Exosomal MicroRNA-126 from RIPC Serum Is Involved in Hypoxia Tolerance in SH-SY5Y Cells by Downregulating DNMT3B

Cui, Jun-He; Liu, Na; Chang, Zhe-Han; Gao, Yongsheng; Bao, Mulan; Xie, Yandong; Wei, Xu; Liu, Xiaolei; Jiang, Shuyuan; Li, You; Shi, Rui; Xie, Wei; Jia, Xiaoe; Shi, Jinghua; Ren, Chang­hong; Gong, Kerui; Zhang, Chunyang; Bade, Rengui; Shao, Guo; Ji, Xunming

doi:10.1016/j.omtn.2020.04.008

Cited by 32 publications

(23 citation statements)

References 49 publications

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“…32 Additionally, exosomal microR-NA-126 from the venous blood of remote ischemic preconditioning was found to induce hypoxia tolerance of SH-SY5Y cells via targeting DNMT3B. 33 Collectively, these studies suggest that miRNAs plays important roles in HIE. Notwithstanding, the clinical therapeutic effects of miRNAs on human diseases have rarely been reported, and the roles of miRNAs have primarily been elucidated using cells or animal models.…”

Section: Discussionmentioning

confidence: 96%

Overexpression of miR-146b-5p Ameliorates Neonatal Hypoxic Ischemic Encephalopathy by Inhibiting IRAK1/TRAF6/TAK1/NF-αB Signaling

Yang

Zhao

2020

Yonsei Med J

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Purpose: Neonatal hypoxic ischemic encephalopathy (HIE) is an essential factor underlying neonatal death and disability. This study sought to explore the role of miR-146b-5p in regulating neonatal HIE. Materials and Methods: In vitro and in vivo HIE models were established in PC12 cells and 10-day neonatal Sprague Dawley rats, respectively. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to assess miR-146b-5p expression and inflammatory factors [interleukin (IL)-6 and tumor necrosis factor (TNF)-α] in brain lesions and PC12 cells, while enzymelinked immunosorbent assay was employed to detect the expression of oxidative stress factors (SOD and GSH-Px). Gain-and loss-assays of miR-146b-5p were conducted to verify its role in modulating the viability and apoptosis of PC12 cells under oxygenglucose deprivation (OGD) treatment. Expression of TLR4, IRAK1, TRAF6, TAK1, and NF-κB were examined by qRT-PCR and/or Western blot. Dual luciferase activity assay was conducted to identify relationships between miR-146b-5p and IRAK1. Results: In the HIE models, significant oxidative stress and inflammatory responses emerged upon upregulation of TLR4/IRAK1/ TRAF6/TAK1/NF-κB signaling. Overexpression of miR-146b-5p greatly inhibited OGD-induced PC12 cell injury, inflammatory responses, and oxidative stress. Inhibiting miR-146b-5p, however, had the opposite effects. IRAK1 was found to be a target of miR-146b-5p, and miR-146b-5p overexpression suppressed the activation of IRAK1/TRAF6/TAK1/NF-κB signaling. Conclusion: This study demonstrated that miR-146b-5p overexpression alleviates HIE-induced neuron injury by inhibiting the IRAK1/TRAF6/TAK1/NF-κB pathway.

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Section: Discussionmentioning

confidence: 96%

Overexpression of miR-146b-5p Ameliorates Neonatal Hypoxic Ischemic Encephalopathy by Inhibiting IRAK1/TRAF6/TAK1/NF-αB Signaling

Yang

Zhao

2020

Yonsei Med J

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“…SH-SY5Y has been widely used as a research object of PD because it has a mechanism to synthesize DA (activate dopamineβ-hydroxylase) and NA (Xicoy et al, 2017). But not limited to this, it is also widely used in other areas of neurological disorders, including AD (de Medeiros et al, 2019), neurotoxicity (Wang et al, 2019), glutamate excitotoxicity (Vucicevic et al, 2020), ischemia (Cui et al, 2020) or amyotrophic lateral sclerosis (ALS) (Giannini et al, 2020) and other diseases. However, SH-SY5Y retains the characteristics of cancer including the instability of the proliferation, differentiation, and metabolism because it is isolated from neuroblastoma derivatives.…”

Section: Sh-sy5y Cell Linesmentioning

confidence: 99%

Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders

et al. 2021

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Cell cultures are used in pharmaceutical, medical and biological sciences. Due to the ethical and cost limitations of in vivo models, the replaceable cell model that is more closely related to the characteristics of organisms, which has broad prospects and can be used for high-throughput drug screening is urgent. Neuronal and glial cell models have been widely used in the researches of neurological disorders. And the current researches on neuroinflammation contributes to blood-brain barrier (BBB) damage. In this review, we describe the features of healthy and inflamed BBB and summarize the main immortalized cell lines of the central nervous system (PC12, SH-SY5Y, BV2, HA, and HBMEC et al.) and their use in the anti-inflammatory potential of neurological disorders. Especially, different co-culture models of neuroinflammatory, in association with immune cells in both 2D and 3D models are discussed in this review. In summary, 2D co-culture is easily practicable and economical but cannot fully reproduce the microenvironment in vivo. While 3D models called organs-on-chips or biochips are the most recent and very promising approach, which made possible by bioengineering and biotechnological improvements and more accurately mimic the BBB microenvironment.

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“…Another investigation explored if exosomal miRNA-126 from RIPC serum may have neuro-protective role [ 252 ]. Researchers isolated the exosomes from the venous serum of 4 healthy-young male cases prior to and following RIPC.…”

Section: Exosomal Micrornas and Strokementioning

confidence: 99%

“…Following the miRNA-126 over-expression in the SH-SY5Y cells, the overall methylation level as well as DNMT3B gene expression have been down-regulated in the above cells that has been compatible with the bio-informatics prediction. Finally, RIPC exosomes are capable of influencing the cell-cycle and increasing the tolerance of OGD in the SH-SY5Y cells and apparently RIPC has neuro-protective effects by down-regulating DNMTs expression in the neural cells via upregulating the serum exosomal miRNA-126 [ 252 ]. Table 3 lists exosomal miRNAs that are involved in stroke.…”

Section: Exosomal Micrornas and Strokementioning

confidence: 99%

New epigenetic players in stroke pathogenesis: From non-coding RNAs to exosomal non-coding RNAs

Mahjoubin‐Tehran

Rezaei

Jesmani

et al. 2021

Biomedicine & Pharmacotherapy

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Non-coding RNAs (ncRNAs) have critical role in the pathophysiology as well as recovery after ischemic stroke. ncRNAs, particularly microRNAs, and the long non-coding RNAs (lncRNAs) are critical for angiogenesis and neuroprotection, and they have been suggested to be therapeutic, diagnostic and prognostic tools in cerebrovascular diseases, including stroke. Moreover, exosomes have been considered as nanocarriers capable of transferring various cargos, such as lncRNAs and miRNAs to recipient cells, with prominent inter-cellular roles in the mediation of neuro-restorative events following strokes and neural injuries. In this review, we summarize the pathogenic role of ncRNAs and exosomal ncRNAs in the stroke.

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Exosomal MicroRNA-126 from RIPC Serum Is Involved in Hypoxia Tolerance in SH-SY5Y Cells by Downregulating DNMT3B

Cited by 32 publications

References 49 publications

Overexpression of miR-146b-5p Ameliorates Neonatal Hypoxic Ischemic Encephalopathy by Inhibiting IRAK1/TRAF6/TAK1/NF-αB Signaling

Overexpression of miR-146b-5p Ameliorates Neonatal Hypoxic Ischemic Encephalopathy by Inhibiting IRAK1/TRAF6/TAK1/NF-αB Signaling

Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders

New epigenetic players in stroke pathogenesis: From non-coding RNAs to exosomal non-coding RNAs

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