Exosomal vimentin from adipocyte progenitors accelerates wound healing

Parvanian, Sepideh; Yan, Fuxia; Su, Dandan; Coelho-Rato, Leila S.; Venu, Arun P.; Yang, Peiru; Zou, Xiaoheng; Jiu, Yaming; Chen, Hongbo; Eriksson, John E.; Cheng, Fang

doi:10.1002/cm.21634

Cited by 22 publications

(19 citation statements)

References 44 publications

(49 reference statements)

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“…The changes of osmotic pressure have adverse effects on water flux, cell volume, and signal pathways involved in cell proliferation, cell migration, and apoptosis. The change of exocrine body composition caused by stress is an effective adaptive mechanism, which can help cells regulate intracellular pressure conditions [ 24 , 25 ]. Osmotic pressure increases the size of exocrine bodies and enhances their release.…”

Section: Osmotic Pressurementioning

confidence: 99%

Review of the Role of Mesenchymal Stem Cells and Exosomes Derived from Mesenchymal Stem Cells in the Treatment of Orthopedic Disease

2022

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Exosomes can be derived from mesenchymal stem cells (MSCs) and have recently been used to treat defects in the tendons. They may also have applications in treating osteoporosis. MSC-derived exosomes communicate with mesenchymal and osteogenic cells through endocrine or paracrine mechanisms and contribute factors involved in physiological and pathological orthopedic conditions associated with hypoxia and bone tumors. Also, generalized medical conditions, such as obesity, hyperglycemia, and degenerative diseases, can inhibit the osteogenic effect of MSC-derived exosomes. This review aims to present an update on the roles of MSCs and exosomes derived from MSCs in treating orthopedic diseases, such as osteoporosis, and in the repair of cartilage, tendons, and bone fractures.

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Section: Osmotic Pressurementioning

confidence: 99%

Review of the Role of Mesenchymal Stem Cells and Exosomes Derived from Mesenchymal Stem Cells in the Treatment of Orthopedic Disease

2022

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“…For example, astrocytes devoid of vimentin showed a less effective response to osmotic stress [ 29 ]. Our laboratory and others have validated the incorporation of vimentin into the exosomes from different cell lines [ 30 , 31 , 32 , 33 , 34 ]. Exosomal vimentin can attach to the cell surface at distinct sites via specific cell-surface receptors and initiate a cellular response [ 30 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

“…Our laboratory and others have validated the incorporation of vimentin into the exosomes from different cell lines [ 30 , 31 , 32 , 33 , 34 ]. Exosomal vimentin can attach to the cell surface at distinct sites via specific cell-surface receptors and initiate a cellular response [ 30 , 35 ]. Here, prompted by our previous findings underlying the crucial role of exosomal vimentin from adipocyte progenitors (APCs) in mediating wound healing, we aimed to investigate its contribution to osmotic mechanical stress during wound healing.…”

Section: Introductionmentioning

confidence: 99%

Exosomal Vimentin from Adipocyte Progenitors Protects Fibroblasts against Osmotic Stress and Inhibits Apoptosis to Enhance Wound Healing

Parvanian

Zha

et al. 2021

IJMS

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Mechanical stress following injury regulates the quality and speed of wound healing. Improper mechanotransduction can lead to impaired wound healing and scar formation. Vimentin intermediate filaments control fibroblasts’ response to mechanical stress and lack of vimentin makes cells significantly vulnerable to environmental stress. We previously reported the involvement of exosomal vimentin in mediating wound healing. Here we performed in vitro and in vivo experiments to explore the effect of wide-type and vimentin knockout exosomes in accelerating wound healing under osmotic stress condition. Our results showed that osmotic stress increases the size and enhances the release of exosomes. Furthermore, our findings revealed that exosomal vimentin enhances wound healing by protecting fibroblasts against osmotic stress and inhibiting stress-induced apoptosis. These data suggest that exosomes could be considered either as a stress modifier to restore the osmotic balance or as a conveyer of stress to induce osmotic stress-driven conditions.

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“… 41 , 99 , 100 In addition to determination of size and localization of the fluorescently labeled EVs, STED can also be used for morphological characterization of EVs, as well as examining the distribution of the labeled proteins on the EV surface. 101 , 102 Direct stochastic optical reconstruction microscopy (dSTORM) has also been employed to characterize EVs [ Fig. 1(c) ], 41 but this technique is limited to fluorophores with photoactivation and blinking properties.…”

Section: Extracellular Vesicle Characterization Methodsmentioning

confidence: 99%

Overview of extracellular vesicle characterization techniques and introduction to combined reflectance and fluorescence confocal microscopy to distinguish extracellular vesicle subpopulations

et al. 2022

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. Extracellular vesicles (EVs) are nanoparticles (30 to 1000 nm in diameter) surrounded by a lipid-bilayer which carry bioactive molecules between local and distal cells and participate in intercellular communication. Because of their small size and heterogenous nature they are challenging to characterize. Here, we discuss commonly used techniques that have been employed to yield information about EV size, concentration, mechanical properties, and protein content. These include dynamic light scattering, nanoparticle tracking analysis, flow cytometry, transmission electron microscopy, atomic force microscopy, western blotting, and optical methods including super-resolution microscopy. We also introduce an innovative technique for EV characterization which involves immobilizing EVs on a microscope slide before staining them with antibodies targeting EV proteins, then using the reflectance mode on a confocal microscope to locate the EV plane. By then switching to the microscope’s fluorescence mode, immunostained EVs bearing specific proteins can be identified and the heterogeneity of an EV preparation can be determined. This approach does not require specialist equipment beyond the confocal microscopes that are available in many cell biology laboratories, and because of this, it could become a complementary approach alongside the aforementioned techniques to identify molecular heterogeneity in an EV preparation before subsequent analysis requiring specialist apparatus.

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Exosomal vimentin from adipocyte progenitors accelerates wound healing

Cited by 22 publications

References 44 publications

Review of the Role of Mesenchymal Stem Cells and Exosomes Derived from Mesenchymal Stem Cells in the Treatment of Orthopedic Disease

Review of the Role of Mesenchymal Stem Cells and Exosomes Derived from Mesenchymal Stem Cells in the Treatment of Orthopedic Disease

Exosomal Vimentin from Adipocyte Progenitors Protects Fibroblasts against Osmotic Stress and Inhibits Apoptosis to Enhance Wound Healing

Overview of extracellular vesicle characterization techniques and introduction to combined reflectance and fluorescence confocal microscopy to distinguish extracellular vesicle subpopulations

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