Exogenous spermidine modulates glycosaminoglycan accumulation and epithelial differentiation in chick embryonic skin

Stabellini, G.; Pellati, Agnese; Tosi, Lara; Caruso, Angelo; Carinci, Paolo

doi:10.1002/(sici)1097-010x(19980815)281:6<594::aid-jez7>3.0.co;2-i

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Epithelial-mesenchymal interactions are required for the development of many organs, including those of the gastrointestinal, tegumental, renal and respiratory systems (Peters et al, 1992;Stabellini et al, 1998;Wallner et al, 1998;Hogan, 1999;Quin et al, 1999). They are mediated by soluble and /or membrane-bound mesenchymal factors, which act as signalling molecules (Ohmichi et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, a crucial point to be clarified in embryonic lung development regards the effects of different growth factors on extracellular matrix (ECM) modifications during bronchial branching. We have recently shown that exogenous SPD modulates the accumulation of sulphated GAG in chick embryo skin, leading to an increase of feather formation (Stabellini et al, 1998). The organotypic culture system offers a suitable model to study the interactions of growth factors, including PA, and ECM, and hence the resulting effects on morphogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Epithelial-mesenchymal interactions and lung branching morphogenesis. Role of polyamines and transforming growth factor ß1

Stabellini

Locci²,

Calvitti³

et al. 2009

Eur J Histochem

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Lung branching morphogenesis is a result of epithelial-mesenchymal interactions, which are in turn dependent on extracellular matrix composition and cytokine regulation. Polyamines have recently been demonstrated as able to modify chick embryo skin differentiation. In this work we have examined the effects of putrescine and spermidine during chick embryo lung morphogenesis in organotypic cultures by morphological, histochemical and biochemical examination. To verify the role of polyamines, we used specific inhibitors, such as bis-cyclohexylammonium sulphate and alfa-difluoromethylornithine, and transforming growth factor ß 1 , an ornithine decarboxylase and polyamine stimulator. Our data show that lung morphogenesis is significantly altered following the induced mesenchymal glycosaminoglycan changes. The increase of mesenchymal glycosaminoglycans is correlated with a stimulation of lung development in the presence of polyamines, and with its inhibition when transforming growth factor ß 1 is added to the culture medium. The morphometric data show a uniform 151 increase of both the mesenchyme and epithelial branching with spermidine and putrescine stimulus, whereas the mesenchymal substance alone is significantly increased in apical-median lung sections with transforming growth factor ß 1 and transforming growth factor ß 1 + spermidine lung cultures. Transforming growth factor ß 1 and transforming growth factor ß 1 + spermidine confirm the blocking of epithelial branching formations and fibroblast activation, and show that polyamines are unable to prevent the blocking of epithelial cells due to the inhibitory effect of transforming growth factor ß 1 .

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Epithelial-mesenchymal interactions and lung branching morphogenesis. Role of polyamines and transforming growth factor ß1

Stabellini

Locci²,

Calvitti³

et al. 2009

Eur J Histochem

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“…The regulation of morphogenesis in various organs such as lung, kidney, skin and limbs (Becchetti et al, 1988;Davies et al, 1995, Kulyk andKosher, 1987;Stabellini et al, 1998) is the result of complex processes in which the regulation of the extracellular matrix (ECM) plays a key role. The control of ECM composition has therefore to be considered as a main aspect for both cell strategy and the influence of environmental factors on cellular behavior.…”

Section: Introductionmentioning

confidence: 99%

Glycosidases during chick embryo lung development and their colocalization with proteoglycans and growth factors

Stabellini

Calvitti²,

Baroni³

et al. 2009

Eur J Histochem

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During development, the epithelial component of the lung goes through a complex orderly process of branching, following strict patterns of space and time. Proteoglycans, glycosaminoglycans and growth factors are fundamental components of the extracellular matrix and perform a key role in differentiative processes. The embryonic chick lung shows a specific glycosaminoglycan composition at different levels of branching and at different embryonic stages. Proteoglycan and glycosaminoglycan accumulation is the result of secretion, absorption and degradation processes. In this pathway, enzymes, such as glycosidases, growth factors and cytokines are involved. We examined the behaviour of glycosidases, such as ß-hexosaminidases (ß-N-acetyl-D-glucosaminidase, ß- N-acetyl-D-galactosaminidase), ß-glucuronidase and ß-galactosidase, during the development of the lung bud. Our data show that the activity of the enzymes is closely linked to the processes of epithelial proliferation, bronchial tubule lengthening and infiltration of the surrounding mesenchyme. The glycosaminoglycans colocalize with transforming growth factor ß2 and inter- leukin-1 in the basement membrane and in the mesenchymal areas where the epithelium grows, and are complementary to the presence of the glycosidases. In conclusion, the activity of these glycosidases is spatially and temporally programmed and favors the release of the factors and the events which they influence

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Chick embryo back skin organ and fibroblast cultures. Extracellular matrix changes induced by dialysate fluid and uraemic toxins in relation to proliferation and differentiation processes

et al. 2003

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Exogenous spermidine modulates glycosaminoglycan accumulation and epithelial differentiation in chick embryonic skin

Cited by 3 publications

References 19 publications

Epithelial-mesenchymal interactions and lung branching morphogenesis. Role of polyamines and transforming growth factor ß1

Epithelial-mesenchymal interactions and lung branching morphogenesis. Role of polyamines and transforming growth factor ß1

Glycosidases during chick embryo lung development and their colocalization with proteoglycans and growth factors

Chick embryo back skin organ and fibroblast cultures. Extracellular matrix changes induced by dialysate fluid and uraemic toxins in relation to proliferation and differentiation processes

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