Exonuclease III-assisted nucleic acid amplification fluorescence immunoassay for the ultrasensitive detection of chloramphenicol in milk

Sang, Panting; Hu, Zhigang; Cheng, Yuliang; Yu, Hang; Yang, Fangwei; Xie, Yunfei; Yao, Weirong; Guo, Yahui; Qian, He

doi:10.1016/j.snb.2021.130564

Cited by 19 publications

(3 citation statements)

References 16 publications

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“…For example, Yang et al developed a nontoxic fluorescent Cs 2 ZnCl 4 perovskite nanocrystal probe for sensitive norfloxacin (NOR) sensing. Moreover, Sang et al constructed a selective fluorescence immunoassay with CdTe QDs based on nucleic acid amplification for the detection of chloramphenicol (CAP) in skimmed milk. Carbon dots (CDs) have attracted increasing attention owing to their excellent properties such as low toxicity, high biocompatibility, outstanding fluorescence stability, and excellent physiochemical characteristics. , In particular, the excellent optical properties and multicolor tunability of CDs can be used in various applications, such as the recognition of various metal ions, bioimaging, and multiple detections.…”

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confidence: 99%

Laser-Printed Paper-Based Microfluidic Chip Based on a Multicolor Fluorescence Carbon Dot Biosensor for Visual Determination of Multiantibiotics in Aquatic Products

et al. 2022

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Excessive use of antibiotics in aquaculture severely endangers human health and ecosystems, which has raised significant concerns in recent years. However, conventional laboratory-based approaches regularly required time or skilled manpower. Herein, we propose a point-of-care-testing (POCT) biosensor detection device for the simultaneous determination of multiantibiotics without complex equipment or professional operators. A laser-printed paper-based microfluidic chip loaded with multicolor fluorescence nanoprobes (mCD-μPAD) was developed to rapidly detect sulfamethazine (SMZ), oxytetracycline (OTC), and chloramphenicol (CAP) on-site. These “fluorescence off” detection probes composed of carbon dots (CDs) conjugated with aptamers (donor) and MoS2 nanosheets (acceptor) (CD-apt-MoS2) were based on Förster resonance energy transfer. Upon the addition of target antibiotics, the significantly recovered fluorescence signal on the μPAD can be sensitively perceived by employing a 3D-printed portable detection box through a smartphone. Under optimal conditions, this μPAD allowed for a rapid response of 15 min toward SMZ, OTC, and CAP with considerable sensitivities of 0.47, 0.48, and 0.34 ng/mL, respectively. In shrimp samples, the recoveries were 95.2–101.2, 96.4–105, and 96.7–106.1% with RSD below 6%. This paper-based sensor opens an avenue for on-site, high-throughput, and rapid detection methods and can be widely used in POCT in food safety.

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confidence: 99%

Laser-Printed Paper-Based Microfluidic Chip Based on a Multicolor Fluorescence Carbon Dot Biosensor for Visual Determination of Multiantibiotics in Aquatic Products

et al. 2022

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“…In comparison to electrochemical immunosensors, optical immunoassays offer significant advantages, such as low sample matrix effect, straight forward operation procedure, high signal-to-noise ratio, and wide applicability for on-site detection of diverse targets. To enhance the sensitivity in determining ultralow-abundance targets, several effective signal amplification strategies have been integrated with optical immunoassays, including enzyme catalysis, DNA-based amplification, and nanotechnology [ 12 , 13 , 14 , 15 , 16 ]. For instance, semiconductor quantum dots (QDs), carbon dots (CDs), and noble metal nanoclusters have been widely employed as novel probes with attractive photoluminescence features, replacing conventional fluorescent dyes [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Overview on the Development of Alkaline-Phosphatase-Linked Optical Immunoassays

Liu,

Chang,

Lou

et al. 2023

Molecules

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The drive to achieve ultrasensitive target detection with exceptional efficiency and accuracy requires the advancement of immunoassays. Optical immunoassays have demonstrated significant potential in clinical diagnosis, food safety, environmental protection, and other fields. Through the innovative and feasible combination of enzyme catalysis and optical immunoassays, notable progress has been made in enhancing analytical performances. Among the kinds of reporter enzymes, alkaline phosphatase (ALP) stands out due to its high catalytic activity, elevated turnover number, and broad substrate specificity, rendering it an excellent candidate for the development of various immunoassays. This review provides a systematic evaluation of the advancements in optical immunoassays by employing ALP as the signal label, encompassing fluorescence, colorimetry, chemiluminescence, and surface-enhanced Raman scattering. Particular emphasis is placed on the fundamental signal amplification strategies employed in ALP-linked immunoassays. Furthermore, this work briefly discusses the proposed solutions and challenges that need to be addressed to further enhance the performances of ALP-linked immunoassays.

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“…However, the classical immunoassays such as enzyme-linked immunosorbent assay (ELISA), is restricted to limited sensitivity and can only realize single-target detection because of the undifferentiated catalytic behavior of horseradish peroxidase (HRP). Due to the development of DNA signal amplification technologies such as PCR, as well as some isothermal amplification techniques, − ultrasensitive and multiplex testing based on fluorescence immunoassays is becoming possible and popular.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Antibiotics, Mycotoxins, and Hormones in Milk by an 8–17 DNAzyme-Based Enzyme-Linked Immunosorbent Assay

Sang

et al. 2022

J. Agric. Food Chem.

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The simultaneous detection of three kinds of small-molecule contaminants (antibiotics, mycotoxins, and hormones) in milk was realized by using an 8–17 DNAzyme-based fluorescent enzyme-linked immunosorbent assay (ELISA), in which 8–17 DNAzyme was utilized as the catalytic enzyme for amplifying the signal. Compared with the conventional ELISA in which horseradish peroxidase is used as the catalyzing factor, this 8–17 DNAzyme-based ELISA could achieve multicolor signal output with lower detection limits. The linearities for chloramphenicol, 17β-estradiol, and aflatoxin M1 were in the range of 0.3 ng/mL–3 μg/mL, 3 ng/mL–3 μg/mL, and 3 pg/mL–3 ng/mL with quantitation limits of 0.3, 3, and 0.003 ng/mL, respectively. This proposed scheme demonstrated that the 8–17 DNAzyme might be an effective substitute for horseradish peroxidase in ELISA for the development of ultrasensitive and multicolor fluorescence immunoassay, which would stimulate the development of ELISA in a new orientation.

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Exonuclease III-assisted nucleic acid amplification fluorescence immunoassay for the ultrasensitive detection of chloramphenicol in milk

Cited by 19 publications

References 16 publications

Laser-Printed Paper-Based Microfluidic Chip Based on a Multicolor Fluorescence Carbon Dot Biosensor for Visual Determination of Multiantibiotics in Aquatic Products

Laser-Printed Paper-Based Microfluidic Chip Based on a Multicolor Fluorescence Carbon Dot Biosensor for Visual Determination of Multiantibiotics in Aquatic Products

Overview on the Development of Alkaline-Phosphatase-Linked Optical Immunoassays

Simultaneous Determination of Antibiotics, Mycotoxins, and Hormones in Milk by an 8–17 DNAzyme-Based Enzyme-Linked Immunosorbent Assay

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