Exogenous phenylalanine application promotes chilling tolerance in tomato fruits during cold storage by ensuring supply of NADPH for activation of ROS scavenging systems

Aghdam, Morteza Soleimani; Moradi, Mohsen; Razavi, Farhang; Rabiei, Vali

doi:10.1016/j.scienta.2018.11.074

Cited by 53 publications

(42 citation statements)

References 38 publications

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“…These were due to the capacity of phenylalanine to increase the activity of antioxidant enzymes and the accumulation of ROS scavenging agents, such as ascorbic acid (AA), phenols, flavonoids and proline. Our results are similar to those of phenylalanine-treated tomatoes, which showed an increase in the membrane integrity and resistance of tissues to chilling injury during storage ( 10 ). Regarding our results, MDA concentrations were lower in phenylalanine-treated fruits, and therefore they better maintained the integrity of the membrane.…”

Section: Resultssupporting

confidence: 88%

“…In this pathway, phenylalanine ammonia lyase (PAL) is the first enzyme that catalyzes the conversion of phenylalanine to ﬂavonoids, phenolics and anthocyanins. Various biotic and abiotic stresses may stimulate PAL activity, which results in the accumulation of bioactive compounds ( 10 ). Recently, the application of phenylalanine solution as preharvest and postharvest fruit treatments has been considered for enhancing the nutritional quality of horticultural crops.…”

Section: Introductionmentioning

confidence: 99%

“…Aghdam et al . ( 10 ) reported that phenylalanine application significantly decreased chilling injury, membrane lipid peroxidation and ROS accumulation in tomato fruits during cold storage. Moreover, in fruits treated with 0.5 mM phenylalanine, the contents of phenols and flavonoids and the PAL activity were higher than in those treated with other treatments, including control fruits.…”

Section: Introductionmentioning

confidence: 99%

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Phenylalanine Alleviates Postharvest Chilling Injury of Plum Fruit by Modulating Antioxidant System and Enhancing the Accumulation of Phenolic Compound

Sogvar

Rabiei

Razavi

et al. 2020

Food Technol. Biotechnol. (Online)

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Research background. Low temperature storage causes chilling injury (CI) in plum (Prunus domestica L.) fruits. Consequently, any treatments with beneficial effects on these symptoms would achieve attention. For this purpose, phenylalanine treatments were applied on ‘Stanley’ plum fruits. The main purpose of the present study was to investigate the influence of the exogenous application of phenylalanine on fruit quality, chilling tolerance, and antioxidant capacity of ‘Stanley’ plums during cold storage. Experimental approach. Phenylalanine at different concentrations were applied on ‘Stanley’ plums. Following phenylalanine application, plums were cold stored. Chilling injury, antioxidant capacity, electrolyte leakage, malondialdehyde, proline, and internal contents of anthocyanin, flavonoids, phenols, ascorbic acid, and some antioxidant enzymes were assessed. Results and conclusions. Phenylalanine treatment signiﬁcantly alleviated chilling injury in plum fruits by enhancing antioxidant capacity and increasing the activity of phenylalanine ammonia lyase enzyme (PAL). Phenylalanine-treated fruits had higher levels of ascorbic acid, anthocyanin, flavonoids, and phenols, as well as a higher total antioxidant activity, than the control fruits during low temperature storage. Phenylalanine at 7.5 mM was the most effective treatment in enhancing the activity of PAL and the accumulation of phenolic compounds and in reducing the severity of chilling injury. Treatments delayed mass loss and maintained fruit firmness. In addition, the application of 7.5 mM phenylalanine improved the activities of antioxidant enzymes (superoxide dismutase, catalase, and ascorbate peroxidase), decreased the accumulation of hydrogen peroxide, and increased the endogenous content of proline. Moreover, phenylalanine maintained membrane integrity, manifested by a reduced electrolyte leakage and malondialdehyde accumulation. Novelty and scientific contribution. In the current study, chilling injury had a positive correlation with the activities of PAL and antioxidant enzymes. However, negative correlations were observed between chilling injury and ascorbic acid content and antioxidant capacity. Considering the results, phenylalanine treatment could be spotted as an encouraging approach to alleviate the severity of chilling injury and thus preserve nutritional quality of plums during low temperature storage.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenylalanine Alleviates Postharvest Chilling Injury of Plum Fruit by Modulating Antioxidant System and Enhancing the Accumulation of Phenolic Compound

Sogvar

Rabiei

Razavi

et al. 2020

Food Technol. Biotechnol. (Online)

Self Cite

View full text Add to dashboard Cite

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“…It has been reported that low temperatures induce cell membrane damage (Aghdam et al, 2019), and MDA is widely adopted as an indicator of oxidative stress and membrane integrity in plants when they respond to stressors (Sato et al, 2011). In our study, under a low temperature, compared with both IN and NN groups, the AN group clearly produced less MDA in both their shoot and root parts (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

Effect of Rhizobium Symbiosis on Low-Temperature Tolerance and Antioxidant Response in Alfalfa (Medicago sativa L.)

Liu

Geng²,

Sha

et al. 2019

Front. Plant Sci.

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Low temperature-induced stress is a major environmental factor limiting the growth and development of plants. Alfalfa ( Medicago sativa L.) is a legume well known for its tolerance of extreme environments. In this study, we sought to experimentally investigate the role of rhizobium symbiosis in alfalfa’s performance under a low-temperature stress condition. To do this, alfalfa “Ladak + ” plants carrying active nodules (AN), inactive nodules (IN), or no nodules (NN) were exposed to an imposed low temperature stress and their survivorship calculated. The antioxidant defense responses, the accumulation of osmotic regulation substances, the cell membrane damage, and the expression of low temperature stress-related genes were determined in both the roots and the shoots of alfalfa plants. We found that more plants with AN survived than those with IN or NN under the same low temperature-stress condition. Greater activity of oxidation protective enzymes was observed in the AN and IN groups, conferring higher tolerance to low temperature in these plants. In addition, rhizobia nodulation also enhanced alfalfa’s ability to tolerate low temperature by altering the expression of regulatory and metabolism-associated genes, which resulted in the accumulation of soluble proteins and sugars in the nodulated plants. Taken together, the findings of this study indicate that rhizobium inoculation offers a practical way to promote the persistence and growth potential of alfalfa “Ladak + ” in cold areas.

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“…An increase in the abundance of the aromatic amino acids phenylalanine and tyrosine, which are the precursors of the shikimate-phenylpropanoid pathway, was also induced by CA. It has been reported that low temperature can increase the accumulation of phenylalanine 34 , and exogenous application of phenylalanine can promote chilling tolerance in tomato by activating ROS-scavenging systems 35 . Our results showed an increased content of tyrosine, which may also act as an important component in the phenylpropanoid pathway for secondary metabolism.…”

Section: Discussionmentioning

confidence: 99%

Combined transcriptomic and metabolomic analyses uncover rearranged gene expression and metabolite metabolism in tobacco during cold acclimation

Chen

Wang

et al. 2020

Sci Rep

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cold temperatures often severely restrict the growth, distribution and productivity of plants. the freezing tolerance of plants from temperate climates can be improved by undergoing periods of cold acclimation (cA). tobacco is an important economic plant and is sensitive to cold stress. However, the dynamic changes and regulatory mechanisms of gene expression and metabolic processes during CA remain largely unknown. In this study, we performed RNA sequencing and metabolomic profiling analyses to identify the genes and metabolites specifically expressed during CA. Our transcriptomic data revealed 6905 differentially expressed genes (DEGs) during CA. Functional annotation and enrichment analyses revealed that the DEGs were involved mainly in signal transduction, carbohydrate metabolism and phenylpropanoid biosynthesis. Moreover, a total of 35 significantly changed metabolites were identified during CA via an LC-MS platform. Many protective metabolites, such as amino acids, carbohydrates, tricarboxylic acid (TCA) cycle intermediates and phenylpropanoid-related substances, were identified during CA. The gene-metabolite network extensively outlined the biological processes associated with the utilization of sugars, activation of amino acid metabolism, tcA cycle and phenylpropanoid biosynthesis in tobacco under cA. the results of our present study provide a comprehensive view of signal transduction and regulation, gene expression and dynamic changes in metabolites during cA. Land plants living in restricted spaces usually encounter environmental stresses that affect their growth and productivity. Among these stressful conditions, cold is one of the major constraining factors that limits the geographical distribution and agricultural productivity of crops 1. Tobacco (Nicotiana tabacum L.), a Solanaceae species, originates from tropical and subtropical America but is now commercially cultivated worldwide due to its high economic value. In addition, it is commonly used as an excellent model plant species for studies on physiological traits and molecular functions. However, tobacco is sensitive to cold stress 2. Cold acclimation (CA) is recognized as a typical process by which many temperate plants develop chilling and freezing tolerance 3. Hence, exploring the molecular mechanism of CA in tobacco is of great value for breeding cold-tolerant varieties for agricultural production. Numerous studies have shown that CA is closely associated with complex changes in the regulation of gene expression, accumulation of protective metabolites and maintenance of metabolic homeostasis 4-6. Many efforts in CA studies of various plant species have suggested that cold induces extensive recombination of gene transcription 7-9. At present, the product of the best-known cold-responsive (COR) gene involved in the CA signalling pathway is the ICE1-CBF-COR module. In this model, Arabidopsis plants exposed to cold stress induce the expression of C-repeat (CRT) binding factors/dehydration-responsive element binding factors (CBFs/DREBs), which ca...

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Exogenous phenylalanine application promotes chilling tolerance in tomato fruits during cold storage by ensuring supply of NADPH for activation of ROS scavenging systems

Cited by 53 publications

References 38 publications

Phenylalanine Alleviates Postharvest Chilling Injury of Plum Fruit by Modulating Antioxidant System and Enhancing the Accumulation of Phenolic Compound

Phenylalanine Alleviates Postharvest Chilling Injury of Plum Fruit by Modulating Antioxidant System and Enhancing the Accumulation of Phenolic Compound

Effect of Rhizobium Symbiosis on Low-Temperature Tolerance and Antioxidant Response in Alfalfa (Medicago sativa L.)

Combined transcriptomic and metabolomic analyses uncover rearranged gene expression and metabolite metabolism in tobacco during cold acclimation

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