Exogenous nitric oxide limits salt-induced oxidative damage in maize by altering superoxide dismutase activity

Klein, Ashwil; Husselmann, Lizex; Keyster, Marshall; Ludidi, Ndiko

doi:10.1016/j.sajb.2017.12.010

Cited by 47 publications

(13 citation statements)

References 35 publications

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“…NO has been reported to induce antioxidant enzyme activity under salt stress [39,63]. In present work, we found that salt stress, ALA and SNP all induced increases of antioxidant enzyme activity in strawberry, but eliminated by Na 2 WO 4 or cPTIO (Fig.…”

Section: Discussionsupporting

confidence: 60%

5-Aminolevulinic Acid-induced Salt Tolerance in Strawberry: Possible Role of Nitric Oxide on Interception of Salty Ions in Roots

He¹,

An²,

Yang³

et al. 2020

Preprint

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Background: 5-Aminolevunic acid (ALA), as a natural non-protein amino acid and the first essential precursor of tetrapyrrole biosynthesis in all living bodies, has been suggested to improve salt tolerance of plants. In the previous work, we reported that ALA induces H2O2 accumulation in roots of strawberry, which is involved in up-regulating Na+ transporter gene expressions to intercept Na+ in roots with less upward transport. However, the signal route is not clear.Results: In this study, we propose that nitric oxide (NO) is involved in ALA signaling cascade. Therefore, we applied sodium nitrosylpentacy (SNP, NO donor), Na2WO4 (NO biosynthetic inhibitor), and 2, 4-carboxyphenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, NO scavenger) to the culture solution when strawberry (Fragaria × ananassa Duch. cv. ‘Benihoppe’) was stressed by 100 mmol L-1 NaCl with or without exogenous ALA. The results reveal that salinity greatly impaired plant growth while 10 mg L-1 ALA or 10 µM SNP ameliorated the inhibition. When 5 µM Na2WO4 or cPTIO was co-treated, the ALA-improved salt tolerance was almost completely eliminated. This suggests that ALA-improved salt tolerance is dependent on NO presence. We found that salinity caused NO, H2O2, Na+ and Cl- increases in the whole plants, while ALA induced additional increases in roots but significant depressions in leaves. These tissue-specific responses to ALA are important for plant salt tolerance. Conclusion: We propose that the regulation of ALA in roots is critical, which is mediated through NO and then H2O2 signal to up-express genes related with Na+ and Cl- transport, selectively retaining Na+ and Cl- in roots with less upward transport. The hypothesis can reasonably explain how ALA-treated plants cope with toxic ions under salinity.

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Section: Discussionsupporting

confidence: 60%

5-Aminolevulinic Acid-induced Salt Tolerance in Strawberry: Possible Role of Nitric Oxide on Interception of Salty Ions in Roots

He¹,

An²,

Yang³

et al. 2020

Preprint

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“…Pretreatment of wheat seedling with glucose activates NR to produce NO under aluminum stress (Sun et al, 2018). It has been reported that endogenous NO production could influence the salinity tolerance of several plants, mostly via stimulation of antioxidant defense mechanisms (Khator et al, 2000;Ali et al, 2017;Gadelha et al, 2017;Klein et al, 2018) and suppression in uptake of toxic ions (Fatma et al, 2016;Gadelha et al, 2017). The UA-mediated endogenous NO production may be partly explained the alleviation of salt stress in rice cultivars in the present study.…”

Section: Potential Mechanism Of Ua-mediated Salt Tolerancementioning

confidence: 49%

Ursolic Acid Limits Salt-Induced Oxidative Damage by Interfering With Nitric Oxide Production and Oxidative Defense Machinery in Rice

Long

Shou²,

Wang

et al. 2020

Front. Plant Sci.

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Crops frequently encounter abiotic stresses, and salinity is a prime factor that suppresses plant growth and crop productivity, globally. Ursolic acid (UA) is a potential signaling molecule that alters physiology and biochemical processes and activates the defense mechanism in numerous animal models; however, effects of UA in plants under stress conditions and the underlying mechanism of stress alleviation have not been explored yet. This study examined the effects of foliar application of UA (100 µM) to mitigate salt stress in three rice cultivars (HZ, 712, and HAY). A pot experiment was conducted in a climate-controlled greenhouse with different salt stress treatments. The results indicated that exposure to NaCl-induced salinity reduces growth of rice cultivars by damaging chlorophyll pigment and chloroplast, particularly at a higher stress level. Application of UA alleviated adverse effects of salinity by suppressing oxidative stress (H 2 O 2 , O 2−) and stimulating activities of enzymatic and non-enzymatic antioxidants (APX, CAT, POD, GR, GSH, AsA, proline, glycinebutane), as well as protecting cell membrane integrity (MDA, LOX, EL). Furthermore, UA application brought about a significant increase in the concentration of leaf nitric oxide (NO) by modulating the expression of NR and NOS enzymes. It seems that UA application also influenced Na + efflux and maintained a lower cytosolic Na + /K + ratio via concomitant upregulation of OsSOS1 and OsHKT1;5 in rice cultivars. The results of pharmacological tests have shown that supply of the NO scavenger (PTI) completely reversed the UA-induced salt tolerance in rice cultivars by quenching endogenous NO and triggering oxidative stress, Na + uptake, and lipid peroxidation. The PTI application with UA and sodium nitroprusside (SNP) also caused growth retardation and a significant increase in Na + uptake and oxidative stress in rice cultivars. This suggests that UA promoted salt tolerance of rice cultivars by triggering NO production and limiting toxic ion and reactive oxygen species (ROS) accumulation. These results revealed that both UA and NO are together required to develop a salt tolerance response in rice.

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“…Following prolonged exposure time, the effect of K. candel leaf extracts on SOD in P. globosa gradually increased, indicating that the degree of lipid peroxidation of algal cells was continuously enhanced [ 51 ]. This resulted in excessive accumulation of active oxygen in P. globosa and consequently increased oxidative stress [ 52 ]. Consequently, SOD activity in P. globosa increased in order to reduce the damage caused by active oxygen.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Inhibitory Effects of Mangrove Leaves and Analysis of Their Active Components on Phaeocystis globosa during Different Stages of Leaf Age

Zhao

Han

Sun

et al. 2018

IJERPH

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The presence of harmful algal blooms (HABs) can cause significant problems to the quality of the water, the marine ecosystems, and the human health, and economy worldwide. Biological remediation can inhibit harmful algal growth efficiently in an environmental-friendly manner. Therefore, the research conducted on biological remediation with regard to the inhibition of HABs is becoming a major focus in marine ecology. To date, no study has been reported with regard to the red tides occurring in mangrove wetlands. Therefore, the present study used two mangrove species, namely Bruguiera gymnorrhiza and Kandelia candel and one harmful algae species Phaeocystis globosa as experimental organisms. The present study determined the inhibitory effects and algae physiology of specific aqueous extracts from mangrove leaves on the viability of harmful algae, and analyzed the main chemical composition of the aqueous extracts by ultra-performance liquid chromatography coupled to high resolution mass spectrometry (UPLC-QTOF-MS). The results indicated that the aqueous extracts from different leaf ages of B. gymnorrhiza and K. candel leaves exhibited apparent inhibitory effects on the growth of P. globosa. The inhibitory effects of B. gymnorrhiza and K. candel leaves aqueous extracts on the growth of P. globosa were in the following order: senescent > mature > young leaves. The levels of the parameters superoxide dismutase (SOD) activity, glutathione (GSH), and malondialdehyde (MDA)content in P. globosa following treatment with B. gymnorrhiza and K. candel leaves aqueous extracts were increased as follows: senescent > mature > young leaves. Simultaneously, the intensity of the ion peaks of the specific secondary metabolites assigned 4 (No.: 4 Rt: 2.83 min), 7 (No.: 7 Rt: 3.14 min), 8 (No.: 8 Rt: 3.24 min), 9 (No.: 9 Rt: 3.82min) and 10 (No.: 10 Rt: 4.10 min) were increased. These metabolites were found in the aqueous extracts from B. gymnorrhiza leaves. The intensities of the ion peaks of the secondary metabolites 7, 8 in the aqueous extracts from the K. candel leaves were also increased. The majority of the substances that inhibited the algae found in the mangrove plants were secondary metabolites. Therefore, we considered that the norsesquiterpenes compounds 4, 8, 9, and 10 and a phenolic glycoside compound 7 were the active constituents in the aqueous extracts of the mangrove leaves responsible for the inhibition of algae growth. This evidence provided theoretical guidance for the development of biological methods to control red tides and for the further use of substances with antiproliferative activity against algae.

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Exogenous nitric oxide limits salt-induced oxidative damage in maize by altering superoxide dismutase activity

Cited by 47 publications

References 35 publications

5-Aminolevulinic Acid-induced Salt Tolerance in Strawberry: Possible Role of Nitric Oxide on Interception of Salty Ions in Roots

5-Aminolevulinic Acid-induced Salt Tolerance in Strawberry: Possible Role of Nitric Oxide on Interception of Salty Ions in Roots

Ursolic Acid Limits Salt-Induced Oxidative Damage by Interfering With Nitric Oxide Production and Oxidative Defense Machinery in Rice

Investigation of the Inhibitory Effects of Mangrove Leaves and Analysis of Their Active Components on Phaeocystis globosa during Different Stages of Leaf Age

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