Exopolysaccharide fromGanoderma applanatumas a Promising Bioactive Compound with Cytostatic and Antibacterial Properties

Abstract

A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

Laccase isozymes from Ganoderma lucidum MDU-7: Isolation, characterization, catalytic properties and differential role during oxidative stress

“…ROS are generated during normal cellular metabolism or in some stress conditions, commonly responsible for the aging, DNA damage, and protein damage [19][20][21]. Polysaccharides and some other bioactive metabolites with antioxidative properties from G. lucidum, Cerrena unicolor, G. applanatum, Lentinus edodes and Trametes versicolor have been reported [22][23][24], whereas, laccase has been reported with antiproliferative [25,26], prooxidant [23] and HIV reversetranscriptase inhibitor activity [27]. Earlier, laccase gene from G. lucidum has been cloned and studied for its antioxidant properties [28].…”

New alkaline lipase from Rhizomucor variabilis: Biochemical properties and stability in the presence of microbial EPS

“…It is known that both fungal and bacterial strains have an ability to produce exopolysaccharides (EPSs). These biopolymers possess a number of bioactive properties such as anticancer, immunomodulating, cytotoxic, or antibacterial activities . However, to the best of our knowledge, no properties of naturally occurring fungal and bacterial EPSs in the context of lipases activity stabilization are known.…”

Scale‐up and economic analysis of the production of ligninolytic enzymes from a side‐stream of the organosolv process

“…Additionally, in submerged cultures, basidiomycetes can produce exopolysaccharides (EPS) that may have antibacterial properties . For example, Ganoderma applanatum EPS have shown high toxicity on V. fischeri cells . In the present study, the highest concentration of polysaccharides was observed after 3 and 2 days in fermentations of I. lacteus and G. lucidum , respectively (Fig.…”