Exosomal microRNAs in breast cancer and their potential in diagnosis, prognosis and treatment prediction

Faraji, Ghazale; Moeini, Pedram; Ranjbar, Mohammad Hasan

doi:10.1016/j.prp.2022.154081

Cited by 5 publications

(4 citation statements)

References 54 publications

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“…The underlying molecular mechanism of PTX-resistance in BC remains to be elucidated. Cir-cRNA is a special circular single-strand RNA produced by precursor mRNA back-splicing, which plays roles in serving as miRNAs sponges, RNA binding proteins sponges, proteins/peptide translators, and transcription regulators [51][52][53][54][55][56]. In this study, we found that the expression of circBACH1 was significantly increased in PTX-EXO of MCF-7 and MDA-MB-231.…”

Section: Discussionmentioning

confidence: 86%

“…MiRNAs are small non-coding RNAs around 20–24 bps, which involve in cell cycle including cell proliferation, differentiation, and death [ 54 ]. MiRNAs regulate transcription, translation, and degradation of mRNAs to mediate target genes [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…MiRNAs regulate transcription, translation, and degradation of mRNAs to mediate target genes [55]. Massive miRNAs have been reported to be promising biomarkers for BC metastasis such as lymph node metastasis, bone metastasis, brain metastasis, and distant metastasis [39,40,54]. Previous studies showed that miR-217 inhibited the proliferation, invasion, and migration of several cancer cells including prostate cancer cells, pancreatic cancer cells, and pulmonary carcinoma cells, due to the suppression of target gene expression and relative signaling pathway [32][33][34].…”

Section: Discussionmentioning

confidence: 99%

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Chemotherapy-induced exosomal circBACH1 promotes breast cancer resistance and stemness via miR-217/G3BP2 signaling pathway

et al. 2023

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Background Chemoresistance involves metastasis and aggressiveness of breast cancer (BC). Chemotherapy-elicited exosomes have been reported to be associated with drug resistance and pro-metastatic capacity of BC cells. Non-coding RNAs (ncRNAs) are enriched in exosomes, which participated in generation, progression, and resistance of BC. However, the mechanism underlying the chemoresistance and metastasis in BC cells mediated by the BC-derived exosomal ncRNAs remained to be elucidated. Methods The effects of PTX-induced exosomal circBACH1 on BC cell function were assessed using RNA Binding Protein Immunoprecipitation (RIP), dual luciferase reporter gene, tube formation, CCK-8, and Western Blot assays. The circBACH1 and miR-217 expression levels were detected using quantitative real-time PCR (RT-qPCR) and Immunohistochemistry (IHC) assays in BC tissues and precancerous tissues of BC patients. Results CircBACH1 expression was increased in paclitaxel-treated BC-derived exosomes (PTX-EXO) and BC tissue. PTX-EXO was shown to promote PTX-resistance and angiogenesis through upregulation circBACH1. Downregulation of circBACH1 improved PTX-sensitiveness by suppressing the cell viability, stemness, migration, and angiogenesis of BC cells. Moreover, we found that miR-217 interacted with circBACH1 and targeted GTPase-activating SH3 domain-binding protein 2 (G3BP2) in BC cells. CircBACH1 combined miR-217 cotransfection suppressed the expression of G3BP2 proteins compared with circBACH1 treatment in MCF-7 cells. In addition, downregulation of G3BP2 suppressed BC cell migration. Conclusions These results demonstrated that PTX-induced exosomal circBACH1 promoted stemness and migration of BC cells by sponging miR-217 to upregulate the expression of G3BP2, which provided a new therapeutic target for PTX-resistance and progression of BC via circBACH1/miR-217/G3BP2 axis.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Chemotherapy-induced exosomal circBACH1 promotes breast cancer resistance and stemness via miR-217/G3BP2 signaling pathway

et al. 2023

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“…In addition to their diagnostic value, exosomal miRNAs have been extensively studied in the prognosis of cancers [ 97 ]. Downregulation of serum exosomal miR-148a was associated to a worse clinical outcome in breast cancer patients.…”

Section: Prognostic Biomarkermentioning

confidence: 99%