Exopolysaccharide produced by Lactiplantibacillus plantarum RO30 isolated from Romi cheese: characterization, antioxidant and burn healing activity

Abstract

Microbial exopolysaccharides (EPSs) extracted from lactic acid bacteria (LAB) are generally recognized as safe. They have earned popularity in recent years because of their exceptional biological features. Therefore, the present study main focus was to study EPS-production from probiotic LAB and to investigate their antioxidant and burn wound healing efficacy. Seventeen LAB were isolated from different food samples. All of them showed EPS-producing abilities ranging from 1.75 ± 0.05 to 4.32 ± 0.12 g/l. RO30 isolate (from Romi cheese) was chosen, due to its ability to produce the highest EPS yield (4.23 ± 0.12 g/l). The 16S rDNA sequencing showed it belonged to the Lactiplantibacillus plantarum group and was further identified as L. plantarum RO30 with accession number OL757866. It displayed well in vitro probiotic properties. REPS was extracted and characterized. The existence of COO−, OH and amide groups corresponding to typical EPSs was confirmed via FTIR. It was constituted of glucuronic acid, mannose, glucose, and arabinose in a molar ratio of 2.2:0.1:0.5:0.1, respectively. The average molecular weight was 4.96 × 104 g/mol. In vitro antioxidant assays showed that the REPS possesses a DPPH radical scavenging ability of 43.60% at 5 mg/ml, reducing power of 1.108 at 10 mg/ml, and iron chelation activity of 72.49% and 89.78% at 5 mg/ml and 10 mg/ml, respectively. The healing efficacy of REPS on burn wound models in albino Wistar rats showed that REPS at 0.5% (w/w) concentration stimulated the process of healing in burn areas. The results suggested that REPS might be useful as a burn wound healing agent.

Functional features of the exopolysaccharide extracts produced by Lactiplantibacillus strains isolated from table olives

“…There is plenty of well-described evidence of the antioxidant activity of EPSs synthesised by Lactobacillus species, such as the EPSs produced by L. helveticus MB2–1, L. acidophilus P185, L. plantarum S123, L. plantarum RO30, L. plantarum MC5, L. casei NA-2, and L. pentosus . 23–29 Data obtained in this work showed the great antioxidant activity of many of the EPS crude extracts obtained from Lactiplantibacillus strains, especially LPG1.…”

Structure and Properties of Exopolysaccharide Produced by Gluconobacter frateurii and Its Potential Applications

“…The scavenging abilities of P-EPS and C-EPS were 80.32 ± 1.67% and 88.12 ± 0.82% when the concentration reached 5.0 mg/mL. The study of Elmansy et al [45] showed that Lac. plantarum RO30 EPS had antioxidant activity when the EPS concentration reached 5 mg/mL; the ABTS radical clearance rate was 49.1% ± 1.32%.…”

Selenium nanoparticles coated bacterial polysaccharide with potent antimicrobial and anti-lung cancer activities

“…The blood hemolytic activity of EPS producers was assessed by inoculating the isolates onto blood agar plates containing 5.0% sheep blood. After 24 h of incubation, the plates were checked for beta, alpha, or gamma hemolysis 24 .…”

“…The potent EPS producer with promising antimicrobial activity were tested for their probiotic tolerance properties against different acid and bile salts according to Nath et al 11 and Yadav et al 24 respectively.…”