Exosomal circFBLIM1 Promotes Hepatocellular Carcinoma Progression and Glycolysis by Regulating the miR-338/LRP6 Axis

Lai, Zhiwen; Wei, Tianning; Li, Qingming; Wang, Xianglong; Zhang, Yang; Zhang, Shengliang

doi:10.1089/cbr.2020.3564

Cited by 38 publications

(34 citation statements)

References 36 publications

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“…The observation of high circ-FBLIM1 levels in HCC patients led the authors to hypothesize a relevant role for this circRNA in tumors. Their findings verified that circFBLIM1 contributed to the progression and glycolysis of HCC by sponging miR-338 and determining the consequent upregulating of LDL Receptor Related Protein 6 (LRP6) [ 100 ]. Despite the evidence of the high levels of circ-FBLIM1 in HCC exosomes, its value as a tumor biomarker should be further validated.…”

Section: Exosomal-derived Lncrnas and Circrnasmentioning

confidence: 85%

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Circulating Long and Circular Noncoding RNA as Non-Invasive Diagnostic Tools of Hepatocellular Carcinoma

et al. 2021

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Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-related death worldwide, partially due to late diagnosis of the disease. Growing evidence in the field of biomarker discovery has shown the promising use of nucleic acid in the early detection of many cancers, including HCC. Here, we review data on how various long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) could be used as a diagnostic tool for HCC being differentially expressed in HCC compared to non-HCC patients. These non-coding RNAs (ncRNAs) showed high stability in the blood being present as free-circulating molecules or encapsulated into exosomes. This review reports some recent evidence on the use of lncRNAs and circRNAs as possible diagnostic biomarkers for HCC. Further, their pathophysiological mechanism in liver carcinogenesis was also described, elucidating the complex regulatory networks making these ncRNAs of particular relevance for the study of liver malignancy cancer.

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Section: Exosomal-derived Lncrnas and Circrnasmentioning

confidence: 85%

“…Likewise, the expression of circ-FBLIM1 was investigated in 30 HCC samples compared to 25 healthy volunteers by Lai and colleagues [ 100 ]. The observation of high circ-FBLIM1 levels in HCC patients led the authors to hypothesize a relevant role for this circRNA in tumors.…”

Section: Exosomal-derived Lncrnas and Circrnasmentioning

confidence: 99%

Circulating Long and Circular Noncoding RNA as Non-Invasive Diagnostic Tools of Hepatocellular Carcinoma

et al. 2021

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“…Due to the unique circular closed structure, high stability, and special expression patterns, it is believed that circRNAs can become potential biomarkers (25). In fact, circRNAs are dysregulated in various cancers, including LUACs (26)(27)(28). Several studies have suggested that dysregulated circRNAs in tissues or plasma could be used as novel biomarkers for cancer (29,30).…”

Section: Discussionmentioning

confidence: 99%

The roles and mechanisms of the circular RNA circ_104640 in early-stage lung adenocarcinoma: a potential diagnostic and therapeutic target

et al. 2021

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Background: In recent years, there have been increasing reports that dysregulated circular RNAs (circRNAs) play a key role in the carcinogenesis of lung adenocarcinoma (LUAC). However, the role of circRNAs in early-stage LUAC is poorly understood. Methods:The Gene Expression Omnibus (GEO) database and qRT-PCR were used to verify the abnormal expression of circRNAs, miRNAs and genes in early-stage LUAC tissues. shRNA and miRNA inhibitor are designed and synthesized to knock down circ_104640 and microRNA (miR)-145-5p expression.CCK-8 assay, colony formation assay and flow cytometry were used to study the effect of circ_104640 on cell proliferation and apoptosis. Bioinformatics analysis, dual luciferase reporter assays and argonaute 2 (Ago2) RNA immunoprecipitation (RIP) assays were chosen to find out the potential target of circ_104640.Results: Based on the GEO database and tissue sample from our institution, we identified that the circRNA circ_104640, the miR-145-5p, and CCL20 (C-C motif chemokine ligand 20) were abnormally expressed in the tissues of early-stage LUAC. In vitro experiments showed that circ_104640 could exist stably in the cytoplasm, and a short pin RNA that targeted circ_104640 (sh-circ) inhibited cell growth and promoted apoptosis of LUAC cells. Dual luciferase reporter assays and Ago2 (RIP) assays confirmed the Ago2-dependent interaction of circ_104640 and miR-145-5p. In terms of mechanisms, we found that circ_104640 increased the expression of CCL20 by sponging miR-145-5p.Conclusions: Our research demonstrated that circ_104640 could accelerate the proliferation of LUAC cells, while inhibiting LUAC cell apoptosis. circ_104640 may be a potential novel biomarker and therapeutic target for early-stage LUAC.

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“…Several studies have demonstrated that EVs could directly regulate the growth of HCC through different pathways [ 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ]. Gai et al found that a serum protein marker involved in the tumorigenesis and metastasis of HCC, Golgi membrane protein 1 (GOLM1), was significantly enriched in HCC-derived EVs.…”

Section: Evs As Mediators Of Chronic Liver Disease and Hcc Progressionmentioning

confidence: 99%

The Role of Extracellular Vesicles in Disease Progression and Detection of Hepatocellular Carcinoma

Lee

Tran

Wang

et al. 2021

Cancers

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Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and one of the leading causes of cancer-related death worldwide. Despite the improvements in surveillance and treatment, the prognosis of HCC remains poor. Extracellular vesicles (EVs) are a heterogeneous group of phospholipid bilayer-enclosed particles circulating in the bloodstream and mediating intercellular communication. Emerging studies have shown that EVs play a crucial role in regulating the proliferation, immune escape, and metastasis of HCC. In addition, because EVs are present in the circulation at relatively early stages of disease, they are getting attention as an attractive biomarker for HCC detection. Over the past decade, dedicated efforts have been made to isolate EVs more efficiently and make them useful tools in different clinical settings. In this review article, we provide an overview of the EVs isolation methods and highlight the role of EVs as mediators in the pathogenesis and progression of HCC. Lastly, we summarize the potential applications of EVs in early-stage HCC detection.

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Exosomal circFBLIM1 Promotes Hepatocellular Carcinoma Progression and Glycolysis by Regulating the miR-338/LRP6 Axis

Cited by 38 publications

References 36 publications

Circulating Long and Circular Noncoding RNA as Non-Invasive Diagnostic Tools of Hepatocellular Carcinoma

Circulating Long and Circular Noncoding RNA as Non-Invasive Diagnostic Tools of Hepatocellular Carcinoma

The roles and mechanisms of the circular RNA circ_104640 in early-stage lung adenocarcinoma: a potential diagnostic and therapeutic target

The Role of Extracellular Vesicles in Disease Progression and Detection of Hepatocellular Carcinoma

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