Exosomal microRNA-125a-3p from human adipose-derived mesenchymal stem cells promotes angiogenesis of wound healing through inhibiting PTEN

Pi, Li; Yang, Li; Fang, Bairong; Meng, Xianxi; Li, Qian

doi:10.1007/s11010-021-04251-w

Cited by 35 publications

(27 citation statements)

References 38 publications

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“…Other microRNAs carried by ADSC-EVs were also reported to participate in angiogenesis, such as miR-126, miR-130a, and miR-132 ( 75 ). Additionally, miR-125a-3p from ADSC-EVs enhanced angiogenesis of HUVECs by activating the PI3K/AKT signaling pathway, targeting the PTEN gene ( 76 ). Another study indicated that ADSC-EVs also contributed to angiogenesis in the diabetes microenvironment by increasing HIF-1α and VEGF expression through the PI3K/AKT/mTOR signaling pathway ( 77 ).…”

Section: Mechanisms By Which Adsc-evs Play a Therapeutic Role In Skin...mentioning

confidence: 99%

The Therapeutic Role of ADSC-EVs in Skin Regeneration

et al. 2022

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Large skin defects caused by burns, unhealing chronic wounds, and trauma, are still an intractable problem for clinicians and researchers. Ideal skin regeneration includes several intricate and dynamic stages of wound repair and regeneration of skin physiological function. Adipose-derived stem cells (ADSCs), a type of mesenchymal stem cells (MSCs) with abundant resources and micro-invasive extraction protocols, have been reported to participate in each stage of promoting skin regeneration via paracrine effects. As essential products secreted by ADSCs, extracellular vesicles (EVs) derived from ADSCs (ADSC-EVs) inherit such therapeutic potential. However, ADSC-EVs showed much more clinical superiorities than parental cells. ADSC-EVs carry various mRNAs, non-coding RNAs, proteins, and lipids to regulate the activities of recipient cells and eventually accelerate skin regeneration. The beneficial role of ADSCs in wound repair has been widely accepted, while a deep comprehension of the mechanisms of ADSC-EVs in skin regeneration remains unclear. In this review, we provided a basic profile of ADSC-EVs. Moreover, we summarized the latest mechanisms of ADSC-EVs on skin regeneration from the aspects of inflammation, angiogenesis, cell proliferation, extracellular matrix (ECM) remodeling, autophagy, and oxidative stress. Hair follicle regeneration and skin barrier repair stimulated by ADSC-EVs were also reviewed. The challenges and prospects of ADSC-EVs-based therapies were discussed at the end of this review.

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Section: Mechanisms By Which Adsc-evs Play a Therapeutic Role In Skin...mentioning

confidence: 99%

The Therapeutic Role of ADSC-EVs in Skin Regeneration

et al. 2022

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“…Mechanistically, they propose that miR-125a, enriched in exosomes, repressed the expression of angiogenic inhibitor delta-like 4 (DLL4), resulting in enhanced angiogenesis. The finding of miR-125a in exosomes produced by adipose tissue progenitor cells, and its pro-angiogenic effect on human umbilical endothelial cells was confirmed by Pi et al, [133] who also find an effect of these exosomes to inhibit PTEN. In other contexts, miR-125 has been shown to have anti-angiogenic effects [134][135][136], for example, deletion of miR-125a in zebrafish resulted in a hyper-branching phenotype [134].…”

Section: Mechanisms Of Inter-cellular Communicationmentioning

confidence: 68%

Angiogenesis in adipose tissue and obesity

2022

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While most tissues exhibit their greatest growth during development, adipose tissue is capable of additional massive expansion in adults. Adipose tissue expandability is advantageous when temporarily storing fuel for use during fasting, but becomes pathological upon continuous food intake, leading to obesity and its many comorbidities. The dense vasculature of adipose tissue provides necessary oxygen and nutrients, and supports delivery of fuel to and from adipocytes under fed or fasting conditions. Moreover, the vasculature of adipose tissue comprises a major niche for multipotent progenitor cells, which give rise to new adipocytes and are necessary for tissue repair. Given the multiple, pivotal roles of the adipose tissue vasculature, impairments in angiogenic capacity may underlie obesity-associated diseases such as diabetes and cardiometabolic disease. Exciting new studies on the single-cell and single-nuclei composition of adipose tissues in mouse and humans are providing new insights into mechanisms of adipose tissue angiogenesis. Moreover, new modes of intercellular communication involving micro vesicle and exosome transfer of proteins, nucleic acids and organelles are also being recognized to play key roles. This review focuses on new insights on the cellular and signaling mechanisms underlying adipose tissue angiogenesis, and on their impact on obesity and its pathophysiological consequences.

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“…Therefore, IL-6 might regulate ESCRT (endosomal sorting complex required for transport) in HCC cells and load specific microRNAs (miR-133a-3p/miR-199a-5p/miR-143-3p) into exosomes. The microRNAs released from HCC cells could also be taken in by other cells in the tumor microenvironment like macrophages, endothelial cells, and tumor-associated fibroblasts for more functions such as immune escape [ 28 ] and angiogenesis [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

IL-6 Promotes Hepatocellular Carcinoma Invasion by Releasing Exosomal miR-133a-3p

Ren

Zhou

Luo

et al. 2022

Gastroenterology Research and Practice

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Interleukin-6 (IL-6), an important inflammatory cytokine, is a key factor regulating cancer metastasis. Cancer cells can modulate their tumorigenic abilities by sorting specific microRNAs (miRNAs) as exosomes into the tumor microenvironment. The relationship between IL-6 and exosomal miRNAs related to hepatocellular carcinoma (HCC) metastasis remains to be elucidated. We examined the metastatic ability of HCC cells after IL-6 treatment and found that miR-133a-3p was sorted into exosomes after IL-6 stimulation and was subsequently released into the tumor microenvironment. In vitro analysis confirmed that exosomal miR-133a-3p acted as a tumor suppressor in HCC. Bioinformatic analysis revealed several signaling pathways and hub genes (CREB1, VCP, CALM1, and YES1) regulated by miR-133a-3p. Survival curves further verified the important roles of hub genes in the prognosis of patients with HCC. It is envisaged that the IL-6/miR-133a-3p axis may be related to the activation of CREB1, VCP, CALM1, and YES1. Our findings provide new insights into the role of exosomal miRNA-mediated tumor progression under inflammatory conditions.

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Exosomal microRNA-125a-3p from human adipose-derived mesenchymal stem cells promotes angiogenesis of wound healing through inhibiting PTEN

Cited by 35 publications

References 38 publications

The Therapeutic Role of ADSC-EVs in Skin Regeneration

The Therapeutic Role of ADSC-EVs in Skin Regeneration

Angiogenesis in adipose tissue and obesity

IL-6 Promotes Hepatocellular Carcinoma Invasion by Releasing Exosomal miR-133a-3p

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