Exopolysaccharide from Lactobacillus plantarum LRCC5310 offers protection against rotavirus-induced diarrhea and regulates inflammatory response

Abstract

We aimed to determine the effects of Lactobacillus strains against rotaviral infections. Rotaviruses are the major causative agent of acute gastroenteritis in infants and children worldwide. However, to date, no specific antiviral drugs for the treatment of rotavirus infection have been developed. We identified 263 Lactobacillus strains from 35 samples of the traditional Korean fermented vegetable food kimchi. Among them, Lactobacillus plantarum LRCC5310, more specifically the exopolysaccharides produced by these cells, were shown to have an antiviral effect against human rotavirus Wa strain in vitro. In vivo, the oral administration of exopolysaccharides for 2 d before and 5 d after mouse infection with the murine rotavirus epidemic diarrhea of infant mice strain led to a decrease in the duration of diarrhea and viral shedding and prevented the destruction of enteric epithelium integrity in the infected mice. We demonstrated here that the exopolysaccharides extracted from L. plantarum LRCC5310 can be used for the effective control of rotavirus infection.

The microbiome of the invertebrate model host Galleria mellonella is dominated by Enterococcus

“…Our data presented here show that, independent of the body site sampled, G. mellonella larvae used for microbiological research are dominated by a single Enterococcus taxon, putatively identified as E. gallinarum or E. saccharolyticus based on the combination of ASV analysis and the curated EzBioCloud 16S rRNA reference database [18], although various other taxa (such as Staphylococcus, Pseudomonas and Enterobacter species) were also frequently found. The simplicity of the larval microbiota has a number of implications for their role as model host animals.…”

Exopolysaccharides from probiotic bacteria and their health potential

“…The EPS of probiotic bacteria Lactobacillus, Leuconostoc and Pediococcus are indicated to show stronger antagonistic activity against human adenovirus type 5 [128]. Lactobacillus plantarum LRCC5310 EPS reduced the duration of diarrhea, limited the epithelial lesions, decreased the rotavirus replication in the intestine and shortened the time to recovery of suckling mice [129]. EPS obtained from Lactobacillus plantarum showed inhibitory effect on transmissible gastroenteritis corona virus proliferation in epithelial swine testicle cell line [130].…”

A Surface Protein From Lactobacillus plantarum Increases the Adhesion of Lactobacillus Strains to Human Epithelial Cells

“…Lactobacillus is the most common probiotics which can confer beneficial effects on their hosts when consumed in adequate amounts. These beneficial benefits may include preventing diarrhea ( Staudacher, 2015 ; Sinclair et al, 2016 ; Kim et al, 2018 ), modulating the intestinal community ( Marteau et al, 2002 ; Xie et al, 2016 ), improving lactose tolerance ( Mustapha et al, 1997 ; Staudacher, 2015 ; Oak and Jha, 2018 ), reducing the serum cholesterol level ( Choi et al, 2015 ; Kobyliak et al, 2016 ), suppressing pathogens ( Kim et al, 2014 ; Tuo et al, 2018 ), and boosting the immune system ( Dimitrijevic et al, 2014 ; Kemgang et al, 2014 ). To maximize these functions, Lactobacillus strains must be able to adhere to and colonize the gastro-intestinal tract ( Ljungh and Wadstrom, 2006 ; Tuo et al, 2018 ).…”