Exosome derived from murine adipose-derived stromal cells: Neuroprotective effect on in vitro model of amyotrophic lateral sclerosis

Bonafede, Roberta; Scambi, Ilaria; Peroni, Daniele; Potrich, Valentina; Boschi, Federico; Benati, Donatella; Bonetti, Bruno; Mariotti, Raffaella

doi:10.1016/j.yexcr.2015.12.009

Cited by 154 publications

(140 citation statements)

References 35 publications

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“…To test the effects of exosomes in ALS, we have recently demonstrated that the administration of exosomes in an in vitro model of ALS exerts a neuroprotective effect following an oxidative insult, supporting the idea that exosomes can recapitulate and ameliorate the neuroprotective effect of stem cell therapy (Bonafede et al, 2016). …”

Section: Conclusion and Future Perspectivesmentioning

confidence: 53%

“…We recently demonstrated that exosomes isolated from ASC exert a neuroprotective effect in an in vitro model of ALS (Bonafede et al, 2016). In this study, we used the motoneuron-like cell line NSC-34 transiently and stable transfected with different SOD1 point mutations to mimic the behavior of ALS motoneurons, and H 2 O 2 as pathological insult.…”

Section: Extracellular Vesiclesmentioning

confidence: 99%

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ALS Pathogenesis and Therapeutic Approaches: The Role of Mesenchymal Stem Cells and Extracellular Vesicles

Bonafede

Mariotti

2017

Front. Cell. Neurosci.

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151

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Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive muscle paralysis determined by the degeneration of motoneurons in the motor cortex brainstem and spinal cord. The ALS pathogenetic mechanisms are still unclear, despite the wealth of studies demonstrating the involvement of several altered signaling pathways, such as mitochondrial dysfunction, glutamate excitotoxicity, oxidative stress and neuroinflammation. To date, the proposed therapeutic strategies are targeted to one or a few of these alterations, resulting in only a minimal effect on disease course and survival of ALS patients. The involvement of different mechanisms in ALS pathogenesis underlines the need for a therapeutic approach targeted to multiple aspects. Mesenchymal stem cells (MSC) can support motoneurons and surrounding cells, reduce inflammation, stimulate tissue regeneration and release growth factors. On this basis, MSC have been proposed as promising candidates to treat ALS. However, due to the drawbacks of cell therapy, the possible therapeutic use of extracellular vesicles (EVs) released by stem cells is raising increasing interest. The present review summarizes the main pathological mechanisms involved in ALS and the related therapeutic approaches proposed to date, focusing on MSC therapy and their preclinical and clinical applications. Moreover, the nature and characteristics of EVs and their role in recapitulating the effect of stem cells are discussed, elucidating how and why these vesicles could provide novel opportunities for ALS treatment.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 53%

Section: Extracellular Vesiclesmentioning

confidence: 99%

ALS Pathogenesis and Therapeutic Approaches: The Role of Mesenchymal Stem Cells and Extracellular Vesicles

Bonafede

Mariotti

2017

Front. Cell. Neurosci.

Self Cite

151

117

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“…Exosomes are naturally occurring biological nanovesicles utilized by cells to communicate signals to local or remote cells (Raposo and Stoorvogel, ). Based on accumulating evidence for the key role of exosomes in neuronal protection, nerve regeneration, remyelination, and synaptic plasticity (Kourembanas, ), an exosome‐based therapeutic approach for neurodegenerative diseases has been proposed (Bonafede et al., ; Maumus, Jorgensen, & Noel, ; Wei et al., ). The assessment of temporal and spatial homing of exosomes could provide further information on their site of action and could elucidate their mechanism of action.…”

Section: Commentarymentioning

confidence: 99%

Labeling and Magnetic Resonance Imaging of Exosomes Isolated from Adipose Stem Cells

et al. 2017

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Adipose stem cells (ASC) represent a promising therapeutic approach for neurodegenerative diseases. Most biological effects of ASC are probably mediated by extracellular vesicles, such as exosomes, which influence the surrounding cells. Current development of exosome therapies requires efficient and noninvasive methods to localize, monitor, and track the exosomes. Among imaging methods used for this purpose, magnetic resonance imaging (MRI) has advantages: high spatial resolution, rapid in vivo acquisition, and radiation-free operation. To be detectable with MRI, exosomes must be labeled with MR contrast agents, such as ultra-small superparamagnetic iron oxide nanoparticles (USPIO). Here, we set up an innovative approach for exosome labeling that preserves their morphology and physiological characteristics. We show that by labeling ASC with USPIO before extraction of nanovesicles, the isolated exosomes retain nanoparticles and can be visualized by MRI. The current work aims at validating this novel USPIO-based exosome labeling method by monitoring the efficiency of the labeling with MRI both in ASC and in exosomes. © 2017 by John Wiley & Sons, Inc.

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“…67 Another effect of NSCs on damaged neural cell populations is a paracrine effect via the release of exosomes, affecting cellcell communication. Bonafede et al 68 developed an in vitro model of ALS through motor neuron-like NSC-34 cells (NSCs that over express human ALS mutations SOD1 (G93A, G37R or A4V)) that exhibited oxidative stress found in ALS in vivo. The NSC-34 cells were protected from this stress, increasing cell viability, by treating the cells with exosomes derived from murine adipose-derived stromal cells.…”

Section: Neural Stem Cellsmentioning

confidence: 99%

Tissue engineered organoids for neural network modelling

Kose-Dunn¹,

Fricker²,

Roach³

2017

ATROA

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The increased prevalence of neurological diseases across the world has stimulated a great deal of research into the physiological and pathological brain, both at clinical and pre-clinical level. This has led to the development of many sophisticated tissue engineered neural models, presenting greater cellular complexity to better mimic the central nervous system niche environment. These have been developed with the ambition to improve pre-clinical assessment of pharma and cellular therapies, as well as better understand this tissue type and its function/dysfunction. This review covers the necessary considerations in in vitro model design, along with recent advances in 2Dculture systems, to 3D organoids and bio-artificial organs.

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Exosome derived from murine adipose-derived stromal cells: Neuroprotective effect on in vitro model of amyotrophic lateral sclerosis

Cited by 154 publications

References 35 publications

ALS Pathogenesis and Therapeutic Approaches: The Role of Mesenchymal Stem Cells and Extracellular Vesicles

ALS Pathogenesis and Therapeutic Approaches: The Role of Mesenchymal Stem Cells and Extracellular Vesicles

Labeling and Magnetic Resonance Imaging of Exosomes Isolated from Adipose Stem Cells

Tissue engineered organoids for neural network modelling

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