Exopolysaccharide Produced by Lactobacillus Plantarum Induces Maturation of Dendritic Cells in BALB/c Mice

Yan-jun, Tang; Dong, Wei; Wan, Keyu; Zhang, Ligang; Li, Chun; Zhang, Lili; Liu, Ning

doi:10.1371/journal.pone.0143743

Cited by 30 publications

(21 citation statements)

References 44 publications

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“…This demonstrates that many LAB strains might have TLR independent health benefits. Moreover, our data suggest that every single tested LAB has a unique PRR signalling profile which might be explained by expression of different ligands on the surface of the bacteria or secretion of PRR-stimulating bactericides or other immune active bacterial products 48 49 50 51 52 .…”

Section: Discussionmentioning

confidence: 88%

Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation

Ren

Zhang

Haan

et al. 2016

Sci Rep

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Although many lactic acid bacteria (LAB) influence the consumer’s immune status it is not completely understood how this is established. Bacteria-host interactions between bacterial cell-wall components and toll-like receptors (TLRs) have been suggested to play an essential role. Here we investigated the interaction between LABs with reported health effects and TLRs. By using cell-lines expressing single or combination of TLRs, we show that LABs can signal via TLR-dependent and independent pathways. The strains only stimulated and did not inhibit TLRs. We found that several strains such as L. plantarum CCFM634, L. plantarum CCFM734, L. fermentum CCFM381, L. acidophilus CCFM137, and S. thermophilus CCFM218 stimulated TLR2/TLR6. TLR2/TLR6 is essential in immune regulatory processes and of interest for prevention of diseases. Specificity of the TLR2/TLR6 stimulation was confirmed with blocking antibodies. Immunomodulatory properties of LABs were also studied by assessing IL-10 and IL-6 secretion patterns in bacteria-stimulated THP1-derived macrophages, which confirmed species and strain specific effects of the LABs. With this study we provide novel insight in LAB specific host-microbe interactions. Our data demonstrates that interactions between pattern recognition receptors such as TLRs is species and strain specific and underpins the importance of selecting specific strains for promoting specific health effects.

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Section: Discussionmentioning

confidence: 88%

Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation

Ren

Zhang

Haan

et al. 2016

Sci Rep

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“…[45][46][47] Mannose has been reported to be a minor component of the EPSs produced by LAB. 44,48,49) In addition, with respect to LAB-derived EPSs having a high mannose content, 46,50,51) the mannose content in the EPS produced by Lb. mucosae DPC 6426, Lb.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand

Panthavee

Noda

Danshiitsoodol

et al. 2017

Biological & Pharmaceutical Bulletin

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In the present study, we have obtained two exopolysaccharide (EPS)-producing thermophilic lactic acid bacteria (LAB) that were isolated from tropical fruits of Thailand. The two strains, designated LY45 and PY45, were identified as Pediococcus pentosaceus and Lactobacillus amylovorus, respectively. Both plantderived LAB strains, which produce neutral EPSs together with the acidic one, can grow vigorously at 45°C and even at 50°C. Hyaluronidase (EC 3.2.1.35), which catalyzes the degradation of hyaluronic acid, activates an inflammatory reaction. Interestingly, EPSs produced by the LY45 and PY45 strains were found to inhibit hyaluronidase activity at the same order of IC 50 values as did sodium cromoglicate and dipotassium glycyrrhizinate, which are well-known as anti-inflammatory agents. The LY45-derived neutral EPS consists of glucose and mannose as monosaccharide components, whereas the acidic one contains mainly mannose, together with glucose and galactose. On the other hand, although Lactobacillus amylovorus PY45 also produces neutral and acidic EPSs, the main monosaccharide in both EPSs is mannose, and glucose is a minor component. Furthermore, the PY45 strain may be probiotically and industrially useful because the microorganism can utilize starch and glycogen as carbon sources. Key words exopolysaccharide; Lactobacillus amylovorus; Pediococcus pentosaceus; thermophilic lactic acid bacteria; anti-inflammatory substanceTraditionally, lactic acid bacteria (LAB) have been useful for producing fermented foods, such as yogurt, kimchi, and cheese. LAB-Gram-positive bacteria-are non-pathogenic and are generally recognized as safe (GRAS) microorganisms; therefore, they have been the focus of attention for their industrial importance. 1)Microorganisms that contribute to human health are called probiotics. This word is internationally defined as "living microorganisms conferring a health benefit on the host when administered in adequate amounts."2) We have isolated many kinds of LAB strains from plant sources such as vegetables, fruits, flowers, and medicinal plants to establish a plantderived LAB library with more than 600 strains. We have demonstrated that some strains stocked in the LAB library are useful for enhancing intestinal immunity, improving liver function, and preventing metabolic syndrome. [3][4][5][6] LAB fermentation often proceeds even at low temperatures, causing the overgrowth of LABs. As the result, the degradation of flavors is proceeded and affected with taste of the fermented foods. Since thermophilic LAB strains hard to ferment at lower temperatures, therefore, if the thermophile ones are successfully isolated, they will contribute to prevent over-fermentation. In the present study, we isolated 28 strains of thermophilic LAB from tropical fruits of Thailand. In the isolates, we found two exopolysaccharide (EPS)-producing LAB strains. The LY45 strain was isolated from lychee, and the PY45 strain was obtained from pineapple. These strains were identified as Pediococcus (P.) pentosaceus and Lactob...

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“…plantarum producing functional EPSs, such as radical scavengers, dendritic cell maturation induction, and anti-bacterial activities. [55][56][57] The investigation of physiological functions of the SN35Nderived EPS, such as anti-allergy, anti-helicobacter pylori, anti-ulcer, and anti-viral activities, is in progress.…”

Section: Eps-biosynthesizing Gene Cluster Detected In the Lb Plantarmentioning

confidence: 99%

Characterization of the SN35N Strain-Specific Exopolysaccharide Encoded in the Whole Circular Genome of a Plant-Derived <i>Lactobacillus plantarum</i>

Noda

Shiraga

Kumagai

et al. 2018

Biological & Pharmaceutical Bulletin

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Lactobacillus plantarum SN35N, which has been previously isolated from pear, secretes exopolysaccharide (EPS). The aim of the present study is to characterize the EPS chemically and to find the EPS-biosynthesizing gene cluster. The present study demonstrates that the strain produces an acidic EPS carrying phosphate residue, which is composed of glucose, galactose, and mannose at a molecular ratio of 15.0 : 5.7 : 1.0. We also show that acidic EPS strongly inhibits the catalytic activity of hyaluronidase (EC 3.2.1.35), promoting an inflammatory reaction. In the present study, we also determined the complete genome sequence of the SN35N strain, demonstrating that the genome is a circular DNA with 3267626 bp, and the number of predicted coding genes is 3146, with a GC content of 44.51%. In addition, the strain harbors four plasmids, designated pSN35N-1, -2, -3, and -4. Although four EPS-biosynthesizing genes, designated lpe1, lpe2, lpe3, and lpe4, are present in the SN35N chromosomal DNA, another EPS gene cluster, lpe5, is located in the pSN35N-3 plasmid, composed of 35425 bp. EPS low-producing mutants, which were obtained by treating SN35N cells with novobiocin, lost the lpe5 gene cluster in the plasmid-curing experiment, suggesting that the gene cluster for the biosynthesis of acidic EPS is present in the plasmid. The present study shows the chemical characterization of the acidic EPS and its inhibitory effect to the hyaluronidase.

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Exopolysaccharide Produced by Lactobacillus Plantarum Induces Maturation of Dendritic Cells in BALB/c Mice

Cited by 30 publications

References 44 publications

Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation

Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation

Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand

Characterization of the SN35N Strain-Specific Exopolysaccharide Encoded in the Whole Circular Genome of a Plant-Derived <i>Lactobacillus plantarum</i>

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