including immuno-therapy (1). A dramatic reduction of metastatic burden has been observed, however, tumor elimination is almost always incomplete. This phenomenon is based on drug resistance, which is due to adaptation of intracellular pathways or on activation of survival-supporting autocrine and paracrine pathways and several secreted factors expressed by drug-sensitive tumor cells after therapy (2). Metastasis can occur through release of cancer cells from the primary tumor into body cavities that holds true for ovarian and CNS tumors, or via hematogeneous and lymphatic vessels of the circulatory system (3). Metastases of some tumors are directed besides to lymph nodes to mainly one type of organ only, such as prostate cancer to the bones, pancreatic cancer and uveal melanoma to the liver, whereas tumors such as melanoma, breast-and lung cancer can colonize several types of organs (3). Tumor cells have been found in the blood of patients with early-stage cancer and in some cases even before the primary tumor has been diagnosed (4, 5). Recently, making use of single-cell expression profiling, it has been shown that early metastatic cells possess a stem-like gene signature and give rise to heterogeneous metastases (6). The metastatic process is characterized by a defined sequence of events (7,8). Initial steps are detachment from the extracellular matrix (ECM), invasion into the surrounding tissue and proteolysis of the basement membrane. After intravasation, survival of circulating tumor cells (CTCs) is achieved by forming clusters, binding to platelets and immune evasion. Subsequently, they arrest in distal microvascular beds and extravasation can be achieved either by migration through intercellular junctions of endothelial cells (EC) or penetration of a single EC (9). CTCs can also colonize their tumors of origin, a process referred to as "tumor self-seeding", selecting for cancer cell populations more aggressive than those present in the primary tumor (10). After extravasation, colonization and outgrowth in the parenchyma of distant organs are the following steps. A common theme of the metastatic process is settlement of disseminated tumor cells (DTCs) into latency (dormancy), which can last from several months to decades (11). It has been observed that DTCs are recruited into pre-metastatic niches that support their survival by interactions with endothelial, myeloid cells, fibroblasts and other types of cells. After adaptation to the host microenvironment and suppression of an anti-tumoral immune response, DTCs are activated by not yet completely resolved mechanisms. Finally their outgrowth is based on an angiogenic switch mediated by pro-angiogenic factors and establishment of a vascular network to support the metabolic demands of colonizing tumor cells (12). In the follo...

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Section: Exosomes Derived From Cancer Cells As Mediators Of Migrationmentioning

confidence: 99%

The Multiple Roles of Exosomes in Metastasis

Weidle

Birzele

Kollmorgen

et al. 2017

CGP

167

133

View full text Add to dashboard Cite

show abstract

“…Additionally, miR-10b was highly secreted into medium via exosomes. nSMase2 promotes the loading of miR-10b into exosomes of breast cancer cells [63]. Upon co-culturing of those tumoral miR-10b loaded exosomes with non-metastatic mammary epithelial breast cells (HMLE cells), the HMLE cells acquired invasion ability [63].…”

Section: Exosomic Mirs As Key Drivers Of Intercellular Communicationmentioning

confidence: 99%

Exosomic microRNAs as emerging key regulators of intercellular communication in the tumor microenvironment and beyond

Murtadha¹,

Fabbri²

2016

microRNA Diagnostics and Therapeutics

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MicroRNAs (miRs) are small non-coding RNAs with key gene regulatory functions. Recent evidence has shown that miRs have a central role in shaping the biology of the Tumor Microenvironment (TME). The discovery that some exosomes contain high levels of miR cargo that shuttle between cells and mediate intercellular cross-talk has shifted the focus of miR research towards understanding the biological role of exosomic miRs. In this review, we highlight the emerging role of exosomic miRs in molding the tumor microenvironment towards pro-tumor conditions by altering intercellular communication. We briefly discuss some mechanisms of selective loading of miRs into exosomes, as well as emerging evidence that exosomic miRs are present in all biological fluids. Furthermore, we describe the differences in the exosomic miR signatures between cancer patients and healthy controls, and the potential role of exosomic miRs as diagnostic, prognostic, and therapeutic biomarkers.

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“…Exosomes were prepared according to the methods published previously (Singh et al, 2014). ADSC (or MCF-7) culture medium (48 h) was collected and centrifuged at 500 × g for 10 min and then recentrifuged at 2000 × g for 20 min to remove cells.…”

Section: Exosome Extraction and Quantificationmentioning

confidence: 99%

Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro:a study emphasizing signaling molecule expression and transcriptional changes

Wu¹,

Jin²,

Chen³

et al. 2017

Turk J Biol

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IntroductionAdipose-derived mesenchymal stem cells (ADSCs) are a heterogeneous population of adult stem cells derived from adipose tissue. ADSCs functionally differentiate into various cell lineages in a special microenvironment (Uzbas et al., 2015). ADSCs are autologous, available in sufficient quantities in most patients, and are widely used in soft tissue repair, for instance, in breast reconstruction in surgical patients (Rowan et al., 2014). However, recent papers indicate ADSCs potentially induce a tumor microenvironment and affect tumor cell biology. In mammary tissue (Muehlberg et al., 2009), resident stem cells promote breast cancer metastasis, home to the tumor site, and stimulate tumor growth not only when coinjected locally but also when injected intravenously. Kucerova et al. (2011) reported that ADSCs could stimulate proliferation of several breast cancer cells in vitro, such as MDA-MB-361, T47D, and EGFP-MCF-7. Kim et al. (2013) found that breast and abdominal adipose tissues have comparable gene expression profiles and are comparable in supporting breast cancer cell proliferation and metastasis. On the other hand, it has been previously reported that the morphology and phenotype of ADSCs are altered when cocultured with tumor cells. ADSCs (Jotzu et al., 2011) that received tumor-derived factors differentiated into carcinoma-associated fibroblast-like cells, which functionally contribute to tumor growth, invasion, and metastasis. ADSCs directly cocultured with H358 lung cancer cells differentiated into tumor stroma that play a supportive role during cancer progression (Park et al., 2013).Of note, except for the diffusible factors (cytokines, chemokine, and growth factors), cell-derived exosomes have been described as a new signal in cell-to-cell communication. Exosomes are membrane vesicles (30-100 nm) from luminal membranes of multivesicular bodies and are constitutively released upon fusion with cell membranes (Tan et al., 2016). It has been previously confirmed that ADSCs secrete exosomes (Cho et al., 2012;Lin et al., 2013), promote MCF-7 cell migration, and that exosomes from breast cancer cells convert ADSCs into myofibroblast-like cells. These results provide evidence that exosomes play a role in crosstalk between ADSCs and MCF-7 cells.

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Exosome-mediated transfer of miR-10b promotes cell invasion in breast cancer

Cited by 341 publications

References 38 publications

The Multiple Roles of Exosomes in Metastasis

The Multiple Roles of Exosomes in Metastasis

Exosomic microRNAs as emerging key regulators of intercellular communication in the tumor microenvironment and beyond

Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro:a study emphasizing signaling molecule expression and transcriptional changes

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