Exosome-mediated intracellular signalling impacts the development of endometriosis—new avenues for endometriosis research

Schjenken, John E.; Panir, Kavita; Robertson, Sarah A.; Hull, Louise

doi:10.1093/molehr/gay050

Cited by 16 publications

(9 citation statements)

References 21 publications

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“…Exosomes are of increasing interest in the pathophysiology of endometriosis, and a recent review by Schjenken et al (68) asked that ''future studies should examine the composition of these exosomes,'' referring to exosomes produced by endometriotic lesions and released into the PF. We believe to have made the first step in that direction.…”

Section: Discussionmentioning

confidence: 99%

Characterization of exosomes in peritoneal fluid of endometriosis patients

Nazri

Imran

Fischer

et al. 2020

Fertility and Sterility

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Objective: To demonstrate the feasibility of studying exosomes directly from peritoneal fluid, we isolated exosomes from endometriosis patient samples and from controls, and characterized their cargo. Design: Case-control experimental study. Setting: Academic clinical center. Patient (s): Women with and without endometriosis who underwent laparoscopic surgery (n ¼ 28 in total). Intervention (s): None. Main Outcome Measure (s): Concentration of exosomes within peritoneal fluid and protein content of the isolated exosomes. Result (s): Peritoneal fluid samples were pooled according to the cycle phase and disease stage to form six experimental groups, from which the exosomes were isolated. Exosomes were successfully isolated from peritoneal fluid in all the study groups. The concentration varied with cycle phase and disease stage. Proteomic analysis showed specific proteins in the exosomes derived from endometriosis patients that were absent in the controls. Five proteins were found exclusively in the endometriosis groups: PRDX1, H2A type 2-C, ANXA2, ITIH4, and the tubulin a-chain. Conclusion (s):Exosomes are present in peritoneal fluid. The characterization of endometriosis-specific exosomes opens up new avenues for the diagnosis and investigation of endometriosis.

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Section: Discussionmentioning

confidence: 99%

Characterization of exosomes in peritoneal fluid of endometriosis patients

Nazri

Imran

Fischer

et al. 2020

Fertility and Sterility

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“…EVs have been isolated and characterized from peritoneal fluid, serum, cultured stromal cells from eutopic and ectopic endometrium, and peritoneal macrophages of women with endometriosis 70,93‐96 . Altered expression of genes associated with EVs is also observed in endometriotic tissue as compared to eutopic endometrium 36,97,98 …”

Section: Extracellular Vesicles In Disorders Of Endometriummentioning

confidence: 99%

“…70,[93][94][95][96] Altered expression of genes associated with EVs is also observed in endometriotic tissue as compared to eutopic endometrium. 36,97,98 Endometriosis-associated EVs carry unique cargo including transcription factors, cell-surface receptors, cytosolic and nuclear proteins, miRNA and mRNA that contribute to disease pathophysiology by regulating inflammation, angiogenesis and proliferation within the endometriotic tissues microenvironment. EVs are found to be associated with the abnormal immunological microenvironment by regulating immune cell infiltration and the production of cytokines.…”

Section: Extracellular Vesicles In Endometriosismentioning

confidence: 99%

Extracellular vesicles in embryo implantation and disorders of the endometrium

Mishra

Ashary

Sharma

et al. 2020

American J Rep Immunol

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Implantation of the embryo is a rate‐limiting step for a successful pregnancy, and it requires an intricate crosstalk between the embryo and the endometrium. Extracellular vesicles (EVs) are membrane‐enclosed, nano‐sized structures produced by cells to mediate cell to cell communication and modulate a diverse set of biological processes. Herein, we review the involvement of EVs in the process of embryo implantation and endometrial diseases. EVs have been isolated from uterine fluid, cultured endometrial epithelial/stromal cells and trophectodermal cells. The endometrial epithelial and stromal/decidual cell‐derived EVs and its cargo are internalized bythe trophoblast cells, and they regulate a diverse set of genes involved in adhesion, invasion and migration. Conversely, the embryo‐derived EVs and its cargo are internalized by epithelial and immune cells of the endometrium for biosensing and immunomodulation required for successful implantation. EVs have also been shown to play a role in infertility, recurrent implantation failure, endometriosis, endometritis and endometrial cancer. Further research should set a stage for EVs as non‐invasive “liquid biopsy” tools for assessment of endometrial health.

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“…These findings from cancer and endometrium studies raise the possibility that macrophage-derived and endometriosis tissue-specific sEVs could have an impact on endometriosis progression through concerted cell targeting in the lesion microenvironment ( 76 – 78 ). Indeed, similar to TAM, pM Φ are also regulated by sEV mediated signalling networks.…”

Section: Small Extracellular Vesicle-mediated Crosstalk In Tissue-spe...mentioning

confidence: 92%

Macrophages and small extracellular vesicle mediated-intracellular communication in the peritoneal microenvironment: Impact on endometriosis development

Wang

Dragovic

Greaves

et al. 2023

Front. Reprod. Health

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Endometriosis is an inflammatory disease that is defined as the growth of endometrium-like tissue outside the uterus, commonly on the lining of the pelvic cavity, visceral organs and in the ovaries. It affects around 190 million women of reproductive age worldwide and is associated with chronic pelvic pain and infertility, which greatly impairs health-related life quality. The symptoms of the disease are variable, this combined with a lack of diagnostic biomarkers and necessity of surgical visualisation to confirm disease, the prognosis can take an average timespan of 6–8 years. Accurate non-invasive diagnostic tests and the identification of effective therapeutic targets are essential for disease management. To achieve this, one of the priorities is to define the underlying pathophysiological mechanisms that contribute to endometriosis. Recently, immune dysregulation in the peritoneal cavity has been linked to endometriosis progression. Macrophages account for over 50% of immune cells in the peritoneal fluid and are critical for lesion growth, angiogenesis, innervation and immune regulation. Apart from the secretion of soluble factors like cytokines and chemokines, macrophages can communicate with other cells and prime disease microenvironments, such as the tumour microenvironment, via the secretion of small extracellular vesicles (sEVs). The sEV-mediated intracellular communication pathways between macrophages and other cells within the peritoneal microenvironment in endometriosis remain unclear. Here, we give an overview of peritoneal macrophage (pMΦ) phenotypes in endometriosis and discuss the role of sEVs in the intracellular communication within disease microenvironments and the impact they may have on endometriosis progression.

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Exosome-mediated intracellular signalling impacts the development of endometriosis—new avenues for endometriosis research

Cited by 16 publications

References 21 publications

Characterization of exosomes in peritoneal fluid of endometriosis patients

Characterization of exosomes in peritoneal fluid of endometriosis patients

Extracellular vesicles in embryo implantation and disorders of the endometrium

Macrophages and small extracellular vesicle mediated-intracellular communication in the peritoneal microenvironment: Impact on endometriosis development

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