Exopolysaccharide Produced by Lactobacillus paracasei IJH-SONE68 Prevents and Improves the Picryl Chloride-Induced Contact Dermatitis

Noda, Masafumi; Sultana, Nasrin; Hayashi, Ikue; Fukamachi, Mitsuhiro; Sugiyama, Masanori

doi:10.3390/molecules24162970

Cited by 20 publications

(24 citation statements)

References 55 publications

(71 reference statements)

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“…In an in vivo model of AD on BALB/cAJcl mice, the oral administration of an exopolysaccharide (EPS) produced by Lactobacillus paracasei reduced ear swelling, produced a repression of ear interleukin-4 (T helper (Th) 2 cytokine) mRNA and decreased serum immunoglobulin E levels. These results suggest that Lactobacillus paracasei -derived EPS inhibits the catalytic activity of hyaluronidase promoting inflammatory reactions and is useful for improving type I and type IV allergies, including AD [ 69 ].…”

Section: Resultsmentioning

confidence: 99%

Atopic Dermatitis as a Multifactorial Skin Disorder. Can the Analysis of Pathophysiological Targets Represent the Winning Therapeutic Strategy?

et al. 2020

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Atopic dermatitis (AD) is a pathological skin condition with complex aetiological mechanisms that are difficult to fully understand. Scientific evidence suggests that of all the causes, the impairment of the skin barrier and cutaneous dysbiosis together with immunological dysfunction can be considered as the two main factors involved in this pathological skin condition. The loss of the skin barrier function is often linked to dysbiosis and immunological dysfunction, with an imbalance in the ratio between the pathogen Staphylococcus aureus and/or other microorganisms residing in the skin. The bibliographic research was conducted on PubMed, using the following keywords: ‘atopic dermatitis’, ‘bacterial therapy’, ‘drug delivery system’ and ‘alternative therapy’. The main studies concerning microbial therapy, such as the use of bacteria and/or part thereof with microbiota transplantation, and drug delivery systems to recover skin barrier function have been summarized. The studies examined show great potential in the development of effective therapeutic strategies for AD and AD-like symptoms. Despite this promise, however, future investigative efforts should focus both on the replication of some of these studies on a larger scale, with clinical and demographic characteristics that reflect the general AD population, and on the process of standardisation, in order to produce reliable data.

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Section: Resultsmentioning

confidence: 99%

Atopic Dermatitis as a Multifactorial Skin Disorder. Can the Analysis of Pathophysiological Targets Represent the Winning Therapeutic Strategy?

et al. 2020

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“…In fact, one of the EPSs produced by Lb. paracasei IJH-SONE68 has been reported to display prevention effect in picryl chloride-induced contact dermatitis model mice [ 25 ]. Furthermore, a clinical trial in subjects with allergic conditions using the IJH-SONE68 strain is in progress (UMIN Clinical Trials Registry, trial No.…”

Section: Discussionmentioning

confidence: 99%

“…Prior to the molecular mass and constitution sugar analyses, the GI-containing fraction was further purified based on the EPS purification procedure described previously [ 25 ]: the concentrated GI fraction prepared from the culture supernatant of BM53-1 was mixed with the same volume of acetone and incubated overnight at 4 °C. The precipitate was collected and dissolved into a 50 mM Tris-HCl buffer (pH 8.0) and treated with nuclease and proteinase K. The GI collected by ethanol precipitation was dissolved into distilled water and applied to the anion exchange resin (TOYOPEARL DEAE-650M, Tosoh Bioscience, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…We have isolated many kinds of plant-derived LAB strains from fruits, vegetables, flowers, and medicinal plants. It has been reported that some LAB strains stored in the library exhibit preventive and presymptomatic medicinal properties [ 22 , 23 , 24 , 25 , 26 ]. Furthermore, we have found the plant-derived-LAB strains that produce anti-bacterial substances effective against cariogenic S. mutans [ 27 ] and Porphyromonas gingivalis , which causes periodontal inflammatory diseases [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Lactobacillus reuteri BM53-1 Produces a Compound That Inhibits Sticky Glucan Synthesis by Streptococcus mutans

et al. 2021

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Cariogenic bacteria, such as Streptococcus (S.) mutans and S. sobrinus, produce insoluble and sticky glucans as a biofilm material. The present study demonstrates that a lactic acid bacterium (LAB) named BM53-1 produces a substance that inhibits the sticky glucan synthesis. The BM53-1 strain was isolated from a flower of Actinidia polygama and identified as Lactobacillus reuteri. The substance that inhibits sticky glucan synthesis does not exhibit antibacterial activity against S. mutans. The cariogenic S. mutans produces glucans under the control of three glucosyltransferase (GTF) enzymes, named GtfB, GtfC, and GtfD. Although GtfB and GtfC produce insoluble glucans, GtfD forms soluble glucans. Through quantitative reverse-transcriptional (qRT)-PCR analysis, it was revealed that the BM53-1-derived glucan-production inhibitor (GI) enhances the transcriptions of gtfB and gtfC genes 2- to 7-fold at the early stage of cultivation. However, that of gtfD was not enhanced in the presence of the GI, indicating that the glucan stickiness produced by S. mutans was significantly weaker in the presence of the GI. Our result demonstrates that Lb. reuteri BM53-1 is useful to prevent dental caries.

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“…We have already isolated over 1000 LAB strains from many plant sources and stored them in a plant-derived LAB library. We have found that some of the stored LAB strains may be useful for preventive medicine, such as immune modulation, reduction of obesity, and anti-allergy [17][18][19][20]. Interestingly, the yogurt manufactured with Lactobacillus (Lb.)…”

Section: Introductionmentioning

confidence: 99%

Improvement of Alcohol-Poisoning Symptoms in Mice by the Oral Administration of Live Lactobacillus plantarum SN13T Cells

Noda

Maruyama

Danshiitsoodol

et al. 2020

IJMS

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A clinical study carried out previously by our group has demonstrated that yogurt manufactured with a plant-derived lactic acid bacterium, Lactobacillus plantarum SN13T, significantly reduces the γ-glutamyl transpeptidase (γ-GTP) level as a liver-function parameter. In the present study, we show that with the oral administration of live SN13T cells, alcohol-poisoning symptoms in mice are improved, and the condition does not become fatal. However, prior to the simultaneous administration with ethanol, when the cells were heat-killed or sonicated, the improvement was not observed, and almost all of the mice died. In addition, the dysbiosis of the intestinal microbiota observed in the mice administered with ethanol was restored by simultaneous administration with live SN13T cells. Furthermore, by analyzing the metabolites detected in contents from the mouse cecum, it was demonstrated that the increase in nonvolatile putrefactive amines observed in the ethanol-administration group was reduced by simultaneous administration with live SN13T cells. Judging from these results, the lactic acid bacterial cells capable of reaching the living bowels prevent ethanol-induced poisoning and restore the intestinal microbiota.

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Exopolysaccharide Produced by Lactobacillus paracasei IJH-SONE68 Prevents and Improves the Picryl Chloride-Induced Contact Dermatitis

Cited by 20 publications

References 55 publications

Atopic Dermatitis as a Multifactorial Skin Disorder. Can the Analysis of Pathophysiological Targets Represent the Winning Therapeutic Strategy?

Atopic Dermatitis as a Multifactorial Skin Disorder. Can the Analysis of Pathophysiological Targets Represent the Winning Therapeutic Strategy?

Lactobacillus reuteri BM53-1 Produces a Compound That Inhibits Sticky Glucan Synthesis by Streptococcus mutans

Improvement of Alcohol-Poisoning Symptoms in Mice by the Oral Administration of Live Lactobacillus plantarum SN13T Cells

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