Exosomal miR-9-5p secreted by bone marrow–derived mesenchymal stem cells alleviates osteoarthritis by inhibiting syndecan-1

Jin, Zhe; Ren, Jiaan; Qi, Shanlun

doi:10.1007/s00441-020-03193-x

Cited by 71 publications

(42 citation statements)

References 33 publications

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“…Recently, extracellular vesicles (EVs), such as exosomes and microvesicles, have been attracting a strong research interest for use as natural drug delivery systems (Chen et al, 2018;Tang and Wang, 2020). Mesenchymal stem cell (MSC)-derived exosomes have been reported to be involved in multiple physiology and pathology activities including osteogenesis, bone regeneration, osteoarthritis, cardiomyoblast hypoxia-reperfusion, cognitive impairment, inflammation, stroke, sepsis, and tumorigenesis (Zheng et al, 2018;Huang et al, 2020;Jin et al, 2020;Nakano et al, 2020;Tan et al, 2020;Venkat et al, 2020;Xu et al, 2020;Zou et al, 2020). Jia et al (2020) report that exosomes secreted by young MSCs promote osteogenesis and bone formation in older rats.…”

Section: Introductionmentioning

confidence: 99%

BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis

Peng

Liu

et al. 2020

Front. Cell Dev. Biol.

115

103

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Angiogenesis and osteogenesis are tightly coupled during bone modeling and remodeling processes. Here we reported that bone marrow mesenchymal stem cell (BMSC)-derived exosomal miR-29a promotes angiogenesis and osteogenesis in vitro and in vivo. BMSC-derived exosomes (BMSCs-Exos) can be taken up by human umbilical vein endothelial cells (HUVECs) and promote the proliferation, migration, and tube formation of HUVECs. MiRNA-29a level was high in BMSCs-Exos and can be transported into HUVECs to regulate angiogenesis. VASH1 was identified as a direct target of miR-29a, mediating the effects of BMSC-derived exosomal miR-29a on angiogenesis. More interestingly, miR29a-loaded exosomes from engineered BMSCs (miR-29a-loaded BMSCs-Exos) showed a robust ability of promoting angiogenesis and osteogenesis in vivo. Taken together, these findings suggest that BMSC-derived exosomal miR-29a regulates angiogenesis and osteogenesis, and miR-29a-loaded BMSCs-Exos may serve as a potential therapeutic target for osteoporosis.

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Section: Introductionmentioning

confidence: 99%

BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis

Peng

Liu

et al. 2020

Front. Cell Dev. Biol.

115

103

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“…For this investigation into the inter‐relationships between resistin and pro‐inflammatory mediators IL‐1β and TNF‐α during OA pathogenesis, we selected the ACLT OA model as being the most appropriate surgical OA animal model for revealing resistin‐induced molecular changes in the cartilage, synovial tissue, and subchondral bone, all of which can be studied within a short timeframe, as illustrated in our previous study 21,40 . It is well recognized that OA disease progression is closely associated with progressive destruction of articular cartilage, subchondral bone remodeling and inflammatory changes in the synovial membrane although the etiology and pathogenesis of OA is less clear 41 . The present study demonstrated that injection of resistin shRNA into rats with OA‐like damage alleviated cartilage injury and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Resistin enhances IL‐1β and TNF‐α expression in human osteoarthritis synovial fibroblasts by inhibiting miR‐149 expression via the MEK and ERK pathways

Chen

Kuo

et al. 2020

FASEB j.

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Resistin is a cysteine‐rich adipokine that promotes the release of inflammatory cytokines, particularly interleukin 1 beta (IL‐1β) and tumor necrosis factor alpha (TNF‐α), which are critical pro‐inflammatory mediators in osteoarthritis (OA) pathogenesis. We describe evidence of significantly higher levels of resistin, IL‐1β, and TNF‐α expression in OA knee synovial tissue compared with that from non‐OA knees. Resistin‐induced enhancement of IL‐1β and TNF‐α expression in human OA synovial fibroblasts (OASFs) were attenuated by MEK and ERK inhibitors, as well as their respective siRNAs. Our data reveal that resistin enhances the expression of TNF‐α and IL‐1β in OASFs by inhibiting miR‐149 expression via MEK and ERK signaling. Our findings elucidate the inter‐relationships between resistin and pro‐inflammatory mediators during OA pathogenesis and could help to facilitate the development of synovium‐targeted therapy in OA.

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“…inhibited the in ammatory response and repaired injured cartilage 18,19 . Small RNAs in exosomes decrease the expression of in ammatory factors and improve oxidative stress states 20,21 . The next steps are the targeting of exosomes and the identi cation of small RNAs that speci cally regulate the expression of TGF-β, which would improve the effectiveness of treatment.…”

Section: Discussionmentioning

confidence: 99%

The 100 Most Cited Articles on the Applications of Stem Cells in Osteoarthritis: A Bibliometric Analysis

Yang¹,

Zhou²,

Lin

2020

Preprint

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Background: Stem cells have been applied in the treatment of OA, which had attracted wide attention. However, the research area is relatively extensive, and the research level is variable. In this study, we reviewed the mechanisms and clinical applications of stem cells in OA by using bibliometric analysis for the first time. We also revealed the characteristics, superior results and developmental trends in this field.Methods: The Web of Science core collection database was used to search articles related to the application of stem cells in OA. We collected the general information from the top 100 cited articles. We analyzed and evaluated the articles according to publication number, journals, institutions, countries, keywords and extended keywords.Results: The 100 most cited articles were cited from 129 to 1353 times mainly reviews and original articles. These articles were published from 2001 to 2017 and distributed evenly in America, East Asia and European countries. The United States contributed most in published number and international cooperation. The top ten institutions are mainly major universities and Duke University published a maximum of 10 articles. In terms of journals ,57 articles were published in the top ten journals. The keywords were divided into 8 categories from molecular mechanisms to clinical application.Conclusions: In our study, we found that mesenchymal stem cells (MSCs) which could repair articular cartilage and inhibit local inflammation, are the most widely applied in research and treatment of OA. TGF-βwas crucial during the process. Exosomes are regarded as the active ingredients in stem cell therapy for OA. Microtissue engineering will contribute to accurate and effective stem cell therapy. The findings of our study will contribute to the continuous development of research and direct the research of stem cells in OA.

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Exosomal miR-9-5p secreted by bone marrow–derived mesenchymal stem cells alleviates osteoarthritis by inhibiting syndecan-1

Cited by 71 publications

References 33 publications

BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis

BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis

Resistin enhances IL‐1β and TNF‐α expression in human osteoarthritis synovial fibroblasts by inhibiting miR‐149 expression via the MEK and ERK pathways

The 100 Most Cited Articles on the Applications of Stem Cells in Osteoarthritis: A Bibliometric Analysis

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