Exosome-enriched plasma analysis as a tool for the early detection of hypertensive gestations

Weingrill, Rodrigo Barbano; Paladino, Sandra Luft; Souza, Matheus Leite Ramos de; Pereira, Eduardo Manoel; Marques, Aldilane Lays Xavier; Fonseca, Eduardo J.S.; Ursolino, Jeferson Santana; Aquino, Thiago Mendonça de; Bevilacqua, Estela Maris Andrade Forell; Silva, Jean Carl; Borbely, Alexandre Urban

doi:10.1016/j.placenta.2021.07.058

Cited by 3 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence and abundance of these molecules in exosomes and EVs vary among cell types under different conditions (Chirackal et al., 2019; Jeppesen et al., 2019; Lian et al., 2017; Singhto & Thongboonkerd, 2018; Singhto et al., 2018). In other words, exosomes and EVs isolated from healthy and unhealthy individuals may contain differential levels of proteins (Kerr et al., 2018; Tian et al., 2021), DNAs (Hagey et al., 2021; Zhao et al., 2021), RNAs (Tan et al., 2021; Zhang et al., 2022), and metabolites (Tao et al., 2019; Weingrill et al., 2021; Zhu et al., 2021). Among the body fluid EVs, urinary exosomes and other small EVs ( altogether termed as “nanoscale uEVs” ) have gained a wide attention from researchers due to their availability and non‐invasiveness of the sample collection (Vitorino et al., 2021).…”

Section: Introductionmentioning

confidence: 99%

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Noonin

Peerapen

Thongboonkerd

2022

J of Extracellular Bio

View full text Add to dashboard Cite

Bacterial overgrowth is common for improperly stored urine. However, its effects on human urinary extracellular vesicles (uEVs) study had not been previously examined nor documented. This study investigated the presence of bacterial EVs (bEVs) contaminated in uEVs samples and their effects on uEVs study. Nanoscale uEVs were isolated from normal human urine immediately after collection (0-h) or after 25 • Cstorage with/without preservative (10 mM NaN 3 ) for up to 24-h. Turbidity, bacterial count and total uEVs proteins abnormally increased in the 8-h and 24-h-stored urine without NaN 3 . NanoLC-ESI-LTQ-Orbitrap MS/MS identified 6-13 bacterial proteins in these contaminated uEVs samples. PCR also detected bacterial DNAs in these contaminated uEVs samples. Besides, uEVs derived from 8-h and 24-h urine without NaN 3 induced macrophage activation (CD11b and phagocytosis) and secretion of cytokines (IFN-α, IL-8, and TGF-β) from macrophages and renal cells (HEK-293, HK-2, and MDCK). All of these effects induced by bacterial contamination were partially/completely prevented by NaN 3 . Interestingly, macrophage activation and cytokine secretion were also induced by bEVs purified from Escherichia coli. This study clearly shows evidence of bEVs contamination and their effects on human uEVs study when the urine samples were inappropriately stored, whereas NaN 3 can partially/completely prevent such effects from the contaminated bEVs.

show abstract

Section: Introductionmentioning

confidence: 99%

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Noonin

Peerapen

Thongboonkerd

2022

J of Extracellular Bio

View full text Add to dashboard Cite

show abstract

“…EVs carry cargo in the form of genetic material (small RNA species, including miRNAs), proteins, and lipids, and can transfer their heterogeneous cargo to target cells to induce biological responses 40 . For this reason, EVs have been considered as therapeutic agents for several conditions including those where multiple molecules and/or pathways are missing or dysregulated, such as asthma, chronic lung disease, diabetes, systemic hypertension, and cancer 41–49 …”

Section: New Insights Into Molecular Mechanisms Of Pulmonary Hypoplas...mentioning

confidence: 99%

“…40 For this reason, EVs have been considered as therapeutic agents for several conditions including those where multiple molecules and/or pathways are missing or dysregulated, such as asthma, chronic lung disease, diabetes, systemic hypertension, and cancer. [41][42][43][44][45][46][47][48][49]…”

Section: New Insights Into Molecular Mechanisms Of Pulmonary Hypoplas...mentioning

confidence: 99%

Fetal lung regeneration using stem cell‐derived extracellular vesicles: A new frontier for pulmonary hypoplasia secondary to congenital diaphragmatic hernia

et al. 2022

View full text Add to dashboard Cite

The poor outcomes of babies with congenital diaphragmatic hernia (CDH) are directly related to pulmonary hypoplasia, a condition characterized by impaired lung development. Although the pathogenesis of pulmonary hypoplasia is not fully elucidated, there is now evidence that CDH patients have missing or dysregulated microRNAs (miRNAs) that regulate lung development. A prenatal therapy that supplements these missing/dysregulated miRNAs could be a strategy to rescue normal lung development. Extracellular vesicles (EVs), also known as exosomes when of small dimensions, are lipid‐bound nanoparticles that can transfer their heterogeneous cargo (proteins, lipids, small RNAs) to target cells to induce biological responses. Herein, we review all studies that show evidence for stem cell‐derived EVs as a regenerative therapy to rescue normal development in CDH fetal lungs. Particularly, we report studies showing that administration of EVs derived from amniotic fluid stem cells (AFSC‐EVs) to models of pulmonary hypoplasia promotes fetal lung growth and maturation via transfer of miRNAs that are known to regulate lung developmental processes. We also describe that stem cell‐derived EVs exert effects on vascular remodeling, thus possibly preventing postnatal pulmonary hypertension. Finally, we discuss future perspectives and challenges to translate this promising stem cell EV‐based therapy to clinical practice.

show abstract

“…68 Furthermore, Weingrill et al found that plasma EVs could also be used to provide an initial assessment of placental function and to develop diagnosis, treatment, and prognosis of gestational hypertension. 88 Notably, for ST elevation myocardial infarction, evidence has demonstrated that plasma EVs in patients contain more lipid molecules, revealing possible biomarkers and signal transduction mechanisms. 69…”

mentioning

confidence: 99%

Metabolomics of Extracellular Vesicles: A Future Promise of Multiple Clinical Applications

Wu¹,

Chen²,

Guo³

et al. 2022

IJN

View full text Add to dashboard Cite

Extracellular vesicles (EVs) can contain DNA, RNA, proteins and metabolic molecules from primary origins; they are coated with a phospholipid bilayer membrane and released by cells into the extracellular matrix. EVs can be obtained from various body liquids, including the blood, saliva, cerebrospinal fluid, and urine. As has been proved, EVs-mediated transfer of biologically active molecules is crucial for various physiological and pathological processes. Extensive investigations have already begun to explore the diagnosis and prognosis potentials for EVs. Furthermore, research has continued to recognize the critical role of nucleic acids and proteins in EVs. However, our understanding of the comprehensive effects of metabolites in these nanoparticles is currently limited and in its infancy. Therefore, we have attempted to summarize the recent research into the metabolomics of EVs in relation to potential clinical applications and discuss the problems and challenges that have occurred, to provide more guidance for the future development in this field.

show abstract

Exosome-enriched plasma analysis as a tool for the early detection of hypertensive gestations

Cited by 3 publications

References 0 publications

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Fetal lung regeneration using stem cell‐derived extracellular vesicles: A new frontier for pulmonary hypoplasia secondary to congenital diaphragmatic hernia

Metabolomics of Extracellular Vesicles: A Future Promise of Multiple Clinical Applications

Contact Info

Product

Resources

About