Exopolysaccharide production by a marine Pseudoalteromonas sp. strain isolated from Madeira Archipelago ocean sediments

Unusual composition of an exopolymer (EP) from an obligate halophilic bacterium Chromohalobacter canadensis 28 has triggered an interest in development of an effective bioreactor process for its production. Its synthesis was investigated in 2‐L bioreactor at agitation speeds at interval 600‐1000 rpm, at a constant air flow rate of 0.5 vvm; aeration rates of 0.5, 1.0, and 1.5 vvm were tested at constant agitation rate of 900 rpm. EP production was affected by both, agitation and aeration. As a result twofold increase of EP yield was observed and additionally increased up to 3.08 mg/mL in a presence of surfactants. For effective scale‐up of bioreactors mass transfer parameters were estimated and lowest values of KLa obtained for the highest productivity fermentation was established. Emulsification activity of EP exceeded that of trade hydrocolloids xanthan, guar gum, and cellulose. A good synergism between EP and commercial cellulose proved its potential exploration as an enhancer of emulsifying properties of trade emulsions. A pronounced lipophilic effect of EP was established toward olive oil and liquid paraffin. Cultivation of human keratinocyte cells (HaCaT) with crude EP and purified γ‐polyglutamic acid (PGA) showed higher viability than control group.

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“…The process with Pseudoalteromonas sp . was shorter (17 h) than that with C. canadensis 28, however, it has performed at 0% NaCl [27].…”

Section: Resultsmentioning

confidence: 99%

High bioreactor production and emulsifying activity of an unusual exopolymer by Chromohalobacter canadensis 28

Radchenkova

Boyadzhieva

Hasköylü

et al. 2020

Engineering in Life Sciences

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“…The presence of Fuc has been shown to be very important for some biological activities [ 49 ]. Although considered as a rare sugar together with Rha [ 49 ], they have both already been described in EPS from Pseudoalteromonas and Polaribacter strains isolated from Antarctic sea water, and sea ice and ocean sediments [ 50 , 51 ] as well as in Vibrio parahaemolyticus EPS [ 52 ] and that of Enterobacter A47 [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Bioprospecting for Exopolysaccharides from Deep-Sea Hydrothermal Vent Bacteria: Relationship between Bacterial Diversity and Chemical Diversity

et al. 2017

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Many bacteria biosynthesize structurally diverse exopolysaccharides (EPS) and excrete them into their surrounding environment. The EPS functional features have found many applications in industries such as cosmetics and pharmaceutics. In particular, some EPS produced by marine bacteria are composed of uronic acids, neutral sugars, and N-acetylhexosamines, and may also bear some functional sulfate groups. This suggests that they can share common structural features with glycosaminoglycans (GAG) like the two EPS (HE800 and GY785) originating from the deep sea. In an attempt to discover new EPS that may be promising candidates as GAG-mimetics, fifty-one marine bacterial strains originating from deep-sea hydrothermal vents were screened. The analysis of the EPS chemical structure in relation to bacterial species showed that Vibrio, Alteromonas, and Pseudoalteromonas strains were the main producers. Moreover, they produced EPS with distinct structural features, which might be useful for targeting marine bacteria that could possibly produce structurally GAG-mimetic EPS.

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“…These organisms may have contributed to the higher bioavailability of the oil components through the production of extracellular polymeric substances (EPS) that have the potential to serve as a biosurfactant ( 16 ), which may, in part, explain the limited differences observed in the chemistry of the O and OD tanks. A previous study demonstrated that some Pseudoalteromonas strains are capable of the very effective production of EPS in as short a period as one day ( 49 ). In a study on bacterial communities during an experimental oil spill in the North Sea by Chronopoulou et al ( 16 ), strains assigned to Pseudoalteromonas constituted the majority of those isolated and were responsible for the utilization of PAHs, and branched and straight-chain alkanes in the early stages of oil contamination.…”

Section: Discussionmentioning

confidence: 99%

Capturing Early Changes in the Marine Bacterial Community as a Result of Crude Oil Pollution in a Mesocosm Experiment

et al. 2017

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The results of marine bacterial community succession from a short-term study of seawater incubations at 4°C to North Sea crude oil are presented herein. Oil was used alone (O) or in combination with a dispersant (OD). Marine bacterial communities resulting from these incubations were characterized by a fingerprinting analysis and pyrosequencing of the 16S rRNA gene with the aim of 1) revealing differences in bacterial communities between the control, O treatment, and OD treatment and 2) identifying the operational taxonomic units (OTUs) of early responders in order to define the bacterial gene markers of oil pollution for in situ monitoring.After an incubation for 1 d, the distribution of the individual ribotypes of bacterial communities in control and oil-treated (O and OD) tanks differed. Differences related to the structures of bacterial communities were observed at later stages of the incubation. Among the early responders identified (Pseudoalteromonas, Sulfitobacter, Vibrio, Pseudomonas, Glaciecola, Neptunomonas, Methylophaga, and Pseudofulvibacter), genera that utilize a disintegrated biomass or hydrocarbons as well as biosurfactant producers were detected. None of these genera included obligate hydrocarbonoclastic bacteria (OHCB). After an incubation for 1 d, the abundances of Glaciecola and Pseudofulvibacter were approximately 30-fold higher in the OD and O tanks than in the control tank. OTUs assigned to the Glaciecola genus were represented more in the OD tank, while those of Pseudofulvibacter were represented more in the O tank. We also found that 2 to 3% of the structural community shift originated from the bacterial community in the oil itself, with Polaribacter being a dominant bacterium.

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Exopolysaccharide production by a marine Pseudoalteromonas sp. strain isolated from Madeira Archipelago ocean sediments

Cited by 42 publications

References 31 publications

High bioreactor production and emulsifying activity of an unusual exopolymer by Chromohalobacter canadensis 28

High bioreactor production and emulsifying activity of an unusual exopolymer by Chromohalobacter canadensis 28

Bioprospecting for Exopolysaccharides from Deep-Sea Hydrothermal Vent Bacteria: Relationship between Bacterial Diversity and Chemical Diversity

Capturing Early Changes in the Marine Bacterial Community as a Result of Crude Oil Pollution in a Mesocosm Experiment

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