Exopolysaccharides produced by the recently described halophilic bacteria Halomonas ventosae and Halomonas anticariensis

Mata, Juan Antonio; Béjar, Victoria; Llamas, Inmaculada; Arias, Soledad; Bressollier, Philippe; Tallon, Richard; Urdaci, María C.; Quesada, Emilia

doi:10.1016/j.resmic.2006.06.004

Cited by 151 publications

(127 citation statements)

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“…Halomonas anticariensis FP35 T was first identified in saline soils at Fuente de Piedra, Málaga, in southern Spain. This bacterium characteristically grows over a wide salt range of 0.5 % to 15 % w/v and excretes significant quantities of EPS when the early stationary growth phase is reached (Mata et al, 2006). We have reported that this bacterium produces a series of AHLs of many sizes [C 4 -homoserine lactone (HSL), C 6 -HSL, C 8 -HSL and C 12 -HSL] and have recently identified and characterized the QS genes hanR/hanI involved in their production (Tahrioui et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic and phenotypic analysis of the GacS/GacA system in the moderate halophile Halomonas anticariensis

2013

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A multisensory, hybrid histidine kinase (HK) and a response regulator (RR), which together may well constitute a two-component regulatory system (TCS), have been located in Halomonas anticariensis FP35 T by transposon mutagenesis. This TCS is homologous to the GacS/GacA system described for many Gram-negative bacteria. An analysis of crude N-acylhomoserine lactone (AHL) extracts from cultures of FP35gacS and FP35gacA mutants showed that they produced lower quantities of AHLs than the wild-type strain. In addition, RT-PCR analysis revealed a considerable decrease in the expression of the quorum-sensing (QS) genes hanR and hanI compared with the wild-type strain. This result indicates that the GacS/GacA TCS exerts a positive effect upon the QS HanR/HanI system and suggests its integral involvement in the intercellular communication strategies of this bacterium. We have also demonstrated the influence of GacS and GacA upon exopolysaccharide production and biofilm formation, in which this regulatory machinery appears to play a key role in an overall system that co-ordinates gene expression and behaviour in H. anticariensis FP35 T in response to environmental conditions.

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Section: Introductionmentioning

confidence: 99%

Genetic and phenotypic analysis of the GacS/GacA system in the moderate halophile Halomonas anticariensis

2013

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“…They are widely distributed throughout hypersaline environments. Some of them are recognized for their potential use in biotechnology (Margesin & Schinner, 2001;Ventosa & Nieto, 1995), producing exopolysaccharides (Arias et al, 2003;Mata et al, 2006;Martínez-Checa et al, 2002), denitrifying (Peyton et al, 2001;Yoshie et al, 2006) or degrading aromatic compounds (García et al, 2004). …”

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Halomonas fontilapidosi sp. nov., a moderately halophilic, denitrifying bacterium

González-Domenech

Martínez‐Checa

Quesada

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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We have made a polyphasic taxonomic study of strain 5CR T , isolated from Fuente de Piedra, Má laga, southern Spain. The strain is a moderately halophilic, Gram-negative rod, oxidase-positive and motile by a single polar flagellum. It does not produce acids from sugars and shows respiratory metabolism, using oxygen, nitrate and nitrite as terminal electron acceptors. It requires NaCl and grows best with 5-7.5 % w/v at temperatures of between 32 and 45 6C within a pH range of 6-8. Its 16S rRNA gene sequence indicates that strain 5CR T belongs to the genus Halomonas in the class Gammaproteobacteria. Its closest relatives are Halomonas alimentaria, H. nitroreducens, H. shengliensis and H. ventosae, with the type strains of which our strain showed 16S rRNA gene sequence similarity values of 96.7-97.8 %. DNA-DNA hybridization studies between strain 5CR T and H. ventosae CECT 5797 T , the phylogenetically nearest type strain, showed 40 % relatedness. Its G+C content is 65.7 mol%. Its major fatty acids are C 18 : 1 v7c (31.36 %), C 16 : 0 (25.55 %), C 16 : 1 v7c/iso-C 15 : 0 2-OH (23.23 %), C 19 : 0 cyclo v8c (8.14 %), C 12 : 0 3-OH (5.76 %) and C 10 : 0 (2.22 %) and the predominant respiratory lipoquinone is ubiquinone with nine isoprene units (Q-9). The proposed name for the novel species is Halomonas fontilapidosi sp. nov., strain 5CR T (5CECT 7341 T 5LMG 24455 T ) being the type strain.The family Halomonadaceae of the class Gammaproteobacteria comprises seven genera, Carnimonas, Chromohalobacter, Cobetia, Halomonas, Halotalea, Modicisalibacter and Zymobacter (Euzéby, 2008). Chromohalobacter, Cobetia, Halomonas and Modicisalibacter are composed of halophilic bacteria whereas Carnimonas, Halotalea and Zymobacter contain non-halophilic bacteria (Ben Ali Gam et al., 2007;Garrity et al., 2005;Ntougias et al., 2007). The genus Halomonas currently contains more than 50 species (Euzéby, 2008). Its members are Gram-negative, rodshaped, non-sporulated, aerobic chemo-organotrophs, with predominantly respiratory metabolism, using oxygen, nitrate or nitrite as electron acceptors. Few Halomonas species produce acids from sugars (see Mata et al., 2002;Dobson & Franzmann, 1996;Franzmann et al., 1988;Vreeland, 2005;Arahal et al., 2007). They are widely distributed throughout hypersaline environments. Some of them are recognized for their potential use in biotechnology (Margesin & Schinner, 2001;Ventosa & Nieto, 1995), producing exopolysaccharides (Arias et al., 2003;Mata et al., 2006;Martínez-Checa et al., 2002), denitrifying (Peyton et al., 2001; Yoshie et al., 2006) or degrading aromatic compounds (García et al., 2004).We have already described some novel halophilic taxa isolated from Fuente de Piedra (Málaga, Spain): Idiomarina fontislapidosi (Martínez-Cánovas et al., 2004c), Halomonas anticariensis (Martínez-Cánovas et al., 2004b) and strain 15CR of Halomonas cerina (González-Domenech et al., 2008a). Furthering our research into bacteria in this area, we subjected strain 5CR T to a polyphasic taxonomic investigation and as ...

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“…Bunlar arasında Haloferax, Haloarcula, Halococcus, Natronococcus ve Halobacterium türleri bulunur [6]. En yaygın EPS üreten halofilik bakteriler Halomonas ventosae ve Halomonas anticariensis'dir [29]. EPS üreten Halomonas türleri alışılmadık biçimde yüksek sülfat ve önemli miktarda üronik asit üretir ve bu da jelleşme özelliğini belirler [6].…”

Section: Eps üReten Mikroorganizmalar (Exopolysaccharides Producing Munclassified

Mikrobiyel ekzopolisakkaritler

ERGENE¹,

Avcı²

2016

SAUFenBilDer

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ÖZMikrobiyel ekzopolisakkaritler (EPS) birçok mikroorganizma tarafından üretilerek dış ortama salgılanan karbonhidrat yapısındaki biyopolimerlerdir. EPS, mikroorganizmayı kurumadan, fagositozdan, faj etkisinden korur, ısı, ışık, ses gibi stres ortamında bariyer görevi görür. Endüstriyel amaçla üretilen EPS'ler genellikle gıda, kozmetik, petrol, kimya gibi alanlarda kullanılmaktadır. Mikrobiyel ekzopolisakkaritler gıdalarda stabilizatör, emülgatör, jelleştirme ajanı, nem tutucu, kıvam verici gibi amaçlarla kullanılmaktadır. Bu amaç için en çok kullanılan mikrobiyel ekzopolisakkaritlerin başında ksantan ve jellan gelmektedir. Bunların yanında, dekstran, kurdlan, levan, pullulan, bakteriyel aljinatlar gibi önemli özelliklere sahip EPS'lerin kısıtlı da olsa kullanımları mevcuttur. Bu derlemede, mikrobiyel EPS'lerin özellikleri ve önemi üzerinde durulmuştur. Anahtar Kelimeler: EPS, mikrobiyel ekzopolisakkritler, KsantanMicrobial exopolisaccharides ABSTRACT Microbial exopolysaccharides (EPSs) are carbohydrate biopolymers that are produced and secreted by various microorganisms into their surroundings. EPS protects the microorganism against drying, phagocytosis and phage attack and acts as a barrier in stress conditions such as light, heat and sound. Various microbial EPSs are produced industrially and used in food, petroleum, chemistry and cosmetics for various purposes. They are used in food industry as stabilizer, emulsifier, gelling agent, humectant, thickener etc. Xanthan and gellan are the most extensively used EPSs for these purposes. Other important EPSs such as dextran, curdlan, levan, pullulan and bacterial alginates have also limited applications in the industry. In this review, features and importance of microbial EPSs are discussed.

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Exopolysaccharides produced by the recently described halophilic bacteria Halomonas ventosae and Halomonas anticariensis

Cited by 151 publications

References 46 publications

Genetic and phenotypic analysis of the GacS/GacA system in the moderate halophile Halomonas anticariensis

Genetic and phenotypic analysis of the GacS/GacA system in the moderate halophile Halomonas anticariensis

Halomonas fontilapidosi sp. nov., a moderately halophilic, denitrifying bacterium

Mikrobiyel ekzopolisakkaritler

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