Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties

Bai, Yingying; Luo, Baolong; Zhang, Yan; Li, Xu; Wang, Zhen; Shan, Yuqing; Lü, Min; Tian, Fengwei; Ni, Yuwen

doi:10.1016/j.ijbiomac.2021.06.152

Cited by 16 publications

(11 citation statements)

References 44 publications

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“…Notably, in comparison to previous data on mannose units, the C-2 carbon signal of 79.37 ppm shifted downward, indicating a substitution of residue B at the C-2 position. Based on these analyses, residue B was identified as →2)-α- D -Man p -(1→ ( Wold et al, 2018 ; Bai et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, the spherical structure of EPS-E8 clearly differed from that of previously reported LAB-EPSs. For instance, EPS-C47 has a stiff-like, smooth and flake-like, and layered structure (Ayyash et al, 2020c), EPS-M41 has a compact, stiff, and layer-like structure (Ayyash et al, 2020a), while the surface of EPS-1 obtained from Pediococcus acidilactici MT41-11 is rough, and EPS-1 is attached to irregular shapes of differently sized blocks, and shows flaky appearance (Bai et al, 2021). Topographical AFM images of EPS-E8 are presented in Figures 5C,D.…”

Section: Morphological Characteristicsmentioning

confidence: 99%

“…As biologically synthesized macromolecules, exopolysaccharides (EPSs) are loosely attached to the bacterial cell wall or dispersed into their surroundings during the growth phase of microorganisms ( Bai et al, 2021 ; Buksa et al, 2021 ). Depending on the diversity of monosaccharide subunits and their biosynthesis pathways, EPSs are classified into two types: homopolysaccharides, which contain repeats of one type of monosaccharide, and heteropolysaccharides, which contain two or more monosaccharides.…”

Section: Introductionmentioning

confidence: 99%

“… Ayyash et al (2020a) found that EPS isolated from P. pentosaceus M41 exhibited noteworthy antitumor, antioxidant, antidiabetic, and antibacterial activities. Bai et al (2021) reported that EPS secreted by P. acidilactici MT41-11 could be a potential antioxidant agent, and a prebiotic substance added to food. P. pentosaceus is a Gram-positive, facultatively anaerobic, non-motile, and non-spore-forming species, that is crucial in the fermentation of Chinese cereal vinegar ( Gong et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Exopolysaccharide Produced by Pediococcus pentosaceus E8: Structure, Bio-Activities, and Its Potential Application

Jiang

Gan

et al. 2022

Front. Microbiol.

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The novel exopolysaccharide EPS-E8, secreted by Pediococcus pentosaceus E8, was obtained by anion-exchange and gel filtration chromatography. Structural analyses identified EPS-E8 as a heteropolysaccharide containing mannose, glucose, and galactose. Its major backbone consists of →2)-α-D-Manp-(1→2,6)-α-D-Glcp-(1→6)-α-D-Manp-(1→, and its molecular weight is 5.02 × 104 g/mol. Using atomic force microscopy and scanning electron microscopy, many spherical and irregular reticular-like shapes were observed in the microstructure of EPS-E8. EPS-E8 has outstanding thermal stability (305.7°C). Both the zeta potential absolute value and average particle diameter increased gradually with increasing concentration. Moreover, at a concentration of 10 mg/ml, the antioxidant capacities of, 1-Diphenyl-2-picrylhydrazyl (DPPH), ABTS and hydroxyl radical were 50.62 ± 0.5%, 52.17 ± 1.4%, and 58.91 ± 0.7%, respectively. EPS-E8 possesses excellent emulsifying properties against several food-grade oils, and its activity is retained under various conditions (temperature, pH, and ionic strength). Finally, we found that EPS-E8 as a polysaccharide-based coating could reduce the weight loss and malondialdehyde (MDA) content of strawberry, as well as preserving the vitamin C and soluble solid content during storage at 20°C. Together, the results support the potential application of EPS-E8 as an emulsifier, and a polysaccharide-based coating in fruit preservation.

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Section: Resultsmentioning

confidence: 99%

Section: Morphological Characteristicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exopolysaccharide Produced by Pediococcus pentosaceus E8: Structure, Bio-Activities, and Its Potential Application

Jiang

Gan

et al. 2022

Front. Microbiol.

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“…enterica (55.18%) and Staph. aureus (54.22%) at 2 mg/mL [39]. The EPS obtained from Weissella confusa MD1 showed excellent antibio lm activity against various pathogens like Staph.…”

Section: Discussionmentioning

confidence: 98%

Structural characterization and in vivo anti-inflammatory activity of EPS from Pediococcus pentosaceus 4412 in LPS-induced mice model

Angelin,

Kavitha

2023

Preprint

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Exopolysaccharides are postbiotics secreted by lactic acid bacteria that contribute to bountiful health-promoting properties. In the present investigation, exopolysaccharide (EPS 4412) extracted and purified from Pediococcus pentosaceus 4412 was subjected to structural characterization such as scanning electron microscopy, atomic force microscopy, x-ray diffraction, and thermogravimetric analysis which depicted the EPS 4412 as a porous fibrous microstructure, irregular and smooth surface, semi-crystalline, with thermal stability at 252.86°C. Anti-biofilm activity of EPS 4412 exhibited the maximum adhesion inhibition against Staphylococcus aureus (76.07 ± 2.03%), Bacillus cereus (71.86 ± 0.89%), Salmonella enterica (50.5 ± 0.57%), and Shigella flexneri (38.84 ± 0.38%) at 4mg/mL concentration. At 10 mg/mL concentration, the EPS 4412 displayed moderate DPPH radical scavenging activity of 55.62 ± 0.85% and a reducing power of 1.54 ± 0.03 at OD700 nm. In vivo, the anti-inflammatory activity of EPS 4412 in LPS-induced C57BL/6 mice revealed elevated body weights, thymus, and spleen indices, increased glutathione peroxidase and catalase activities, and reduced malondialdehyde levels. The EPS 4412 had substantially upregulated anti-inflammatory IL-10 and downregulated the pro-inflammatory cytokines, IL-6, and TNF-α to alleviate the inflammatory process. Histopathological examination of the intestine, liver, and spleen tissues disclosed the protective effect of EPS 4412. Thus, EPS from Ped. pentosaceus 4412 has the potential to regulate inflammatory responses, alleviate oxidative stress, and protect organs. EPS 4412 could be employed as a natural food additive and anti-inflammatory drug in the food and pharmaceutical industries.

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Structural Characterization and Functional Studies of Exopolysaccharide by Native Lacticaseibacillus rhamnosus P14 Isolated from the Moroccan Region

Sihame,

Zakaria,

Khalil

et al. 2024

Curr Microbiol

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Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties

Cited by 16 publications

References 44 publications

Exopolysaccharide Produced by Pediococcus pentosaceus E8: Structure, Bio-Activities, and Its Potential Application

Exopolysaccharide Produced by Pediococcus pentosaceus E8: Structure, Bio-Activities, and Its Potential Application

Structural characterization and in vivo anti-inflammatory activity of EPS from Pediococcus pentosaceus 4412 in LPS-induced mice model

Structural Characterization and Functional Studies of Exopolysaccharide by Native Lacticaseibacillus rhamnosus P14 Isolated from the Moroccan Region

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