Exonuclease-assisted cascaded recycling amplification for label-free detection ofDNA

Bi, Sai; Li, Li; Cui, Yupeng

doi:10.1039/c1cc16684k

Cited by 73 publications

(42 citation statements)

References 19 publications

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“…[36][37] Due to its unique enzymatic property, Exo III is utilized to manipulate selective enzymatic digestion of probe after hybridization with target DNA to remove the target binding domain and to realize signal readout coupling with target amplification. Schematic illustration of the proposed AgNC-LMB-based method for label-free detection of sequence-specific DNA via Exo IIIaided signal amplification.…”

Section: Working Principle Of the Proposed Methodsmentioning

confidence: 99%

A novel linear molecular beacon based on DNA-scaffolded silver nanocluster for DNA detection via exonuclease III-assisted cyclic amplification

Yin

2015

RSC Adv.

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Developing label-free molecular beacon (MB)-based methods for DNA detection has been of great significance in bioanalysis because of their simplicity, low cost, and specificity. In this work, we have developed a novel DNA-scaffolded silver nanocluster linear molecular beacon (AgNC-LMB)-based strategy for sequence-specific DNA detection via exonuclease III (Exo III)-assisted signal amplification. The proposed method involves two processes: target-mediated digestion by Exo III and the synthesis of AgNC-LMB as a switch for signal output. Upon hybridization of the rationally designed probe with target, Exo III removes nucleotides from 3' terminus of the probe. The resultant fragment acts as a favorable template to form highly fluorescent DNA/AgNCs. The resulting fluorescence enhancement of the AgNCs provides a quantitative readout proportional to the target concentration in the range of 5 to 300 nM with an LOD of ~ 3.2 nM. This method is simple, cost-effective, highly selective, and free of modification or separation.

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Section: Working Principle Of the Proposed Methodsmentioning

confidence: 99%

A novel linear molecular beacon based on DNA-scaffolded silver nanocluster for DNA detection via exonuclease III-assisted cyclic amplification

Yin

2015

RSC Adv.

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“…Sun et al [118] reported the method for adenosine determina tion based on HCR and EASA. In some cases, the same technique was applied several times to amplify various intermediate compounds [119].…”

Section: Amplification Techniques That Provide Increase In the Analytmentioning

confidence: 99%

Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis

Bodulev

Sakharov

2020

Biochemistry Moscow

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“…6a-c). Besides ExoIII, 117 other DNA-cleaving enzymes (such as FokI restriction endonuclease 118 and Nt.BstNBI nicking enzyme 119 ) have also been utilized for amplified bioanalysis. Besides, the release of PPIX and reset of the system can be logically controlled by AND-NOT and OR-YES logic circuits, respectively.…”

Section: Split G4 Strategymentioning

confidence: 99%

Versatile G-quadruplex-mediated strategies in label-free biosensors and logic systems

Ren

Wang

2015

Analyst

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G-quadruplex (G4), as one of the significant functional nucleic acids (FNAs), has attracted researchers' wide attention, and in particular has been employed for the construction of label-free molecular sensors and logic systems based on the peroxidase-like activity of the G4-hemin complex and G4-enhanced luminescence of G4-binding organic dyes. Its cation-dependent conformation and stability provide opportunities for the recognition of metal ion inputs and application of a split G4 strategy. Moreover, coupling the G4 sequence with other FNAs, e.g. metal ion-dependent DNAzymes and aptamers, has prominently broadened the range of possible targets from metal ions and DNA to diverse proteins and cells. Although there are limitations, such as a low ability of anti-interference and multiplex analysis, the excellent advantages (e.g. simplicity and low cost) endow the G4-mediated strategy with tremendous potential to be further exploited for practical bioanalysis and complicated DNA computing.

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Exonuclease-assisted cascaded recycling amplification for label-free detection ofDNA

Cited by 73 publications

References 19 publications

A novel linear molecular beacon based on DNA-scaffolded silver nanocluster for DNA detection via exonuclease III-assisted cyclic amplification

A novel linear molecular beacon based on DNA-scaffolded silver nanocluster for DNA detection via exonuclease III-assisted cyclic amplification

Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis

Versatile G-quadruplex-mediated strategies in label-free biosensors and logic systems

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