Exosome-mediated remodeling of the tumor microenvironment: From local to distant intercellular communication

Huang, Yujuan; Kanada, Masamitsu; Ye, Jiaxiang; Deng, Yayan; He, Qian; Lei, Zhengyang; Chen, Yong; Li, Yongqiang; Qin, Peiwu; Zhang, Jinyan; Wei, Jiazhang

doi:10.1016/j.canlet.2022.215796

Cited by 40 publications

(25 citation statements)

References 147 publications

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“…Besides, there are also clinical trials centered on EV as therapeutic tools or delivery drugs. With in-depth understanding of EVs as well as technological progress, EV-based drugs may become a potential therapeutic intervention target for treatment of diseases ( 26 ).…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles derived from fibroblasts induced with or without high glucose exert opposite effects on wound healing and angiogenesis

Bian

Tang

et al. 2022

Front. Surg.

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BackgroundCommunication between fibroblasts and endothelial cells is essential for skin wound repair and regeneration. Extracellular vesicles (EVs) are crucial for intracellular communication by transporting active molecules. However, whether EVs derived from diabetic fibroblasts can perform the nomal communication function is unclear. Here, we compared the effects of EVs from human skin fibroblasts (HSFs) induced with or without HG on the angiogenic function of endothelial cells and wound healing.MethodsWe first collected EVs from HSFs cultured with normal glucose concentration (NG-EVs) or with HG concentration (HG-EVs) and applied them to treat human umbilical vein endothelial cells (HUVECs). The cells were divided into three groups: control group, NG-EVs group, and HG-EVs group. We then examined the proliferation, migration, apoptosis, and tube formation of HUVECs. To illustrate the mechanism, the expression of β-catenin, GSK-3β, and p-GSK-3β was detected by western-blot. Finally, NG-EVs or HG-EVs were used to treat the wounds of mice to determine their role in wound closure.ResultsBy DNA content detection, Annexin V/PI staining, and EdU staining, we found that NG-EVs promoted HUVEC proliferation, while HG-EVs exhibited an opposite effect (p < 0.05). Scratch assay and tube formation assay demonstrated that NG-EV promoted angiogenesis in vitro, while HG-EVs showed negative impact (p < 0.05). The expressions of β-catenin and p-GSK-3β in HUVECs were enhanced by NG-EVs and decreased by HG-EVs (p < 0.05). Additionally, the in vivo experiment demonstrated that NG-EVs effectively promoted wound healing by locally enhancing blood supply and angiogenesis. In contrast, HG-EVs leaded to delayed wound closure and reduced blood supply and angiogenesis (p < 0.05).ConclusionNG-EVs and HG-EVs exert opposite effects on wound healing and angiogenesis possibly by regulating GSK-3β/β-catenin signaling pathway. This research may provide a new treatment strategy for wound healing and illustrate the mechanism for impaired angiogenesis in diabetics.

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Section: Discussionmentioning

confidence: 99%

Extracellular vesicles derived from fibroblasts induced with or without high glucose exert opposite effects on wound healing and angiogenesis

Bian

Tang

et al. 2022

Front. Surg.

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“…Thus, targeting exosomes or inhibiting the ability of cells to receive signals from exosomes could interrupt cancer progression and metastasis. Increasing number of studies are focusing on the tumor-derived exo-miRNA mediated intercellular cross talk and remodeling of the tumor microenvironment ( 43 ). Comprehensive understanding of the underlying mechanisms is necessitated to combat this deadly disease.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Recent advances in the roles of exosomal microRNAs in neuroblastoma

Bhavsar

2023

Front. Oncol.

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Exosomal miRNAs (exo-miRs), universally found in biofluids, tissues, and/or conditioned medium of the cell cultures play a significant role in cell - cell communication, thus driving cancer progression and metastasis. Very few studies have explored the role of exo-miRs in the progression of children’s cancer - neuroblastoma. In this mini review, I briefly summarize the existing literature on the role of exo-miRs in the pathogenesis of neuroblastoma.

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“…In recent years, accumulating evidence has suggested that sEVs play important roles in chronic hepatitis, cirrhosis, and liver cancer [9][10][11]. sEVs from HCC transport LOXL4 and activate the FAK/Src pathway to facilitate the migration of HCC cells [12].…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma

et al. 2022

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Purpose Liver cancer is one of the most common tumors with the seventh-highest incidence and the third-highest mortality. Many studies have shown that small extracellular vesicles (sEVs) play an important role in liver cancer. Here, we report comprehensive signatures for sEV proteins from plasma obtained from patients with hepatocellular carcinoma (HCC), which might be valuable for the evaluation and diagnosis of HCC. Methods We extracted sEVs from the plasma of controls and patients with HCC. Differentially expressed proteins in the sEVs were analyzed using label-free quantification and bioinformatic analyses. Western blotting (WB) was used to validate the abovementioned sEV proteins. Results Proteomic analysis was performed for plasma sEVs from 21 patients with HCC and 15 controls. Among the 335 identified proteins in our study, 27 were significantly dysregulated, including 13 upregulated proteins that were involved predominantly in the complement cascade (complement C1Q subcomponent subunit B (C1QB), complement C1Q subcomponent subunit C (C1QC), C4B-binding protein alpha chain (C4BPA), and C4B-binding protein beta chain (C4BPB)) and the coagulation cascade (F13B, fibrinogen alpha chain (FGA), fibrinogen beta chain (FGB), and fibrinogen gamma chain (FGG)). We verified increased levels of the C1QB, C1QC, C4BPA, and C4BPB proteins in the plasma sEVs from patients with HCC in both the discovery cohort and validation cohort. Conclusions The complement cascade in sEVs was significantly involved in HCC progression. C1QB, C1QC, C4BPA, and C4BPB were highly abundant in the plasma sEVs from patients with HCC and might represent molecular signatures.

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Exosome-mediated remodeling of the tumor microenvironment: From local to distant intercellular communication

Cited by 40 publications

References 147 publications

Extracellular vesicles derived from fibroblasts induced with or without high glucose exert opposite effects on wound healing and angiogenesis

Extracellular vesicles derived from fibroblasts induced with or without high glucose exert opposite effects on wound healing and angiogenesis

Recent advances in the roles of exosomal microRNAs in neuroblastoma

Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma

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