Exosomal microRNA-150-5p from bone marrow mesenchymal stromal cells mitigates cerebral ischemia/reperfusion injury via targeting B-cell translocation gene 2

Abstract

Objective MicroRNA(miR)-150-5p has been investigated in many studies, while the role of exosomal miR-150-5p from bone marrow mesenchymal stromal cells (BMSCs) on cerebral ischemia/reperfusion (I/R) injury remains extensive exploration. This research aims to probe the protective effects of exosomal miR-150-5p from BMSCs on cerebral I/R injury via regulating B-cell translocation gene 2 (BTG2). Methods BMSCs were cultured and transfected with miR-150-5p mimic, then exosomes from BMSCs were extracted. Middle cerebral artery occlusion (MCAO) rat model was established, and miR-150-5p and BTG2 levels in rat brain tissues were detected. Then gain and loss-function assays were conducted to probe the impact of exosomes, miR-150-5p and BTG2 on neurological function, pathological changes, apoptosis and inflammatory factors of MCAO rats. The binding relationship between miR-150-5p and BTG2 was validated. Results MiR-150-5p was decreased and BTG2 was augmented in MCAO rats. The exosomes from BMSCs could improve neurological function, pathological changes, apoptosis and reduce inflammatory factors in MCAO rats. Enriched miR-150-5p or decreased BTG2 could enhance the protective effects of exosomes from BMSCs on cerebral I/R injury. The elevated BTG2 reversed the impacts of enriched exosomal miR-150-5p. BTG2 was targeted by miR-150-5p. Conclusion Exosomal miR-150-5p from BMSCs exerts protective effects on cerebral I/R injury via repressing BTG2. This study provided novel therapeutic strategies for treatment of cerebral I/R injury.